Plastik Kompozit Destek Üzerine İki Endüstriyel Şus ile Oluşturulan Biyofilm Yapılarının Tespit Edilmesi

Abstract

Bu çalışmada Candida intermedia NRRL Y-8278 ve Kluyveromces marxianus NRRL Y-8281 endüstriyel mayalarının farklı PCS destekler üzerindeki birim alandaki koloni sayıları (CFU) geliştirilip, hesaplanmıştır. Farklı destekler üzerindeki analiz yöntemi olarak Stripping-Sand metodu kullanılmıştır. Her bir destek için (PCS₁, PCS₂, PCS₃, PCS₄) hesaplamalar yapılmıştır. Kullanılan kültürlerin, plastik destekler üzerinde biyofilm yapıları meydana getirdiği tespit edilmiştir. K. marxianus’un PCS₂ desteği üzerinde 2.9×10⁸ CFU g^-1 ve 1.6×10¹⁰ CFU mL^-1 değerlerinde koloni oluşturduğu ve  C. intermedia’dan daha iyi biofilm performansı gösterdiği gözlemlendi. Kullanılan plastik desteklerin içerdiği polipropilen gıda, biyomedikal, hastane ve sağlık laboratuvarları gibi birçok yerde bazı cihazların yapısında bulunmaktadır. Ayrıca çalışmamızın sonuçları, polipropilen gibi bazı desteklerin ve endüstriyel mayaların kullanıldığı gıda ile biomedikal teknolojileri ve endüstriyel birçok alanda yararlı olacağı aynı zamanda bu materyallerin önem arz ettiği kanısına varılmıştır.

 

Keywords

• Biyofilm,biyopolimer,CFU,PCS

The Determining of the Biofilm Form by Two Industrial Strains on Plastic Composite Supports

Abstract

In this paper, the biofilm formation of industrial yeasts Candida intermedia NRRL Y-8278 and Kluyveromces marxianus NRRL Y-8281 yeast were grown on various plastic composite supports (PCS) to analyze colony forming unit (CFU). The biofilm was performed using Stripping-Sand method for yeasts on the PCS (PCS₁, PCS₂, PCS₃, PCS₄). The biofilm formation on the yeasts was observed on the PCS. K. marxianus and C. intermedia took place the apparent the biofilm population CFU of each yeast on plastic supports. The best biofilm population was performed 2.9×10⁸ CFU g^-1 by K. marxianus on PCS₂ support than C. intermedia. The calculation of biofilm in   CFU mL^-1 on each yeast also was analyzed 1.6×10¹⁰ by K. marxianus on the PCS₂.  K. marxianus had better the biofilm values on the PCS than C. intermedia. The results of this work will be useful on the some areas that this supports (polypropylene) used in such as food, biomedical and industrial companies and to realize about structure of yeasts and polypropylene supports in the literatures.

Keywords

• Biofilm,biopolymer,CFU,PCS

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