Pharmacological potential of fungal endophytes associated with the genus Ocimum L.

Abstract

Endophytes are a rich source of secondary metabolites such as tannins, phenolics, and alkaloids. Endophytic fungi have potential as antioxidants, antimicrobials, anticancer agents, antidiabetic agents, hepatoprotectants, growth promoters, and immunomodulators. Recent studies have shown that endophytes are a valuable source of undiscovered biomolecules. As a result, endophytic isolates from medicinal plants can be used in the pharmaceutical, industrial, and agricultural sectors. Ocimum species, for example, have several medicinal properties and are used in traditional medicine. Fungal endophytes have a strong association with Ocimum plants. Previous research has shown that the fungal endophytes of Ocimum sanctum produce phytochemicals such as alkaloids, terpenoids, cardiac glycosides, flavonoids, terpenes, and volatile compounds. Additionally, fungal endophytes have a direct impact on the medicinal value of the genus Ocimum. This review aimed to discuss the pharmacological properties and diversity of endophytic fungi associated with the genus Ocimum.

Keywords

• Endophytes
• Medicinal properties
• Novel biomolecules
• Ocimum plants
• Pharmacology

References

1. Abdel-Rahman, W., Abdel-Motaal, F., El-Zayat, S., & El-Sayed, M. (2019). Phytochemical screening, Antioxidative and anti-α-amylase properties of endophytic fungal extracts isolated from Ocimum basilicum. Journal of Biological Studies, 2(2), 31–41.
2. Atiphasaworn, P., Monggoot, S., Gentekaki, E., Brooks, S., & Pripdeevech, P. (2017). Antibacterial and antioxidant constituents of extracts of endophytic fungi isolated from Ocimum basilicum var. thyrsiflora leaves. Current Microbiology, 74(10), 1185–1193.
3. Bagur, H., Poojari, C.C., Melappa, G., Rangappa, R., Chandrasekhar, N., & Somu, P. (2020). Biogenically synthesized silver nanoparticles using endophyte fungal extract of Ocimum tenuiflorum and evaluation of biomedical properties. Journal of Cluster Science, 31(6), 1241–1255. https://doi.org/10.1007/s10876-019-01731-4
4. Bodhankar, M. G. (2014). Antimicrobial activity of endophytic fungi isolated from Ocimum sanctum Linn. American International Journal of Contemporary Scientific Research, 1(3), 17–22.
5. Chaeprasert, S., Piapukiew, J., Whalley, A. J., & Sihanonth, P. (2010). Endophytic fungi from mangrove plant species of Thailand: Their antimicrobial and anticancer potentials. Botanica Marina, 53(6), 555–564.
6. Chowdhary, K., & Kaushik, N. (2015). Fungal Endophyte Diversity and Bioactivity in the Indian Medicinal Plant Ocimum sanctum Linn. PLOS ONE, 10(11), e0141444. https://doi.org/10.1371/journal.pone.0141444
7. Dhama, K., Sharun, K., Gugjoo, M.B., Tiwari, R., Alagawany, M., Iqbal Yatoo, M., Thakur, P., Iqbal, H. M., Chaicumpa, W., & Michalak, I. (2021). A comprehensive review on chemical profile and pharmacological activities of Ocimum basilicum. Food Reviews International, 1–29.
8. Gangadevi, V., & Muthumary, J. (2007). Preliminary studies on cytotoxic effect of fungal Taxol on cancer cell lines. African Journal of Biotechnology, 6(12), 1382-1386.

