Streptozotosin ve Nikotinamid/Streptozosin Kullanılarak Sıçanlarda Tip 1 ve Tip 2 Diyabet Geliştirilmesi

Yıl 2017, Sayı: 1, 9 - 17, 01.01.2017

Tugba Gulsun Selma Sahin

Öz

Diyabet dünya çapında hızla büyüyen bir hastalıktır. Diyabet için geliştirilen deneysel hayvan modelleri hastalığın karmaşık yapısını anlamak için faydalıdır. Bu çalışmanın amacı, sırasıyla streptozotosin ve nikotinamid / streptozotosin kullanarak sıçanlarda tip 1 ve tip 2 diyabet geliştirmektir. Bu çalışmanın sonuçları streptozotosin kullanılarak tip 1 diyabet, ve nikotinamid / streptozotosin kullanarak tip 2 diyabet geliştirildiğini göstermiştir. Tip 1 ve tip 2 diyabet geliştirmek için kullanılan bu yöntemler diyabet ile ilgili çalışmalar da kolaylıkla kullanılabilir.

Anahtar Kelimeler

Tip 1 diyabet, Tip 2 diyabet, streptozotosin, nikotinamid, açlık kan şekeri

Development of Type 1 and Type 2 Diabetes in Rats Using Streptozotocin and Nicotinamide/Streptozotocin

Öz

Diabetes mellitus is a rapidly growing disease worldwide. Experimental animal models for diabetes mellitus are beneficial for understanding the complex nature of the disease. The purpose of this study was to develop type 1 and type 2 diabetes mellitus in rats using streptozotocin and nicotinamide/streptozotocin, respectively. Results of this study showed that type 1 diabetes was developed using streptozotocin, and type 2 diabetes using nicotinamide/ streptozotocin. These methods used for the development of type 1 and type 2 diabetes can be easily used in the diabetes- related studies.

Anahtar Kelimeler

Type 1 diabetes, Type 2 diabetes, streptozotocin, nicotinamide, fasting blood glucose

