Electrophoretic characterization of inbred maize lines

Year 2019, Volume: 3 Issue: 2, 86 - 92, 15.08.2019

Gülsemin Savaş Tuna , Burak Uyanık Elif Eymen Özdemir Görkem Dalgıç Yaren Mengi Kayıhan Z. Korkut

https://doi.org/10.35860/iarej.413379

Abstract

In this study, 50 inbred lines
(S₄) of maize (Zea
mays indentata Sturt.), which were developed by the Department of Field Crops,
Faculty of Agriculture, University of Namık Kemal, were used as a
material.  In the study, the band
patterns of the gliadin protein of inbred lines were determined by the SDS –
PAGE method, and regarding the ratio density data of the genotypes, the number
of the bands and the spreading of them to the gliadin regions were examined. At
the end of the electrophoresis examinations, it was revealed that the band
number of the gliadin proteins in the inbred maize lines was between 11 and 20,
the relative mobility of the genotypes was between 18 and 90 kDA, and according
to the gliadin regions, the bands were mainly in the omega, beta and gamma
regions, respectively. It was found out that the relative mobility was minimum
in the alpha region. As a result of the study, it was determined that some
lines were formed by similar populations with the obtained band patterns, and
the majority were different. This indicates that genetic diversity exists in
the examined lines, and the obtained data can be used in the breeding studies.

Keywords

Zea mays L., Inbred line, SDS-PAGE, Gliadin electrophoresis, Electrophoretic character

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