İnsan Genomik DNA ile Simvastatinin etkileşmesinin incelenmesi

Year 2012, Issue: 1, 1 - 16, 01.01.2012

Mehmet Lütfi Yola Nuran Özaltın

Abstract

Bir hipolipidemik ilaç olan simvastatinin insan genomik DNA ile etkileşmesi dönüşümlü voltametri CV , differansiyel puls voltametrisi DPV ve UV/Vis spektroskopisi yöntemleri kullanılarak incelenmiştir. Simvastatinin insan genomik DNA ile etkileşmesi sonucunda simvastatinin pik akımında önemli bir azalmayla birlikte maksimum absorpsiyon bandlarında hipokromik ve batokromik etki gözlenmiştir. Simvastatinin elektrokimyasal ve spektral özelliklerindeki değişiklikler DNA ile interkalasyon bir şekilde etkileştiğini belirtmektedir. Voltametrik ve spektroskopik veriler kullanılarak bağlanma sabitleri tayin edilmiştir. Ayrıca etkileşme üzerine iyonik şiddetinin etkisi simvastatinin DNA çift sarmalına interkalasyonunu desteklemektedir. Bu çalışmalar ilacın tedavi edici etkisinin belirlenmesi açısından önemli olan simvastatin – DNA etkileşiminin daha iyi anlaşılması ve ilerde yeni DNA hedefli ilaçların tasarlanması açısından değerlidir.

Keywords

Simvastatin, İnsan Genomik DNA, Elektrokimya, UV/Vis spektroskopisi

Investigation of Interaction of Simvastatin with Human Genomic DNA

Abstract

The interaction of simvastatin SIM , a hipolipidemic drug, with human genomic DNA has been investigated by cyclic voltammetry CV , differential pulse voltammetry DPV and UV/Vis spectroscopy. As a result of the interaction of SIM with human genomic DNA, a considerable decrease in the SIM peak currents and a hypochromic and bathochromic shift in the maximum adsorption bands of SIM was observed. The changes in the electrochemical and spectral characteristics of SIM indicated SIM bind to DNA by intercalative mode. Binding constants were determined using voltammetric and spectroscopic data. In addition that the effect of ionic strength on the interaction support the intercalation of SIM into the DNA double helix. These studies are valuable for a better understanding SIM – DNA interaction, which should be important into the determination of therapeutic efficacy of the drug and design of new DNA targeted drug in future.

Keywords

Simvastatin, Human genomic DNA, Electrochemistry, UV/Vis spectroscopy

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