Aim: This study aimed to histological changes compare placental
villous basal lamina and amniotic membrane changes in complicated pregnancy.
Methods: Studies
were performed on the human placentas, delivered between 24-39 weeks of
gestation. Patients were separated equally into 4 groups (Control, preeclampsia (PE),
gestastional diabetes (GD), and HELLP syndrome groups)
Placental
tissue samples were dissected and fixed in 10% neutral formalin buffer. Routine
paraffin tissue protocol was followed. Some of sections were stained with
Periodic Acid Schiff. Remaining sections were stained with integrin alpha-6
antibody.To define expression percentage, mean of the staining area/total
staining area ratio were calculated. The statistical significance of the
expression percentages was compared by One Way ANOVA and Tukey tests with SPSS
Statistics V24 software.
Results: In PAS-stained preeclamptic, HELLP and gestational
diabetes groups placental villous basal lamina and vasculo-syncytial membranes were thicker than the
control group. A significant difference was observed in all 3 groups compared
to the control group the placental villous basal lamina
thickness of the HELLP group
was found to be significantly different from all three groups. In chorionic
villi of HELLP group, dense integrin expression was found in placental villous basal lamina similar to that in GD and preeclampsia groups. The HELLP group was
significantly different from all groups.
Conclusion: In preeclampsia, gestational diabetes and HELLP
placentas, placental viiöz basal lamina and amniotic membrane significantly thickened and
structural changes were observed.
Dicle University
13-TF-158
13-TF-158
Primary Language | English |
---|---|
Subjects | Evolutionary Biology (Other) |
Journal Section | Article |
Authors | |
Project Number | 13-TF-158 |
Publication Date | August 27, 2019 |
Submission Date | July 3, 2019 |
Acceptance Date | July 22, 2019 |
Published in Issue | Year 2019 |
https://upload.wikimedia.org/wikipedia/commons/2/20/DOAJ_logo.pnghttps://upload.wikimedia.org/wikipedia/commons/2/20/DOAJ_logo.pnghttps://upload.wikimedia.org/wikipedia/commons/2/20/DOAJ_logo.pnghttps://upload.wikimedia.org/wikipedia/commons/2/20/DOAJ_logo.png