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Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber

Yıl 2021, Cilt: 5 Sayı: 1, 106 - 112, 30.06.2021

Öz

This study aimed to: (1) isolate entomopathogenic fungi from infected host insects; (2) conduct bioassay of different entomopathogenic fungi (EPF) isolates against the cucurbit leafworm, Diaphania indica.; and (3) identify the most potential EPF isolate using morphological and molecular characterization. Four entomopathogenic fungi isolates that showed positive infection to D. indica larvae were evaluated. The most virulent isolate was subjected to morphological and molecular characterization. Results showed that EPF Isolate 1 is highly virulent to D. indica larvae with 97.5-100% mortality in the 2nd to 4th instar larvae. EPF Isolate 1 was first to show infection 1.38±0.41 days after inoculation, and larvae were entirely covered with white mycelia 5.25±0.41 days. Molecular identification revealed the isolate as Isaria fumosorosea as indicated by molecular marker internal transcribed space region of 5.8s (ITS-5.8s) ribosomal DNA. EPF Isolate 1 was identified as Isaria fomosorosea. It is the most virulent and potential isolate against cucurbit leafworm, D. indica.

Destekleyen Kurum

Nueva Vizcaya State University

Proje Numarası

none

Teşekkür

Thanks to College of Agriculture, Nueva Vizcaya State University.

Kaynakça

  • Yazdi, F. K., Ezlenberg, J. and Mohammadipour, A. 2011. Biological Characterization of Beauveria bassiana (Clavicipitaceae: Hypocreales) from Overwintering Sites of Sunn Pest, Eurygaster integriceps (Scutelleridae: Heteroptera) in Iran. International Journal of Agricultural Science and Research, 2(2): 1-12.
  • Zimmermann, G. 2008. The entomopathogenic fungi Isaria farinose (formerly Paecilomyces farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus): biology, ecology and use in biological control. Biocontrol Science and Technology 18 (9): 865-901.
Yıl 2021, Cilt: 5 Sayı: 1, 106 - 112, 30.06.2021

Öz

Proje Numarası

none

Kaynakça

  • Yazdi, F. K., Ezlenberg, J. and Mohammadipour, A. 2011. Biological Characterization of Beauveria bassiana (Clavicipitaceae: Hypocreales) from Overwintering Sites of Sunn Pest, Eurygaster integriceps (Scutelleridae: Heteroptera) in Iran. International Journal of Agricultural Science and Research, 2(2): 1-12.
  • Zimmermann, G. 2008. The entomopathogenic fungi Isaria farinose (formerly Paecilomyces farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus): biology, ecology and use in biological control. Biocontrol Science and Technology 18 (9): 865-901.
Toplam 2 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Bitki Bilimi
Bölüm Original Papers
Yazarlar

Ferdinand Arcala 0000-0001-7347-1506

Proje Numarası none
Yayımlanma Tarihi 30 Haziran 2021
Gönderilme Tarihi 17 Aralık 2020
Kabul Tarihi 8 Haziran 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 5 Sayı: 1

Kaynak Göster

APA Arcala, F. (2021). Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber. International Journal of Agriculture Forestry and Life Sciences, 5(1), 106-112.
AMA Arcala F. Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber. Int J Agric For Life Sci. Haziran 2021;5(1):106-112.
Chicago Arcala, Ferdinand. “Morphological and Molecular Characterization of Fungal Entomopathogen Isaria Fumosorosea (Wize) Brown and Smith As Bioinsecticide Against Cucurbit Leafworm, Diaphania Indica Saunders, in Cucumber”. International Journal of Agriculture Forestry and Life Sciences 5, sy. 1 (Haziran 2021): 106-12.
EndNote Arcala F (01 Haziran 2021) Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber. International Journal of Agriculture Forestry and Life Sciences 5 1 106–112.
IEEE F. Arcala, “Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber”, Int J Agric For Life Sci, c. 5, sy. 1, ss. 106–112, 2021.
ISNAD Arcala, Ferdinand. “Morphological and Molecular Characterization of Fungal Entomopathogen Isaria Fumosorosea (Wize) Brown and Smith As Bioinsecticide Against Cucurbit Leafworm, Diaphania Indica Saunders, in Cucumber”. International Journal of Agriculture Forestry and Life Sciences 5/1 (Haziran 2021), 106-112.
JAMA Arcala F. Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber. Int J Agric For Life Sci. 2021;5:106–112.
MLA Arcala, Ferdinand. “Morphological and Molecular Characterization of Fungal Entomopathogen Isaria Fumosorosea (Wize) Brown and Smith As Bioinsecticide Against Cucurbit Leafworm, Diaphania Indica Saunders, in Cucumber”. International Journal of Agriculture Forestry and Life Sciences, c. 5, sy. 1, 2021, ss. 106-12.
Vancouver Arcala F. Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber. Int J Agric For Life Sci. 2021;5(1):106-12.

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