Cytotoxic, apoptic and necrotics effects of Agrocybe cylindracea (DC.) Maire on breast cancer cells MCF-7 and L929

Abstract

The cytotoxicity, apoptotic and necrotic effects of Agrocybe cylindracea were investigated.The tissue fragments from taken dry basidiocarps were developed by tissue culture method in malt extract agar.Three pellets were taken from the secondary mycelium and its development completed in 7 days at 140 rpm at 27 °C in nutrient broth.In cytotoxicity studies, sample extract concentrations were applied to MCF-7 and L929 fibroblast cells as 5mg/ml, 2.5 mg/ml, 1.25 mg/ml,0.625 mg/ml and 0.3125 mg ml. The WST-1 solution was added and the absorbance was measured in a microplate reader at a wavelength of 440 nm as a result of the incubation. Apoptosis and necrosis were detected in the same cell line as the double staining experiment. Binary staining solution was prepared and MCF-7 and L929 Fibroblast cells were applied at the same concentrations.At the end of the incubation,measurements were done at 20X magnification in a fluorescence microscope with FITC and DAPI filter

Keywords

• Agrocybe cylindracea, Apoptotic effect, Cancer, Cytotoxicity, Necrotic effect

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