Antibacterial Effect of Methanolic Extract of Rosa damascena on Standard Bacteria Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa in Vitro

Abstract

Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa are the most important bacteria causing nosocomial infections that have multiple antibiotic resistance. These bacteria can also cause food poisoning and gastrointestinal diseases. Difficulties in treating infections caused by these bacteria have made it a reason to consider alternative medicines, including medicinal plants. In this descriptive-in vitro study, after collecting and drying under appropriate conditions, the extract was prepared by Soxhole method at concentrations of 20 mg/ml to 400 mg/ml. S. aureus, B. cereus, E. coli and P. aeruginosa were cultured separately in Mueller Hinton Agar medium. MIC and MBC of Rosa damascena methanolic extract were evaluated by Agar Well Diffusion and Dilution Test. The results showed that the antibacterial effects of methanolic extract of R. damascena against Gram-positive bacteria were more than Gram-negative bacteria, with the highest diameter of growth zone in S. aureus (26 mm) and B. cereus (24 mm) observed. MIC and MBC of methanolic extract of R. damascena on S. aureus, B. cereus and E. coli and P. aeruginosa were 6.25 -12.5, 12.5 -25, 50-100, 100-200 mg/ml, respectively. Considering the antimicrobial effect of methanolic extract of R. damascena on S. aureus, B. cereus and E. coli and P. aeruginosa in vitro, by evaluating its effects in vivo, it can be used as an alternative to conventional chemical drugs in the treatment of infections.

Keywords

• Antibacterial effects
• Extract
• Rosa damascena
• in vitro

References

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