9. Haque, M.A., Hossain, M.S., Rahman, M.Z., Rahman, M.R., Hossain, M.S., Mosihuzzaman, M., Nahar, N., & Khan, S. I. (2005). Isolation of bioactive secondary metabolites from the endophytic fungus of Ocimum basilicum. Journal of Pharmaceutical Sciences, 4(2), 127–130.
10. Jalil, M.T.M., & Ibrahim, D. (2021). Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 inhibits the growth of skin pathogenic bacterium and attenuates biofilms of methicillin-resistant Staphylococcus aureus. The Malaysian Journal of Medical Sciences, 28(4), 24.
11. Kumar A, S., Reddy G.B., & Kulal, A. (2014). In-vitro studies on α-amylase, α-glucosidase and aldose reductase inhibitors found in endophytic fungi isolated from Ocimum sanctum. Current Enzyme Inhibition, 10(2), 129-136.
12. Lai, D., Brötz-Oesterhelt, H., Müller, W.E.G., Wray, V., & Proksch, P. (2013). Bioactive polyketides and alkaloids from Penicillium citrinum, a fungal endophyte isolated from Ocimum tenuiflorum. Fitoterapia, 91, 100–106. https://doi.org/10.1016/j.fitote.2013.08.017
13. Madagundi, S., Habbu, P., Jagadish, K.S., Shukla, S., Salagare, M., & Kulkarni, V.H. (2013). Free radical scavenging and in vitro immunomodulatory activities of endophytic fungi of Ocimum sanctum L. Farmacia, 61, 330–343.
14. Mohammad, S., Khandaker, M.S.U., & Nilufar, N. (2015). Oxygenated secondary metabolites from endophytic fungi isolated from Ocimum sanctum. Journal of Basic & Applied Sciences, 11, 434-439.
15. Omar, N.A. (2015). Isolation of endophytic fungal from Thai Basil Leaves (Ocimum basilicum var. thyrsiflora) and determination of their antimicrobial activity [Doctoral dissertation, Faculty of Agro-Based Industry].
16. Ismail, N. (2015). Isolation and screening antimicrobial activity of endophytic fungal from african blue basil (Ocimum kilimandscaricum) leaves [Doctoral dissertation, Faculty of Agro-Based Industry].
17. Nahak, G., Mishra, R.C., & Sahu, R.K. (2011). Taxonomic distribution, medicinal properties and drug development potentiality of Ocimum (Tulsi). Drug Invention Today, 3(6), 95-113.
18. Nur Amalina, S. (2015). Isolation and screening of antimicrobial activity of endophytic fungal isolates from lemon Thai Basil (Ocimum citriodorum Vis.) leaves [Doctoral dissertation, Faculty of Agro Based Industry].
19. Pattanayak, P., Behera, P., Das, D., & Panda, S.K. (2010). Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview. Pharmacognosy Reviews, 4(7), 95–105. https://doi.org/10.4103/0973-7847.65323
20. Sabra, M., Aboulnasr, A., Franken, P., Perreca, E., Wright, L.P., & Camehl, I. (2018). Beneficial root endophytic fungi increase growth and quality parameters of Sweet Basil in heavy metal contaminated soil. Frontiers in Plant Science, 9. https://doi.org/10.3389/fpls.2018.01726
21. Schulz, B., & Boyle, C. (2005). The endophytic continuum. Mycological Research, 109(6), 661–686.
22. Shebany, Y.M., Abdel-Kader, A.S., & Ebeid, A.F.A. (2013). Influence of endophytic fungi on growth and essential oil composition of Ocimum basilicum L. Egyptian Journal of Botany, 53(1), 133–148.
23. Shekhawat, S., & Shah, G. (2013). Isolation, characterization and determination of antibacterial activity of bacterial and fungal endophytes from Ocimum sanctum and phytochemical analysis. International Journal of Pharmacy and Biological Sciences, 4 (4), 600-607.
24. Shoeb, M., Hoque, M.E., Thoo-Lin, P.K., & Nahar, N. (2013). Anti-pancreatic cancer potential of secalonic acid derivatives from endophytic fungi isolated from Ocimum basilicum. Dhaka University Journal of Pharmaceutical Sciences, 12 (2), 91–95.
25. Shukla, S.T., Kulkarni, V.H., Habbu, P.V., Jagadeesh, K.S., Patil, B.S., & Smita, D.M. (2012). Hepatoprotective and antioxidant activities of crude fractions of endophytic fungi of Ocimum sanctum Linn. in rats. Oriental Pharmacy and Experimental Medicine, 12(2), 81–91. https://doi.org/10.1007/s13596-012-0061-7
26. Stone, J.K., Polishook, J.D., & White, J.F. (2004). Endophytic fungi. Biodiversity of Fungi. Elsevier Academic Press, 241–270.
27. Sudha, V., Govindaraj, R., Baskar, K., Al-Dhabi, N.A., & Duraipandiyan, V. (2016). Biological properties of endophytic fungi. Brazilian Archives of Biology and Technology, 59, 1-7.
28. Taufiq, M.M.J., & Darah, I. (2018). Fungal endophytes isolated from the leaves of a medicinal plant, Ocimum sanctum L. and evaluation of their antimicrobial activities. African Journal of Microbiology Research, 12(26), 616–622. https://doi.org/10.5897/AJMR2018.8812
29. Taufiq, M.M.J., & Darah, I. (2019). Antibacterial activity of an endophytic fungus Lasiodiplodia pseudotheobromae IBRL OS-64 residing in leaves of a medicinal herb, Ocimum sanctum Linn. Journal of Applied Biology and Biotechnology, 7(2), 3–1.
30. Yadav, R., Singh, A.V., Joshi, S., & Kumar, M. (2015). Antifungal and enzyme activity of endophytic fungi isolated from Ocimum sanctum and Aloe vera. African Journal of Microbiology Research, 9(29), 1783–1788.
31. Zaidi, K.U., Mani, A., Ali, A.S., & Ali, S.A. (2013). Evaluation of Tyrosinase producing endophytic fungi from Calotropis gigantea, Azadirachta indica, Ocimum tenuiflorum and Lantana camara. Annual Research & Review in Biology, 3(4), 389-396.
32. Zhang, H.W., Song, Y.C., & Tan, R.X. (2006). Biology and chemistry of endophytes. Natural Product Reports, 23 (5), 753-771.