Sürfaktin İçeren Kendiliğinden Mikroemülsifiye Olabilen İlaç Taşıyıcı Sistemlerin Formülasyonu ve Karakterizasyonu
Abstract
Bu projenin amacı; çok güçlü bir biyosürfaktan ve aynı zamanda çeşitli terapötik etkileri olan sürfaktin SF ’in, kendiliğinden mikroemülsifiye olabilen sistemler SMEDDS içinde formüle edilmesidir. Bunun için, üçgen faz diyagramları kullanılarak geliştirilen SMEDDS formülasyonları hazırlanmış ve karakterizasyon çalışmaları yürütülmüştür. Fiziksel görünüm, pH, damlacık büyüklüğü, zeta potansiyel, termal ve mikroskopik incelemeler; dayanıklı ve teknolojik açıdan uygun SF-SMEDDS elde edildiğini göstermiştir. Bilindiği kadarıyla, bu çalışma SF’in şimdiye kadar SMEDDS gibi bir farmasötik dozaj formunda formülasyonunu gösteren ilk çalışmadır.
Keywords
Sürfaktin kendiliğinden- mikro/nano emülsifiye olabilen ilaç taşıyıcı sistemler biyosürfaktanlar
References
- Das P, Mukherjee S and Sen R: Antimicrobial potential of a lipopeptide biosurfactant derived from a marine Bacillus circulans. J Appl Microbiol 104: 1675-84, 2008.
- Kim KM, Lee JY, Kim CK and Kang JS: Isolation and characterization of surfactin produced by Bacillus polyfermenticus KJS-2. Arch Pharm Res 32: 711-5, 2009.
- Carrillo C, Teruel JA, Aranda FJ and Ortiz A: Molecular mechanism of membrane permeabilization by the peptide antibiotic surfactin. Biochim Biophys Acta 1611: 91-7, 2003.
- Hsieh FC, Li MC, Lin TC and Kao SS: Rapid detection and characterization of sur- factin-producing Bacillus subtilis and closely related species based on PCR. Curr Microbiol 49: 186-91, 2004.
- Kim PI, Bai H, Bai D, Chae H, Chung S, Kim Y, Park R and Chi YT: Purification and characterization of a lipopeptide produced by Bacillus thuringiensis CMB26. J Appl Microbiol 97: 942-9, 2004.
- Pouton CW: Formulation of self-emulsifying drug delivery systems. Adv Drug Deliver Rev 25: 47-58, 1997.
- Gursoy RN and Benita S: Self-emulsifying drug delivery systems (SEDDS) for im- proved oral delivery of lipophilic drugs. Biomed Pharmacother 58: 173-82, 2004.
- Gursoy N, Garrigue JS, Razafindratsita A, Lambert G and Benita S: Excipient effects on in vitro cytotoxicity of a novel paclitaxel self-emulsifying drug delivery system. J Pharm Sci 92: 2411-2418, 2003.
- Tang J, Sun, J., He, Z.: Self-Emulsifying Drug Delivery Systems: Strategy For Improv- ing Oral Delivery Of Poorly Soluble Drugs. Curr Drug Ther 2: 85-93, 2007.
- Yang S, Gursoy RN, Lambert G and Benita S: Enhanced oral absorption of paclitaxel in a novel self-microemulsifying drug delivery system with or without concomitant use of P-glycoprotein inhibitors. Pharm Res 21: 261-70, 2004.
- Seydlova G and Svobodova J: Review of surfactin chemical properties and the poten- tial biomedical applications. Cent Eur J Med 3: 123-133, 2008.
- Oka K, Hirano T, Homma M, Ishii H, Murakami K, Mogami S, Motizuki A, Morita H, Takeya K and Itokawa H: Satisfactory separation and MS-MS spectrometry of six surfactins isolated from Bacillus subtilis natto. Chem Pharm Bull (Tokyo) 41: 1000-2, 1993.
- Abdel-Mawgoud AM, Aboulwafa MM and Hassouna NA: Characterization of surfactin produced by Bacillus subtilis isolate BS5. Appl Biochem Biotechnol 150: 289-303, 2008.
- Kim HS, Yoon BD, Lee CH, Suh HH, Oh HM, Katsuragi T and Tani Y: Production and properties of a lipopeptide biosurfactant from Bacillus subtilis C9. J Ferment Bioeng 84: 41-46, 1997.
- Wei YH and Chu IM: Enhancement of surfactin production in iron-enriched media by Bacillus subtilis ATCC 21332. Enzyme Microb Tech 22: 724-728, 1998.
- Gershanik T, Haltner E, Lehr CM and Benita S: Charge-dependent interaction of self- emulsifying oil formulations with Caco-2 cells monolayers: binding, effects on barrier function and cytotoxicity. Int J Pharm 211: 29-36, 2000.
