Bazı Doğal Fenolik Bileşiklerin Antioksidan Kapasiteleri ve Farklı Hücre Hatlarında Sitotoksik Etkileri

Yıl 2014, Sayı: 1, 60 - 72, 01.01.2014

Merve Bacanlı Hatice Gül Göktaş Zehra Sarıgöl Sevtap Aydın A. Ahmet Başaran Nurşen Başaran

Öz

Bitkisel fenolik bileşikler insan beslenmesinin önemli bir bileşenidir ve son yıllarda bitkiler ve bitkisel fenolik bileşiklerin yararlı özelliklerine olan ilgi yoğunlaşmıştır. Bu bileşikler antioksidan, antiplatelet, antienflamatuvar ve antikanser etkiler gibi çok çeşitli biyolojik etkiler göstermektedirler. Kurkumin, resveratrol ve rosmarinik asit antioksidan etkileri bilinen fenolik bileşiklerdir ve genellikle kardiyovasküler ve nörodejeneratif hastalıklar gibi oksidatif stres ile ilişkili hastalıkların tedavisinde ve bu hastalıklardan korunmada kullanılmaktadırlar. Bu çalışmada, bu fenolik bileşiklerin antioksidan kapasiteleri troloks eşdeğer antioksidan kapasite yöntemi TEAC ile ve sitotoksisiteleri sağlıklı hücre hattı Çin hamster yumurtalık hücreleri CHO ve tümor hücre hatlarında İnsan meme kanseri hücreleri BT-474 ve insan epitelyal adenokarsinom hücreleri HeLa nötral kırmızı alım yöntemi ile belirlenmiştir. Bu çalışmanın sonucunda, resveratrol ve rosmarinik asitin trolokstan çok daha fazla antioksidan kapasiteye sahip olduğu, kurkuminin ise troloksa göre daha az antioksidan kapasiteye sahip olduğu gösterilmiştir. Sağlıklı CHO hücre hattında, kurkumin, resveratrol, rosmarinik asitin önemli sitotoksik etkileri olduğu görülmüştür. Ancak HeLa ve BT-474 gibi kanser hücrelerinde, bu bileşiklerin farklı özellik göstererek bu hücrelerde sitotoksik olmadıkları ve bu nedenle, bu kanser türlerinde kullanımlarının faydalı olamayacağı görülmüştür.

Anahtar Kelimeler

Kurkumin, Resveratrol, Rosmarinik asit, Sitotoksisite, Troloks eşdeğer antioksidan kapasite testi, Nötral kırmızı alım testi

Antioxidant capacities and cytotoxic properties of some natural phenolic compounds in different cell lines

Öz

Plant phenolic compounds are important constituents of the human diet and in recent years attention has been drawn to beneficial properties of plants and its phenolic compounds. They exhibit a wide range of biological effects, including antioxidant, antiplatelet, anti-inflammatory and anticancer activities. Curcumin, resveratrol and rosmarinic acid are phenolic compounds which are known as antioxidants and commonly used for the preventation and treatment of oxidative stress related diseases such as cardiovascular and neurodegenerative disorders and cancer. In this study, we determined the antioxidant capacities of these phenolic compounds by the trolox equivalent antioxidant capacity TEAC assay and their cytotoxicity by neutral red uptake NRU assay in healthy cell line i.e., chinese hamster ovary CHO , and two tumor cell lines human breast carcinoma BT- 474 and human epithelial adenocarcinoma HeLa . Our results showed that resveratrol and rosmarinic acid have significantly more antioxidant capacity than trolox whereas curcumin showed less antioxidant capacity compared to trolox. In healty CHO cell line, curcumin, resveratrol and rosmarinic acid showed significantly cytotoxic activity, but in cancer cell lines such as HeLa and BT-474, all of the tested compounds have shown different profile. It seems to be not cytotoxic to HeLa and BT-474 cancer cell lines assuming that its usage in these cancer types are not beneficial.

Anahtar Kelimeler

Curcumin, Resveratrol, Rosmarinic acid, Cytotoxicity, Trolox equivalent antioxidant capacity assay, Neutral red uptake assay

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