Effect of Copper Oxide Nanoparticles on Prostate Cancer PC3 Cells

Abstract

Copper oxide (CuO NPs) nanoparticles were synthesized by green synthesis and characterized by UV-Vis spectroscopy, FT-IR spectroscopy, SEM and EDX analysis. The shape of the CuO nanoparticles obtained is approximately spherical and their particle sizes range from 40 to 85 nm. According to the experimental results, two peaks at 305 and 318 nm in the 270-400 nm range in the UV-Vis absorption spectrum and one peak at 536 cm-1 in the FT-IR spectrum confirm the presence of copper oxide nanoparticles. In addition, the presence of biomolecules in aesculus hippocastanum(horse chestnut) extract on the surface of the copper oxide nanoparticles synthesized by green synthesis was determined by the peaks seen in the range of 3300 cm-1 - 1000 cm-1 in the FTIR spectrum. The images, shape and size of the copper oxide nanoparticles were determined by SEM, and the weight percent of the elements were determined by EDX. PC3 prostate cell lines were used in the study. After measuring the cytotoxic effect of the agents used in the study on prostate cancer cells, the apoptotic effect of this effect was determined by Hoechst/propidium iodide (HOPI) staining. Graphpad prism program was used to compare all parameters between groups, and "one-way analysis of variance" (one-way ANOVA) method and dunnet's test were applied to test whether there was any difference between at least two groups. It has been determined that the drug obtained from copper oxide nanoparticles has a cytotoxic effect on PC3 prostate cancer cells and this effect occurs through the apoptosis pathway.

Keywords

• Green synthesis
• Copper oxide nanoparticle
• Prostate cancer
• PC3 prostate cell

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