THE ANTIOXIDANT ROLE OF STORAX IN BORON COMPOUNDS INDUCED HACAT CELLS

Year 2022, , 280 - 288, 25.12.2022

Buket Er Urgancı , Cihangir Doğan , Selda Şimşek , İbrahim Açıkbaş

https://doi.org/10.33457/ijhsrp.1121869

Abstract

Background: The skin is the first line of defense against microbial and chemical agents. Keratinocytes, represent the major component of the skin. Storax is thought to have antioxidant, anti-inflammatory and antimicrobial effects. some substances in storax have a cytotoxic effect and that storax can be a source of oxidative stress. Boron compounds have a wide physiological effect on biological systems at low concentrations, are toxic at high concentrations. The aim of this study is to evaluate the oxidative effect of storax on boron compounds treated HaCaT keratinocytes in vitro. Methods: In order to determine the effect of boron compounds on cell viability and 50% lethal dose, the MTT method was employed, and the IC50 dose was found to be 1000 μg/ml borax and 250 μg/ml colemanite at 24th hour. To determine the antioxidant activity of storax cells treated with borax and colemanite with or without storax and then the oxidative stress index, SOD, GPx and MDA levels were evaluated with ELISA.
Results: storax reduces the oxidative stress index through GPX, SOD and MDA activities.
Conclusion: When all the results are evaluated, the idea arises, that storax can be used as a possible therapeutic agent in skin.

Keywords

Borax, Colemanite, Keratinocyte, Skin, Storax

References

• [1] Proksch E, Brandner JM, Jensen JM. The skin: An indispensable barrier. Exp. Dermatol. 2008;17:1063–1072.
• [2] Luger TA, Schwarz T. Evidence for an epidermal cytokine network. Journal of Investigative Dermatology 1990;95(6); S100-S104.
• [3] Fujigawa H, Kondo S, Wang B, Shivji GM, Sauder DN. The expression and modulation of IFN-α and IFN-β in human keratinocytes. Journal of Interferon and Cytokine Research 1997;17;721–725.
• [4] Nimse, SB, Pal D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Adv. 2015;5;27986–28006.
• [5] Masaki, H. Role of antioxidants in the skin: Anti-aging effects. J. Dermatol. Sci. 2010;58;85–90.
• [6] Halliwell, B. Free radicals, antioxidants, and human disease curiosity, cause, or consequence? Lancet 1994;344:721-5.
• [7] Neeraj, P.J., Sing, S., Singh, J. Role of free radicals and antioxidants in human health and disease. Int J Curr Res Rev. 2013;5(19):14-22.
• [8] Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V . Method for the measurement of antioxidant activity in human fluids. Journal of clinical pathology 2001;54(5); 356-361.
• [9] Ayala A, Munoz MF, Arguelles S. Lipit peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014;360438:1-31.
• [10] Büyükuslu N, Yiğitbaşı T. Reaktif Oksijen Türleri ve Obezitede Oksidatif Stres. MÜSBED 2015;5(3):197-203
• [11] Lingbeck JM, O'Bryan CA, Martin EM, Adams JP, Crandall PG. Sweetgum: An ancient source of beneficial compounds with modern benefits. Pharmacogn Rev. 2015;9(17):1-11.
• [12] Ökmen G, Cantekin Z, Alam MI, Türkcan O, Ergün Y. Antibacterial and antioxidant activities of Liquidambar orientalis mill. various extracts against bacterial pathogens causing mastitis. Turkish Journal of Agriculture-Food Science and Technology 2017;5(8): 883-7.
• [13] Uluisik I, Karakaya HC, Koc A. The importance of boron in biological systems. J Trace Elem Med Biol 2018;45:156-162.
• [14] Yenmez, N. "The Importance of Boron Minerals in Turkey as a Strategic Mine." JOURNAL OF GEOGRAPHY-COGRAFYA DERGISI 2009;19: 59-94.
• [15] Uçkun Z. Esansiyel bir komponent: bor-borun günlük alımı ve fizyolojik etkileri. Türk Bilimsel Derlemeler Dergisi 2013;6(2):119-123.
• [16] Lewis RA, Hawley GG Hawley's condensed chemical dictionary. 2016 John Wiley & Sons,
• [17] Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radicalisation. Clin Biochem. 2004;37:277-85.
• [18] Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38:1103-11.
• [19] Yumru M, Savas HA, Kalenderoglu A, Bulut M, Celik H, et al. Oxidative imbalance in bipolar disorder subtypes: a comparative study. Prog Neuropsychopharmacol Biol Psychiatry 2009;33(6):1070-4.
• [20]Kosecik M, Erel O, Sevinc E, Selek S. Increased oxidative stress in children exposed to passive smoking. Int J Cardiol. 2005;100:61–4.
• [21] Harma M, Harma M, Erel O. Increased oxidative stress in patients with hydatidiform mole. Swiss Med Wkly 2003;133:563-536.
• [22] Gurbuz I, Yesilada E, Demirci B, Sezik E, Demirci F, et al. Characterization of volatiles and anti-ulcerogenic effect of Turkish sweetgum balsam (Styrax liquidus). J Ethnopharmacol. 2013;148(1):332–6.
• [23]Tian J, Shu Kang D, Pei Zhi Y. Optimization of porous SiO2 Antireflective (AR) Coatings Used in Encapsulating Solar Modules. Advanced Materials Research. 2013;739. Trans Tech Publications Ltd.
• [24]Sağdıç O, Özkan G, Özcan M, Özçelik, S. A Study on inhibitory effects of sığla tree (Liquidambar orientalis Mill. var. orientalis) storax against several bacteria. Phytotherapy Research 2005;19(6); 549-551.
• [25]Topal U, Sasaki M, Goto M, Otles S. Chemical compositions and antioxidant properties of essential oils from nine species of Turkish plants obtained by supercritical carbon dioxide extraction and steam distillation. Int J Food Sci Nutr. 2008;59(7-8):619-34.
• [26]Suzek H, Celik I, Dogan A, Yildirim S. Protective effect and antioxidant role of sweetgum (Liquidambar orientalis) oil against carbon tetrachloride-induced hepatotoxicity and oxidative stress in rats. Pharm Biol. 2016;54(3):451-7.
• [27] Aydıngöz M, Bulut S. Investigation of the Protective Effect of Sığla Oil Against Carbon Tetrachloride-Induced Toxication in Kidney. Journal of Applied Biological Sciences 2014;8(1):10–3.
• [28]Karadeniz B, Ulker Z, Alpsoy L. Genotoxic and cytotoxic effects of storax in vitro. Toxicol Ind Health. 2013;29(2):181-6.
• [29]Monzo-Beltran L, Vazquez-Tarragón A, Cerdà C., Garcia-Perez P., Iradi A., et al. One-year follow-up of clinical, metabolic and oxidative stress profile of morbid obese patients after laparoscopic sleeve gastrectomy. 8-oxo-dG as a clinical marker. Redox Biology 2017;12;389-402.
• [30]Kakarla P, Vadluri G, Reddy KS. Response of hepatic antioxidant system to exercise train-ing in aging female rat. J. Exp. Zoolog. A Comp. Exp.Biol. 2005;303;203-208
• [31] Türkez H, Geyikoğlu F, Tatar A, Keleş S, Ozkan A. Effects of some boron compounds on peripheral human blood. Z Naturforsch C J Biosci. 2007;62(11-12):889-96.