Factor XIII (FXIII) Val34Leu is the most important
polymorphism of the A subunit in factor XIII gene. The aim of the present
study was to develop an efficient method based on real time PCR with
Fluorescence resonance energy transfer (FRET) detection and melting curve
analysis for the detection of Val34Leu polymorphism.
Specific primers were used to amplify the relevant fragment of the factor
XIII-A gene and fluorescence resonance energy transfer hybridization
probes were used for detection in a Rotor-Gene Q 5Plex HRM platform.
Melting temperature (Tm) for the wild type was at 68.8, while Tm for
homozygote mutant was at 63.8; heterozygote demonstrated both peaks.
Our results showed that primers and probes propoesd in this study
demonstrated a high specificity to identify wild type , heterozygous and
homozygous mutantgenotypes.
Due to the increasing molecular diagnosis in developing countries and the
importance of identifying polymorphisms, this real time PCR assay is of
great importance. An important advantage of this approach is the high
sensitivity and specificity.
Birincil Dil | İngilizce |
---|---|
Konular | Sağlık Kurumları Yönetimi |
Bölüm | Article |
Yazarlar | |
Yayımlanma Tarihi | 26 Ocak 2016 |
Gönderilme Tarihi | 13 Ekim 2015 |
Yayımlandığı Sayı | Yıl 2016 Cilt: 1 Sayı: 1 |
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