V79 Hücre Hattında ve İnsan Lenfositlerinde Öjenolün Antioksidant Aktivitesinin, Sitotoksisitesinin ve Genotoksisitesinin Değerlendirmesi

Year 2019, Volume: 39 Issue: 1, 1 - 9, 15.01.2019

Solmaz M. Nejad Hilal Özgüneş Nurşen Başaran

Abstract

Öjenol, Eugenia Caryophyllata’nın yaprak ve tomurcuklarından elde edilen, ka- ranfil yağının fenolik yapıdaki uçucu bir bileşenidir. İlaç, kozmetik, tarım ve gıda endüstrisinde kullanılmaktadır. Türevlerin lokal antiseptik ve anestezik olarak tıpta kullanımı bulunmaktadır. Öjenol genel olarak güvenli bir bileşik olarak de- ğerlendirilir ancak çok farklı uygulamaları ve yaygın kullanım alanları nedeniyle, toksisitesi son yıllarda ilgi odağı olmuştur. Öjenol’ün sitotoksisitesi ve genotok- sisitesi ile ilgili çalışmalar yetersiz ve çelişkilidir.
Bu çalışmada, öjenolün V79 hücre hattında in vitro antioksidan aktivitesi ve si- totoksisitesi TEAC test ve nötral kırmızı alım yöntemiyle incelenmiştir. Sonuçla- ra göre, öjenol’un doza bağımlı olarak V79 hücrelerinde antioksidan aktivite ve sitotoksik etkiye sahip olduğu gösterilmiştir.Öjenol’un IC50 dozunun 341.5 μM olduğu bulunmuştur. Ayrıca, öjenol’un in vitro genotoksik etkileri, tek hücre jel elektroforez (Comet yöntemi) ve mikroçekirdek yön- temi ile insan periferal lenfositleri üzerinde incelennmiş- tir. Sonuçlarımıza göre, EUG negatif kontrol hücreleriyle karşılaştırıldığında, daha yüksek konsantrasyonlarda DNA hasarı yaratma potansiyeline sahiptir. Ayrıca bu çalışmada, a öjenol’un hücreleri H2O2 kaynaklı oksidatif DNA hasa- rına karşı koruduğunu göstermektedir.

Keywords

Öjenol, Genotoksisite, Sitotoksisite, Tek hücre jel elektroforez (Comet) yöntemi, mikroçekirdek yöntemi.

Evaluation of Antioxidant Activity, Cytotoxicity and Genotoxicity of Eugenol in V79 cell line and Human Lymphocytes Respectively

Abstract

Eugenol (EUG) is a volatile phenolic constituent of clove essential oil obtained from Eugenia Caryophyllata buds and leaves. It has been used in pharmaceuti- cal, cosmetic, agriculture and food industry. The derivatives have been used in medicine as a local antiseptic and anesthetic. Although EUG is considered safe as a food additive, due to the vast range of different applications and extensive use, there has been a great concern about its toxicity in recent years. However, studies about cytotoxicity and genotoxicity of EUG are very limited.
In the present study, we investigated the in vitro antioxidant activity and the cy- totoxicity of EUG on V79 cell line by the TEAC Assay and Neutral Red Uptake Assay (NRU). Our results demonstrated that EUG has antioxidant activity and cytotoxic effect in V79 cell line in a dose dependent manner. The IC50 value of
EUG in V79 cell line was found to be 341.5 μM.
The in vitro genotoxic effects of EUG are also studied on human peripheral lym- phocytes by Single Cell Gel Electrophoresis (Comet) assay and micronucleus assay. Results show that EUG is potent to induce DNA damage at higher con- centrations when compared with untreated control cells. Our study also indicated that EUG is potent to protect cells against H2O2-induced oxidative DNA damage.

Keywords

Eugenol (EUG), Cytotoxicity, Genotoxicity, Single Gel Electrophoresis (Comet) Assay, Cytokinesis-block Micronucleus (CBMN) Assay

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