Identification of causative species in patients with cuteneous leishmaniasis in Diyarbakır by polymerase chain reaction (pcr)-restriction fragment lenght polymorphism (RFLP)

Year 2014, Volume: 6 Issue: 2, 17 - 20, 01.12.2014

Nezahat Akpolat İbrahim Halil Yıldırım Mutalip Çiçek Şebnem Nergiz Özgür Ezin Nida Özcan Mehmet Harman

Abstract

Background: Leishmaniasis is a protozoan disease caused by more than twenty Leishmania species which
are transmitted by infected phlebotomine sandflies to humans. Leishmaniasis include multiple clinical
syndromes such as visceral , mucosal and cutaneous forms. Cutaneous Leishmaniasis (CL) is the most
common and endemic form in southeastern Turkey.
Purpose: CL can be caused by L. major, L. tropica, L. mexicana and L. amazonensis. Microscopy remains to
be the standard diagnostic method because of its cheapness, ease of application and high
specificity . Microscopic examination of smears, parasite culture and serological tests are performed for
diagnosis. Recently several molecular methods, especially those based on the Polymerase chain reaction
(PCR) have been developed for identification of Leishmania species.
Method: In this study, 150 smear samples taken from patients with clinical findings of CL were studied by
PCR- RFLP (restriction fragment length polymorphism)
Results: Leishmania tropica was detected in 128 smear sample. L. tropica was found to be the most common
species causing CL in southeastern Turkey.
Conclusion: Identification of Leishmania species is important because different types may require different
treatments. Determining the common species in the region may lead to develop treatment protocols.

Keywords

Cutaneous Leishmaniasis, Polymerase Chain Reaction (PCR), Restriction Fragment Lenght Polymorphısm (RFLP)

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