Yıl 2018, Cilt 10 , Sayı 2, Sayfalar 33 - 39 2018-12-07

GeneXpert vanA/vanB test and culture method in evaluation of Vancomycin Resistant Enterococcus (VRE) colonisation in a tertiary hospital

Nida ÖZCAN [1] , Nezahat AKPOLAT [2] , Selahattin ATMACA [3] , Kadri GÜL [4]


Background: Enterococci are responsible for various nosocomial infections. The spread of vancomycin-resistant enterococci (VRE) especially in intensive care units (ICU) is a major threat in hospitals. Determination of VRE colonisers is crucial in order to prevent spreading of VRE. It was aimed to determine the colonisation of VRE among ICU patients by GeneXpert vanA/vanB test and culture method in a period of two years.
Method: From April 2015 to March 2017, a total of 788 rectal swab specimens were obtained from 292 ICU patients hospitalized at Dicle University hospitals. Swab samples were evaluated for VanA and VanB genes by GeneXpert® vanA / vanB, (Cepheid, USA) real time polymerase chain reaction (Rt-PCR) commercial system. Enterococcosel agar with 8 μg/mL vancomycin was used for cultivation of the samples. Swab samples were inoculated at 37°C, in aerobic condition for 48 hours. The growing bacteria were identified by Maldi Biotyper (Bruker, Germany) and antimicrobial susceptibilities were carried by BD Phoenix (Becton Dickinson, U.S.A) authomated microbiology system.
Results:Among 788 swab samples 116 (14,7%) were detected as VRE with GeneXpert vanA/vanB method. Of PCR positive samples, 107 (92,2%) were VanA, 7 (6%) were VanB and 2 (1,7%) were both VanA and VanB harbouring. Among VanA detected samples, 75 grew Enterococcus faecium, 8 grew Enterococcus faecalis isolates while 24 samples had no growth in cultivation. Among 7 VanB harbouring samples, 1 was identified as E. faecium while other 6 had no growth.Two samples with both VanA and VanB genes were isolated as E. faecium.
Conclusion: Active VRE surveillance and isolation of VRE carriers is crucial in hospitals. Molecular methods have important advantages in early diagnosis and on time isolation of the patients.

vancomycin-resistant enterococci, vanA / vanB
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Birincil Dil en
Konular Genel ve Dahili Tıp
Bölüm Araştırma
Yazarlar

Yazar: Nida ÖZCAN (Sorumlu Yazar)

Yazar: Nezahat AKPOLAT

Yazar: Selahattin ATMACA

Yazar: Kadri GÜL

Tarihler

Yayımlanma Tarihi : 7 Aralık 2018

Bibtex @araştırma makalesi { iamr511671, journal = {International Archives of Medical Research}, issn = {2146-6033}, address = {Dicle Üniversitesi Tıp Fakültesi Histoloji ve Embriyoloji AD, 21280, Diyarbakır-Türkiye}, publisher = {Veysi AKPOLAT}, year = {2018}, volume = {10}, pages = {33 - 39}, doi = {}, title = {GeneXpert vanA/vanB test and culture method in evaluation of Vancomycin Resistant Enterococcus (VRE) colonisation in a tertiary hospital}, key = {cite}, author = {ÖZCAN, Nida and AKPOLAT, Nezahat and ATMACA, Selahattin and GÜL, Kadri} }
APA ÖZCAN, N , AKPOLAT, N , ATMACA, S , GÜL, K . (2018). GeneXpert vanA/vanB test and culture method in evaluation of Vancomycin Resistant Enterococcus (VRE) colonisation in a tertiary hospital. International Archives of Medical Research , 10 (2) , 33-39 . Retrieved from https://dergipark.org.tr/tr/pub/iamr/issue/42467/511671
MLA ÖZCAN, N , AKPOLAT, N , ATMACA, S , GÜL, K . "GeneXpert vanA/vanB test and culture method in evaluation of Vancomycin Resistant Enterococcus (VRE) colonisation in a tertiary hospital". International Archives of Medical Research 10 (2018 ): 33-39 <https://dergipark.org.tr/tr/pub/iamr/issue/42467/511671>
Chicago ÖZCAN, N , AKPOLAT, N , ATMACA, S , GÜL, K . "GeneXpert vanA/vanB test and culture method in evaluation of Vancomycin Resistant Enterococcus (VRE) colonisation in a tertiary hospital". International Archives of Medical Research 10 (2018 ): 33-39
RIS TY - JOUR T1 - GeneXpert vanA/vanB test and culture method in evaluation of Vancomycin Resistant Enterococcus (VRE) colonisation in a tertiary hospital AU - Nida ÖZCAN , Nezahat AKPOLAT , Selahattin ATMACA , Kadri GÜL Y1 - 2018 PY - 2018 N1 - DO - T2 - International Archives of Medical Research JF - Journal JO - JOR SP - 33 EP - 39 VL - 10 IS - 2 SN - 2146-6033- M3 - UR - Y2 - 2020 ER -
EndNote %0 International Archives of Medical Research GeneXpert vanA/vanB test and culture method in evaluation of Vancomycin Resistant Enterococcus (VRE) colonisation in a tertiary hospital %A Nida ÖZCAN , Nezahat AKPOLAT , Selahattin ATMACA , Kadri GÜL %T GeneXpert vanA/vanB test and culture method in evaluation of Vancomycin Resistant Enterococcus (VRE) colonisation in a tertiary hospital %D 2018 %J International Archives of Medical Research %P 2146-6033- %V 10 %N 2 %R %U
ISNAD ÖZCAN, Nida , AKPOLAT, Nezahat , ATMACA, Selahattin , GÜL, Kadri . "GeneXpert vanA/vanB test and culture method in evaluation of Vancomycin Resistant Enterococcus (VRE) colonisation in a tertiary hospital". International Archives of Medical Research 10 / 2 (Aralık 2018): 33-39 .
AMA ÖZCAN N , AKPOLAT N , ATMACA S , GÜL K . GeneXpert vanA/vanB test and culture method in evaluation of Vancomycin Resistant Enterococcus (VRE) colonisation in a tertiary hospital. IAMR. 2018; 10(2): 33-39.
Vancouver ÖZCAN N , AKPOLAT N , ATMACA S , GÜL K . GeneXpert vanA/vanB test and culture method in evaluation of Vancomycin Resistant Enterococcus (VRE) colonisation in a tertiary hospital. International Archives of Medical Research. 2018; 10(2): 39-33.