Comparison of the Aflatoxin levels in Fish Feeds from Rainbow Trout Farms Localized at Different Regions (Adana-Ağrı, TURKEY)

Abstract

Aflatoxins levels in different fish feeds obtained from two different rainbow trout (Oncorhynchus mykiss) farms located in Adana and Ağrı regions were determined between May and July 2017. During the experiment period, ''a drought status'' was reported according to the Standardized Precipitation Index of Turkey by Turkish State Meteorological Service. According to precipitation index records, while Adana was reported as slightly humid, Ağrı was slightly dry in the period between May and July 2017. The aflatoxin analyses of fish feed samples was carried out in the laboratories of the Ministry of Food, Agriculture and Livestock in Adana, Turkey. The aflatoxin levels of the samples were resolved by liquid-solid extraction, immune-affinity pillar clean-up using a HPLC-FD (High Performance Liquid Chromatography with Fluorescence Detection). The detection limits of aflatoxin were 0.50, 0.23, 0.73, 0.20 ppb for AFB1, AFB2, AFG1, AFG2, respectively. The results showed that, aflatoxin levels of fish feeds were below the detection limits in all samples.Aflatoxins levels in different fish feeds obtained from two different rainbow trout (Oncorhynchus mykiss) farms located in Adana and Ağrı regions were determined between May and July 2017. During the experiment period, ''a drought status'' was reported according to the Standardized Precipitation Index of Turkey by Turkish State Meteorological Service. According to precipitation index records, while Adana was reported as slightly humid, Ağrı was slightly dry in the period between May and July 2017. The aflatoxin analyses of fish feed samples was carried out in the laboratories of the Ministry of Food, Agriculture and Livestock in Adana, Turkey. The aflatoxin levels of the samples were resolved by liquid-solid extraction, immune-affinity pillar clean-up using a HPLC-FD (High Performance Liquid Chromatography with Fluorescence Detection). The detection limits of aflatoxin were 0.50, 0.23, 0.73, 0.20 ppb for AFB1, AFB2, AFG1, AFG2, respectively. The results showed that, aflatoxin levels of fish feeds were below the detection limits in all samples. 

 

Keywords

• Aflatoxin,Fish feed,HPLC analysis,Rainbow trout farm

Farklı Bölgelerde (Adana-Ağrı, TÜRKİYE) Bulunan Gökkuşağı Alabalığı Çiftliklerindeki Balık Yemlerinde Aflatoksin Düzeylerinin Karşılaştırılması

Abstract

Mayıs ve Temmuz 2017 tarihleri arasında Adana ve Ağrı bölgelerinde bulunan iki farklı gökkuşağı alabalığı (Oncorhynchus mykiss) çiftliğinden elde edilen farklı balık yemlerinde aflatoksin seviyeleri belirlendi. Çalışmamıza ait dönemde Türkiye aylık standart yağış indeksine göre kuraklık durumu kaydedilmiştir. Kayıtlarımıza göre Ağrı ili Mayıs-Temmuz aylarında hafif kurak, Adana ili ise hafif nemli bir hava göstermiştir. Yem örneklerinin analizi T.C. Adana Gıda Tarım ve Hayvancılık Laboratuvarlarında yürütülmüştür. Numuneler floresan saptama (HPLC-FD) ile yüksek performanslı sıvı kromatografisi kullanılarak, sıvı-katı özütleme immune-affinity column clean-up metodu ile belirlenmiştir. Aflotoksin tespit limiti AFB1, AFB2, AFG1, AFG2 için sırasıyla 0,50; 0,23; 0,73; 0,20 ppb, olarak belirlenmiştir. Örneklenen tüm balık yemlerinde aflatoksin düzeylerin tespit edilebilir değerin altında olduğu bulunmuştur.

Keywords

• Aflatoksin,balık yemi,HPLC analizi,gökkuşağı alabalığı çiftliği

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