- Peira E, Carlotti ME, Trotta C, Cavalli R and Trotta M: Positively charged microemul- sions for topical application. Int J Pharm 346: 119-23, 2008.
Formulation and Characterization of Surfactin-Containing Self- Microemulsifying Drug Delivery Systems SF-SMEDDS
Abstract
The aim of this project was to formulate surfactin SF , a powerful biosurfactant with several therapeutic properties, into self-microemulsifying drug delivery systems SMEDDS . Thus, SMEDDS formulations were developed and prepared utilizing ternary phase diagrams, and characterization studies were carried out. The physical appearance, pH, droplet size, zeta potential, thermal and microscopic properties indicate that stable and technologically suitable SF-SMEDDS were obtained. To the best of our knowledge, this is the first study showing the successful incorporation of SF into a pharmaceutical dosage form such as SMEDDS.
References
- Das P, Mukherjee S and Sen R: Antimicrobial potential of a lipopeptide biosurfactant derived from a marine Bacillus circulans. J Appl Microbiol 104: 1675-84, 2008.
- Kim KM, Lee JY, Kim CK and Kang JS: Isolation and characterization of surfactin produced by Bacillus polyfermenticus KJS-2. Arch Pharm Res 32: 711-5, 2009.
- Carrillo C, Teruel JA, Aranda FJ and Ortiz A: Molecular mechanism of membrane permeabilization by the peptide antibiotic surfactin. Biochim Biophys Acta 1611: 91-7, 2003.
- Hsieh FC, Li MC, Lin TC and Kao SS: Rapid detection and characterization of sur- factin-producing Bacillus subtilis and closely related species based on PCR. Curr Microbiol 49: 186-91, 2004.
- Kim PI, Bai H, Bai D, Chae H, Chung S, Kim Y, Park R and Chi YT: Purification and characterization of a lipopeptide produced by Bacillus thuringiensis CMB26. J Appl Microbiol 97: 942-9, 2004.
- Pouton CW: Formulation of self-emulsifying drug delivery systems. Adv Drug Deliver Rev 25: 47-58, 1997.
- Gursoy RN and Benita S: Self-emulsifying drug delivery systems (SEDDS) for im- proved oral delivery of lipophilic drugs. Biomed Pharmacother 58: 173-82, 2004.
- Gursoy N, Garrigue JS, Razafindratsita A, Lambert G and Benita S: Excipient effects on in vitro cytotoxicity of a novel paclitaxel self-emulsifying drug delivery system. J Pharm Sci 92: 2411-2418, 2003.
- Tang J, Sun, J., He, Z.: Self-Emulsifying Drug Delivery Systems: Strategy For Improv- ing Oral Delivery Of Poorly Soluble Drugs. Curr Drug Ther 2: 85-93, 2007.
- Yang S, Gursoy RN, Lambert G and Benita S: Enhanced oral absorption of paclitaxel in a novel self-microemulsifying drug delivery system with or without concomitant use of P-glycoprotein inhibitors. Pharm Res 21: 261-70, 2004.
- Seydlova G and Svobodova J: Review of surfactin chemical properties and the poten- tial biomedical applications. Cent Eur J Med 3: 123-133, 2008.
- Oka K, Hirano T, Homma M, Ishii H, Murakami K, Mogami S, Motizuki A, Morita H, Takeya K and Itokawa H: Satisfactory separation and MS-MS spectrometry of six surfactins isolated from Bacillus subtilis natto. Chem Pharm Bull (Tokyo) 41: 1000-2, 1993.
- Abdel-Mawgoud AM, Aboulwafa MM and Hassouna NA: Characterization of surfactin produced by Bacillus subtilis isolate BS5. Appl Biochem Biotechnol 150: 289-303, 2008.
- Kim HS, Yoon BD, Lee CH, Suh HH, Oh HM, Katsuragi T and Tani Y: Production and properties of a lipopeptide biosurfactant from Bacillus subtilis C9. J Ferment Bioeng 84: 41-46, 1997.
- Wei YH and Chu IM: Enhancement of surfactin production in iron-enriched media by Bacillus subtilis ATCC 21332. Enzyme Microb Tech 22: 724-728, 1998.
- Gershanik T, Haltner E, Lehr CM and Benita S: Charge-dependent interaction of self- emulsifying oil formulations with Caco-2 cells monolayers: binding, effects on barrier function and cytotoxicity. Int J Pharm 211: 29-36, 2000.
- Peira E, Carlotti ME, Trotta C, Cavalli R and Trotta M: Positively charged microemul- sions for topical application. Int J Pharm 346: 119-23, 2008.
Details
| Primary Language | English |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | June 1, 2010 |
| Published in Issue | Year 2010 Issue: 2 |