Radicut'ın Sıçan Dalağındaki Valproik Asite Bağlı Oksidatif Streste Potansiyel Koruyucu Rolü

Yıl 2024, Sayı: 22, 308 - 319, 30.04.2024

Nihal Şehkar Oktay , Burcin Alev Tüzüner , Sevim Tunalı , Ebru Emekli Alturfan , Tuğba Tunalı-akbay , Refiye Yanardağ , Ayşen Yarat

https://doi.org/10.38079/igusabder.1348285

Öz

Amaç: Çalışma, sıçanların dalak dokularında Valproik asitin (VPA) neden olduğu oksidatif streste Radikutun (RAD) potansiyel koruyucu rolünü değerlendirmeyi amaçlamaktadır.
Yöntem: Sıçanlar belirtildiği gibi gruplara ayrılmıştır: Grup 1: Kontrol (n=8), Grup II: R: RAD verilen grup (30 mg/kg/gün, n=8), Grup III: V: VPA verilen grup (0,5 g/kg/gün, n=10), Grup IV: V+R: VPA+ RAD verilen grup (30 mg/kg/gün, n=11). Hayvanlara 7 gün süreyle (i.p) VPA, RAD ve VPA+RAD verildi. Oksidatif stresle ilgili biyokimyasal parametreler dalak homojenatlarında tayin edildi.
Bulgular: VPA, dalakta lipid peroksidasyon ve siyalik asit seviyelerini yükselterek, alkalen fosfataz aktivitesini artırarak ve süperoksit dismutaz, glutatyon s transferaz ve glutatyon peroxidaz aktivitelerini azaltarak oksidatif stresi yükseltmiştir. VPA verilen gruba RAD uygulanması, LPO, SA ve asit fosfataz düzeylerini azaltırken, doku faktörü, SOD, GST ve GPx aktivitelerini artırdı.
Sonuç: RAD, koruyucu etkisini göstererek VPA verilen gruptaki biyokimyasal sonuçları normale çevirdi. RAD, VPA tedavisi sırasında yararlı olabilecek oksidatif stresi önleme potansiyeline sahiptir.

Anahtar Kelimeler

Radikut, valproik asit, oksidatif stres, antioksidanlar, dalak, sıçan

Potential Protective Role of Radicut in Valproic Acid-Induced Oxidative Stress in Rat Spleen

Öz

Aim: The study aims to evaluate the potential protective role of Radicut (RAD) in Valproic acid (VPA)-induced oxidative stress in splenic tissues of rats.
Method: Rats were divided into groups as follows: Group 1: Controls (n=8), Group II: R: RAD-given group (30 mg/kg/day, n=8), Group III: V: VPA-given group (0.5 g/kg/day, n=10), Group IV: V+R: VPA+RAD-given group (30 mg/kg/day, n=11). VPA, RAD, and VPA+RAD were given to the animals for 7 days (i.p). Biochemical parameters related to oxidative stress were determined in spleen homogenates.
Results: VPA elevated oxidative stress by increasing lipid peroxidation and sialic acid levels, increasing alkaline phosphatase activity, and decreasing superoxide dismutase, glutathione-S- transferase, and glutathione peroxidase activities. Administration of RAD to VPA-given group decreased LPO, SA levels, and acid phosphatase levels, and increased tissue factor, SOD, GST, and GPx activities.
Conclusion: RAD reversed the biochemical results in the V group, by clarifying its protective effect. RAD has the potential to prevent oxidative stress during VPA treatment, which could be beneficial.

Anahtar Kelimeler

Radicut, valproic acid, oxidative stress, antioxidants, spleen, rat

Kaynakça

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