Bacillus subtilis Bakterisi ile Metribuzin Herbisitinin Biyoıslahının Yapay Tarla Düzeneğinde Araştırılması

Abstract

Türkiye’de domates yetiştiriciliğinde seçkin bir herbisit olan Metribuzinin (C8H14N4OS) biyoıslahı Bacillus subtilis bakterisiyle çalkalanmalı kültür koşullarında ve yapay tarla düzeneğine araştırılmıştır. Bakteri Türkiye Elazığ ilinden bir tarım arazisinden toplanan topraktan izole edilmiştir. Bölge daha önce metribuzine maruz kalmayan bir bölgedir. 1750 mg L-1 konsantrasyonda (çiftçler için tavsiye edilen konsantrasyon)  100’er mL’lik beş adet aparat hazırlanmıştır ve metribuzin her bir erlenmayer şişesine ilave edilmiştir. Her bir zenginleştirilen solüsyon × 10⁷ Koloni oluşturan birey (KOB) içermektedir. Bu şişeler 160 rpm de 28°C’de steril koşullarda 17 gün boyunca çalkalanmıştır. 24 saatlik dilimlerde, her bir örnek toplanmıştır ve DR/890 kolorimetre ile kimyasal oksijen ihtiyacı (KOİ) kapalı refluks yöntemi ışığında belirlenirken Biyokimyasal Oksijen İhtiyacı (BOİ₅) standart method 5210D ye göre oksitoplarla belirlenmiştir. Çalışma sonuçlarına göre en iyi giderim performansı 100 mL Bacillus subtilis türünde KOİ ve BOİ₅ bazında 17 günde %98 oranında görülmüştür. Yapay tarla çalışmasının sonuçlarına göre KOİ ve BOİ₅ parametrelerinde 2. haftanın sonunda sırasıyla %99.4 ve %96 olarak görülmüştür. Bu deneyler göstermiştir ki Bacillus subtilis alıcı ortamlardaki metribuzin gieriminde seçkin bir türdür.

Keywords

• Bakteri,biyoıslah,herbisit,metribuzin

Investigation of the Bioremediation of Herbicide Metribuzin with Bacillus subtilis Bacteria at Artifical Agricultural Field

Abstract

The bioremoval of metribuzin (C₈H₁₄N₄OS) which is one of the selective herbicide in tomato farming in Turkey was investigated with Bacillus subtilis bacteria under agitated culture conditions and artifical agricultural field. The bacterium was isolated in collected soil samples from one of the agricultural area in Elazig province of Turkey. The area is unexposed to metribuzin before. Five apparatuses were set up and 100 mL of metribuzin in 1750 ppm concentration (advised concentration of tomato farmers) was added to each Erlenmayer flasks. 5, 10, 20, 50 and 100 mL of enriched Bacillus subtilis solutions (in sabouraud dextrose broth) added to these flasks. Each mL of the enriched solutions contains ×10⁷ Colony forming unit (CFU). These flasks were shaken at 160 rpm at 28 ⁰C in sterile conditions for 17 days. In 24 hours intervals, each sample was collected to flasks and chemical oxygen demand (COD) was determined with DR/890 colorimeter by the line of closed reflux method while Biochemical oxygen demand (BOD₅)experiments were performed via standard methods 5210D with oxitops. As a result of the study, best removal performance observed in 100 mL Bacillus subtilis as 98% at 17 days in COD. In a soil study, 100 mL mixture of Bacillus subtilis consortia used for bioremediation studies. According to the results of artifical agricultural field study, the bioremediation seen at COD and BOD₅ parameters are 99.4% and 96% respectively at the end of 2. week. This experiments have focused that, Bacillus subtilis should be a selective type for bioremediation of metribuzin on receiving environments.

Keywords

• Bacteria,bioremoval,herbicide,metribuzin

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