Geranium psilostemon Ledeb. bitkisinden elde edilen fenolik bileşikler ve hidrolize olabilen tanenlerin sitotoksik ve genotoksik özelliklerinin değerlendirilmesi

Yıl 2017, Sayı: 2, 63 - 72, 01.06.2017

Suna Sabuncuoğlu Solmaz Mohammadi Nejad Didem Şöhretoğlu

Öz

Geranium türleri, geleneksel tıpta tonik, diüretik, antidiyabetik, antidiyareal ve antihemoroidal olarak kullanılan tıbbi gıda bitkileridir. Geranium psilostemon Ledeb. Geraniaceae , fenolik bileşiklerce zengin, Türkiye’de yaygın olarak yetişen bir türdür. Bu çalışmanın amacı, G. psilostemon’dan elde edilen bileşiklerin sitotoksisitesinin ve genotoksisitesinin değerlendirilmesidir. Bileşiklerin sitotoksisitesi neutral red uptake NRU yöntemi ile belirlenmiştir. Genotoksik etkilerinin değerlendirilmesinde COMET kullanılmıştır. Bileşiklerin IC50 değerleri farklı hücre kültürlerinde sitotoksisitenin değerlendirilmesi için kullanılmıştır. 1,3,6-tri-O-galloil- β-glukopiranoz L1210 ve V79 hücrelerinde en çok sitotoksik etkiyi göstermiştir ve IC50 değerleri sırasıyla 3.7 ve 13 μg/ml’dır. Bunun yanısıra, HeLa hücrelerinde, 1,3,6-tri-O-galloil- β-glukopiranoz ve gallik acit en düşük IC50, sırasıyla 18 and 15 μg/ml değerine sahiptir. Tüm bileşikler kullanıldıkları bütün konsantrasyonlarda sitotoksik etki göstermiştir. Ayrıca, 50 μg/ml konsantrasyonda genotoksik etkileri de gösterilmiştir. G. psilestemon’dan izole edilen test bileşikleri, tıbbi gıda bitkileri olup sitotoksik ve genotoksik potansiyelleri bulunmaktadır. Dolayısıyla, bu biyolojik aktivitelerinin de değerlendirilmesi gerekir. Antikanser ve diğer biyolojik aktivitelerinin değerlendirilebilmesinde optimum konsntrasyonların belirlenmesi için ileri çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

fenolik bileşik, hidrolize olabilen tane, sitotoksisite, genotoksisite

Assesment of Cytotoxic and Genotoxic Properties of Phenolic Compounds and Hydrolysable Tannins from Geranium psilostemon Ledeb.

Öz

Geranium species are medicinal food plants used as tonic, diuretic, antidiabetic, antidiarrheal, and antihemorrhoidal in traditional medicine. Geranium psilostemon Ledeb. Geraniaceae , which has a rich phenolic content, grows widely in Turkey. The aim of this study is to evaluate cytotoxicity and genotoxicity of the compounds isolated from G. psilostemon. Cytotoxic effects of the compounds were determined by neutral red uptake NRU assay. COMET assay was used for assesing genotoxic effects of the compounds. IC50 values of the compounds were calculated in different cell lines to evaluate cytotoxicity. 1,3,6-tri-O-galloyl-β-glucopyranose showed the most cytotoxic effect on L1210 and V79 cell lines and IC50 values of the compound were 3.7 and 13 μg/ml, respectively. Besides, in HeLa cell line, 1,3,6-tri-O-galloyl-β-glucopyranose and gallic acid had the lowest IC50, 18 and 15 μg/ml, respectively. All the compounds exhibited significant cytotoxic effects at all concentrations. Besides, they also showed genotoxic activity at 50 μg/ml. The tested compounds isolated from G. psilestemon, a medicinal food plant, have cytotoxic and genotoxic potential. Therefore, it should be considered regarding these biological acitivities. Further studies are necessary to determine the optimal concentrations of the compounds for evaluating their anticancer and other biological activities.

Anahtar Kelimeler

phenolic compound, hydrolysable tannin, cytotoxicity, genotoxicity

Kaynakça

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