Kaynakça

• Wild S, Roglic G, Green A, Sicree R, King H: Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004, 27(5) 1047-1053.
• Satman I, Yilmaz T, Sengül A, Salman S, Salman F, Uygur S, Bastar I, Tütüncü Y, Sargin M, Dinççag N, Karsidag K, Kalaça S, Ozcan C, King H: Population-based study of diabetes and risk characteristics in Turkey: results of the turkish diabetes epidemiology study (TURDEP). Diabetes Care 2002, 25(9): 1551-1556.
• IDF Diabetes Atlas Seventh.; 2015 June 10. Available from: http://www.idf.org/diabetesatlas/ update-2014.
• Donovan D: Principles of Diabetes Mellitus. Kluwer Academic Publishers; Boston, Dordrecht: London, 2002.
• Mealey BL, Ocampo, GL: Diabetes mellitus and periodontal disease. Periodontology 2000 2007, 44: 127-153.
• Bastaki W, Mothaffer F, Varro J, Al-Ghanim M, Malak L, Ayyash E, Asfar S: Primary hepatic carcinoid tumor. Medical Principles and Practice 2005, 14(4): 288-291.
• Sacks DB, Arnold M, Bakris GL, Bruns DE, Horvath AR, Kirkman MS, Lernmark A, Metzger BE, Nathan DM: Guidelines and recommendations for laboratory analysis in the diagnosis and management of diabetes mellitus. Clinical Chemistry 2011, 57(6): e1-e47.
• American Diabetes Association: Diagnosis and classification of diabetes mellitus. Diabetes Care 2004, 27 (1): S5-S10.
• Krook A: A balancing act of optimising insulin dose and insulin sensitivity in type 1 diabetes. Journal of Endocrinology 2011, 211(1): 1-2.
• Barag SH: Insulin therapy for management of type 2 diabetes mellitus: strategies for initiation and long- term patient adherence. The Journal of the American Osteopathic Association 2011, 111(5): S13-19.
• Karakurt F: Gestasyonel diabetes mellitus tanı ve tedavisi. Yeni Tıp Dergisi 2009, 26 (3): 134-138.
• Buchanan TA, Xiang AH: Gestational diabetes mellitus. The Journal of Clinical Investigation 2005, 115(3): 485-491.
• American Diabetes Association: Diagnosis and classification of diabetes mellitus. Diabetes Care 2011, 34(1): S62-S69.
• Silva FR, Szpoganicz B, Pizzolatti MG, Willrich MA, de Sousa E: Acute effect of Bauhinia forficata on serum glucose levels in normal and alloxan-induced diabetic rats. Journal of Ethnopharmacology 2002, 83(1–2): 33-37.
• Usman UZ, Abu Bakar AB, Mohamed M: .A Review on experimental methods of diabetic research: Advantages and limitations. Annual Research & Review in Biology 2015, 7(2): 100-108.
• Man ZW, Zhu M, Noma Y, Toide K, Sato T, Asahi Y, Hirashima T, Mori S, Kawano K, Mizuno A, Sano T, Shima K: Impaired beta-cell function and deposition of fat droplets in the pancreas as a consequence of hypertriglyceridemia in OLETF rat, a model of spontaneous NIDDM. Diabetes 1997, 46(11): 1718-1724.
• Yoon J, Austin M, Onodera T, Notkins AL: Virus-induced diabetes mellitus. New England Journal of Medicine 1979, 300(21): 1173-1179.
• Lipes MA, Eisenbarth GS: Transgenic mouse models of type I diabetes. Diabetes 1990, 39(8): 879-884.
• Etuk E: Animal models for studying diabetes mellitus. Agriculture and Biology Journal of North America 2010, 1(2): 130-134.
• Srinivasan K, Ramarao P: Animal models in type 2 diabetes research: an overview. Indian Journal of Medical Research 2007, 125(3): 451-472.
• İrer SV, Alper G: Deneysel diyabet modelleri. Türk Klinik Biyokimya Dergisi 2004. 2(3): 127-136.
• Jun HS, Yoon JW: The role of viruses in type I diabetes: two distinct cellular and molecular pathogenic mechanisms of virus-induced diabetes in animals. Diabetologia 2001, 44(3): 271-285.
• Grover N, Bafna PA, Rana AC: Diabetes and methods to induce experimental diabetes. International Journal of Pharmacy and Biological Sciences 2011, 1(4): 414-419.
• Kumar S, Singh R, Vasudeva N, Sharma S: Acute and chronic animal models for the evaluation of anti-diabetic agents. Cardiovascular Diabetology 2012, 11: 9.
• Bolzan AD, Bianchi MS: Genotoxicity of streptozotocin. Mutation Research 2002, 512(2-3): 121-134.
• Patterson E, Marques TM, O’Sullivan O, Fitzgerald P, Fitzgerald GF, Cotter PD, Dinan TG, Cryan JF, Stanton C, Ross RP: Streptozotocin-induced type-1-diabetes disease onset in Sprague- Dawley rats is associated with an altered intestinal microbiota composition and decreased diversity. Microbiology 2015, 161(1): 182-193.
• Degirmenci I, Kalender S, Ustuner MC, Kalender Y, Gunes HV, Unal N, Basaran A: The effects of acarbose and Rumex patientia on liver ultrastructure in streptozotocin-induced diabetic (type II) rats. Drugs under Experimental and Clinical Research 2002, 28(6): 229-234.
• Shirwaikar A, Rajendran K, Dinesh Kumar C, Bodla R: Antidiabetic activity of aqueous leaf extract of Annona squamosa in streptozotocin-nicotinamide type 2 diabetic rats. Journal of Ethnopharmacology 2004, 91(1): 171-175.
• Sokeng S, Rokeya B, Mostafa M, Nahar N, Mosihuzzaman M, Ali L, Kamtchouing P: Antihyperglycemic effect of bridelia ndellensis ethanol extract and fractions in streptozotocin induced diabetic rats. African Journal of Traditional Complementary and Alternative medicines 2005, 2(2): 94-102.
• Abeeleh MA, Ismail ZB, Alzaben KR, Abu-Halaweh SA, Al-Essa MK, Abuabeeleh J, Alsmady MM: Induction of diabetes mellitus in rats using intraperitoneal streptozotocin: a comparison between 2 strains of rats. European Journal of Scientific Research 2009, 32(3): 398.
• Masiello P, Broca C, Gross R, Roye M, Manteghetti M, Hillaire-Buys D, Novelli M, Ribes G: Experimental NIDDM: development of a new model in adult rats administered streptozotocin and nicotinamide. Diabetes 1998, 47(2): 224-229.
• Akbarzadeh A, Norouzian D, Mehrabi MR, Jamshidi Sh, Farhangi A, Allah Verdi A, Mofidian SMA, Lame Rad B: Induction of diabetes by Streptozotocin in rats. Indian Journal of Clinical Biochemistry 2007, 22(2): 60-64.
• Kamble H, Bodhankar SL: Trigonelline and sitagliptin attenuates nicotinamide-streptozotocin induced diabetic nephropathy in wistar rats. International Journal of Pharmacy and Pharmaceutical Sciences 2013, 5(4): 583-589.
• Qinna NA, Badwan AA: Impact of streptozotocin on altering normal glucose homeostasis during insulin testing in diabetic rats compared to normoglycemic rats. Journal of Drug Design, Development and Theraphy 2015, 9: 2515-2525.
• Pouya P, Sadrkhanlou R, Hasanzadeh S, Foroughi A: An investigation on body weights, blood glucose levels and pituitary-gonadal axis hormones in diabetic and metformin-treated diabetic female rats. Veterinary Research Forum 2012, 3(2): 79-84.
• Carvalho EN, Carvalho NA, Ferreira LM: Experimental model of induction of diabetes mellitus in rats. Acta Cirurgica Brasileira 2003, 18: 60-64.
• Ríos-Silva M, Trujillo X, Trujillo-Hernández B, Sánchez-Pastor E, Urzşa Z, Mancilla E, Huerta M: Effect of chronic administration of forskolin on glycemia and oxidative stress in rats with and without experimental diabetes. International Journal of Medical Sciences 2014, 11(5): 448-452.
• Shirwaikar A, Rajendran K, Barik R: Effect of aqueous bark extract of Garuga pinnata Roxb. in streptozotocin-nicotinamide induced type-II diabetes mellitus. Journal of Ethnopharmacology 2006, 107(2): 285-290.
• Palsamy P, Sivakumar S, Subramanian S: Resveratrol attenuates hyperglycemia-mediated oxidative stress, proinflammatory cytokines and protects hepatocytes ultrastructure in streptozotocin-nicotinamide- induced experimental diabetic rats. Chemico-Biological Interactions 2010, 186(2): 200-202.