Alpha S1-Casein Gene Polymorphısm in Nigerian Balami Sheep Breed Indigenous to Mubi

Year 2019, Volume: 2 Issue: 3, 231 - 242, 28.12.2019

İsmaila Yada Sudi , Mohammed Shuaibu Malachi Albert Tizhe

https://doi.org/10.38001/ijlsb.631569

Cited By: 1

Abstract

The study was conducted on five Balami sheep breed to determine
and characterize the alpha S1 casein gene. Five blood samples were collected
from each animal intravenously using sterile needle and syringe. The blood
samples were placed in labelled tubes containing ethylene diamine tetraacetic
acid (EDTA). The blood samples were transported in on ice cold chain container
to the laboratory for analysis. Quick-DNA Miniprep ^TM kit used for
DNA extraction and amplified using forward and reverse primers (CSN1S1F 5’-ACCCCTCAGGTACCCTAAGAAA-3’
and CSN1S1R 5’-GTTTATCCCCCACACTGCATTC-3’). The gene was sequenced and blasted against the NCBI
database. Single nucleotide polymorphism analysis was performed to ascertain
the variations. Multiple sequence alignment and phylogenetic analysis within
and with the reference sequence was conducted online bioinformatics tools.
Result from the analysis reveals that, the extracted DNA were found on
chromosome 6, intron 16 and exon 17. The Balami breeds of sheep showed total
number of polymorphic and monomorphic site of 68 and 600 respectively, and
percentage of polymorphism of 10.18%. The Balami breed showed one amino acid
substitution and genetic variation within breeds. Complete molecular
characterization, genotyping and determination of allele frequency of alpha S1
gene in Balami breed of sheep indigenous to Nigeria and its variations is
recommended for further research. 

Keywords

Balami breeds, casein gene, genetic variation

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