Identification, Genetic Diversity and Biological Control of Dollar Spot Disease Caused by Sclerotinia homoeocarpa on Golf Courses in Turkey

Abstract

Dollar spot caused by Sclerotinia homoeocarpa, can create damage to warm and cool season turfgrasses, is a significant disease. In the study, 13 golf courses in 5 provinces of Turkey were surveyed for dollar spot disease caused by Sclerotinia homoeocarpa. During the survey samples were taken from leaves with cream spots, framed by a reddish brown edge and from areas of light yellow small patches. Identifications of Sclerotinia isolates were performed by DNA sequencing analysis. Five bacterial strains were examined to detect their antifungal influences against the dollar spot by using seed coating method in greenhouse conditions. As a result of isolations from infected plants from 13 golf courses, 7 Sclerotinia homoeocarpa (Sh) isolates were obtained. Pathogenicity tests performed in the greenhouse ranged from 81.03% to 90.75%. Consequense of biologic control studies, Pseudonomas putida 88cfp, Pseudomonas putida 166fp and Bacillus cereus 44bac were found efficient the ratio of 92.99%, 88.71% and 87.50% respectively. In further studies, effective bacterial strains (88cfp, 166fp and 44bac) should be carried out in large golf courses.

Keywords

• Sclerotinia homoeocarpa
• turfgrass
• virulence
• biological control

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