Chronic Unpredictable Mild Stress Affects Weight Changes Through Circadian Cycle Mechanism

Yıl 2024, Cilt: 7 Sayı: 2, 469 - 474, 25.03.2024

Alifia Candra Puriastuti Margarita Maria Maramis Jimmy Yanuar Annas Reny I'tishom Purwo Sri Rejeki Sulistiawati Sulistiawati

https://doi.org/10.33438/ijdshs.1376508

Öz

Everyday stress is unpredictable and comes in many different forms. The person is constantly exposed to stress because they cannot handle it. Chronic stress results from this illness. Chronic stress can affect changes in the body's standard mechanisms, including body weight modulated by the circadian cycle. Due to the fact that each person's response to stress is unique, stressful situations might result in two different variations in body weight. The purpose of this study is tobserve the effect of the CUMS on weight changes in Rattus novergicus through the circadian cycle mechanism. 16 chronic stress models of the Rattus norergicus were split into control and treatment groups. The chronic stress was provided using the Chronic Unpredictable Mild Stress (CUMS) approach. The control group did not receive any treatment but was still given maintenance such as providing food, drink and measuring their body weight every day. While the treatment group received CUMs for 20 days. Every morning before the stressor exposure, body weight was recorded. And the measurement of corticosterone levels using the Enzyme-Linked Immunoassay (ELISA) method with the Duplo technique. Before termination, blood is taken at the heart. Corticosterone levels varied across the groups (p = 0.032). The study's changes in body weight (p = 0.00) revealed a difference between the control and treatment groups, with the treatment group's weight being -11.15 ± 7.91. Chronic stress can alter how the body normally functions, mainly how the circadian cycle regulates body weight. Since each person's response to stress is unique, stressful situations might result in two different variations in body weight.

Anahtar Kelimeler

Chronic Unpredictable Mild Stress, Circadian Cyle, Weight Changes

Kaynakça

• Bohus, B., Korte, S.M., Buwalda, B., Meijer O., Kloet, E.R.D. 1995. Socially Depetaed Male Rats Displays A Blunted Adenocortical Response To A Low Dose of 8-OH-DPAT. European Journal of Pharmacology. 272 : 55 – 56. [Pubmed]
• Brouček, J. Effect of Noice on Performance, Stress, And Behaviour of Animal. Slovak Journal Animal Science. 2014 (2) : 111-123.
• Guyton, Ermita I. Ibrahim Ilyas editorial. (2016). Guyton dan Hall : Buku Ajar Fisiologi Kedokteran Edisi Revisi Berwarna Ke-12. ISBN : 978-981-437-118-6. [Crossref]
• Harding, J.L., Backholer K., Williams E.D., Peeters A., Cameron A.J., Hare M.J., Shaw J.E, Magliano D.J. 2014. Psychosocial Stress is Positively Associated With Body Mass Index Gain Over 5 Years : Evidence From The Longitudinal AusDiab Study. PubMed. 22 (1) : 277-286. [Pubmed]
• Harris, Ruth B.S. 2015. Chronic and Acute Effects of Stress on Energy Balance : are there appropriate animal models?. American Journal od Physiology : Regulatory, Integrative and Comparative Physiology. 308 (4) : 250 – 265. [Pubmed]
• Koch, C.E, B. Leinweber, B.C Drengberg, C. Blaum and H. Oster. 201. Interaction Between Circadian Rhythms and Stress. Neurobiol Stress 6 : 57 – 67. [Pubmed]
• Kusumawati, Diah 2016. Bersahabat dengan Hewan Coba Edisi Pertama. Yogyakarta : Universitas Gajah Mada ISBN : 978-979-420-554-0.
• López-López, A. L., Herlinda Bonilla Jaime, María del Carmen Escobar Villanueva, Mailinalli Brianza Padilla, Gonzalo Vázquez Palacios, dan Fransisco Javier Alarcón Aguilar. 2016. Chronic Unpredictable Mild stress Generates Oxidative Stress and Systemic Inflammation in Rats. Physiology & Behavior. 161 : 15-23. [Crossref]
• Mahoney, Megan M. 2010. Shift Work, Jet Lag, and Female Reproduction. International Journal of Endocrinology. pp 1 – 6. [Pubmed]
• Maramis, Margarita Maria. (2015). Mechanism of Impaired Spatial Working Memory Function and Cognitive Flexibility Through HSP70, IL-6,5-HT, BDNF and pCREB in the White Rat (Rattus norvegicus) Model of Depression. Doctoral’s Disertation, Universtas Airlangga 104p (in Bahasa). [Pubmed]
• Pasiak, T., Aswin, Susilowati R. 2005. Correlation of Dopamine D1 Receptors in the Prefrontal Cortex of Rats (Rattus novergicus) With Working Memory After Chronic Stress. Sains Kesehatan. 3 (18) : 383 – 397 (in Bahasa)
• Peng X, Chen Y. 2023. The Emerging Role Of Circadian Rhythms In The Development And Function Of Thermogenic Fat. Front Endocrinol (Lausanne). Pp : 1-10. [Pubmed]
• Puriastuti, Alifia Candra, Margarita M. Maramis, and Jimmy Januar Annas. (2017) Chronic Stress Reduces the Thickness of Rattus Norvegicus Antral Follicle Granulosa Cell Layers. Jurnal Biosains Pascasarjana Vol 19 No. 3
• Scheer, Frank A.J.L., Christopher J. Morris, Steven A. Shea. 2013. The Internal Circadian Clock Increases Hunger and Appetite In The Evening Independet of Food Intake and Other Behaviors. Obesity a Reasearch Journal. Vol 21 (3). 421-423. [Pubmed]
• Symonds, Jennifer LH. Emotional and Motivational Engagement at School Transition: A Qualitative Stage-Environment Fit Study. J Early Adolesc. 2016; 36 (1): 54–85 . [Crossref]
• Teruel, Mary. 2022. Circadian Clocks Play a Key Role in Fat Cell Growth. Weil Cornell Medicine News Room.
• Willy, Rahmat. (2016). The Effect of Frequency of Exposure to Light Stressors During Pregnancy on the Number and Apoptosis Index of Brain Neuron Cells in Newborn Mice (Mus musculus). Master’s Thesis, Universitas Airlangga 70p (in Bahasa).