Medical physiological perspective to biochemical assays and GC-MS results of corn tassel

Abstract

Corn tassel (Zea may L.) is rich in phenolic compounds including flavonoids and anthocyanins. The aim of this study is to consider the results of the contents of phenolics (TPC), flavonoids (TFC), flavonol (TF), anthocyanins (TAC), alfa-amylase inhibitory activity, and antioxidant activity including FRAP and metal chelating capabilities (MCC) as potential Antiviral and anti-Rheumatoid arthritis. Significantly high levels of antioxidant capacity, total flavonol and alfa-amylase inhibition were found in ethanolic extracts of corn tassels. It was found that their concentrations are TPC= 40 mg GA/g, TFC= 13 mg QE/g, TF= 45 mg R/g, and TAC= 8 mg cyanidin-3-glucoside/g based on dry extract. Additionally, the extracts showed relatively higher antioxidant activities due to metal chelating capabilities (MCC) were found to be 217 mg Fe2+/g dry extract. From the GC-MS analysis, corn tassel was found to be good source of arctigenin that has antiviral and anti- rheumatic properties. Further, the extracts of corn tassels showed significantly higher α-amylase inhibitory activity up to 90 %. Thus, it was concluded that extracts of corn tassels may be considered as pharmacological potential in rheumatoid and antiviral treatment.

Keywords

• Corn tassel;
• Bioactive compounds
• Antioxidant activity

References

1. Abraham, A.M., Quintero, C., Carrillo-Hormaza, L., Osorio, E., & Keck, C.M. (2021). Production and Characterization of Sumac PlantCrystals: Influence of High-Pressure Homogenization on Antioxidant Activity of Sumac (Rhus coriaria L.). Plants (Basel, Switzerland), 10(6). https://doi.org/10.3390/PLANTS10061051
2. Ademiluyi, A.O., & Oboh, G. (2013). Aqueous Extracts of Roselle (Hibiscus sabdariffa Linn.) Varieties Inhibit α-Amylase and α-Glucosidase Activities In Vitro. Journal of Medicinal Food, 16(1), 88–93. https://doi.org/10.1089/JMF.2012.0004
3. Aziz, M., Saeed, F., Ahmad, N., Ahmad, A., Afzaal, M., Hussain, S., Mohamed, A.A., Alamri, M.S., & Anjum, F.M. (2021). Biochemical profile of milk thistle (Silybum Marianum L.) with special reference to silymarin content. Food Science & Nutrition, 9(1), 244–250. https://doi.org/10.1002/FSN3.1990
4. Chakraborty, D., Gupta, K., & Biswas, S. (2021). A mechanistic insight of phytoestrogens used for Rheumatoid arthritis: An evidence-based review. Biomedicine and Pharmacotherapy, 133. https://doi.org/10.1016/J.BIOPHA.2020.111039
5. Do, Q.D., Angkawijaya, A.E., Tran-Nguyen, P.L., Huynh, L.H., Soetaredjo, F.E., Ismadji, S., & Ju, Y.H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3), 296–302. https://doi.org/10.1016/J.JFDA.2013.11.001
6. Dorman, H.J.D., Peltoketo, A., Hiltunen, R., & Tikkanen, M.J. (2003). Characterisation of the antioxidant properties of de-odourised aqueous extracts from selected Lamiaceae herbs. Food Chemistry, 83(2), 255–262. https://doi.org/10.1016/S0308-8146(03)00088-8
7. Duangpapeng, P., Ketthaisong, D., Lomthaisong, K., Lertrat, K., Scott, M.P., & Suriharn, B. (2018). Corn Tassel: A new source of phytochemicals and antioxidant potential for value-added product development in the agro-industry. Agronomy, 8(11), 242. https://doi.org/10.3390/agronomy8110242
8. Gao, Q., Yang, M., & Zuo, Z. (2018). Overview of the anti-inflammatory effects, pharmacokinetic properties and clinical efficacies of arctigenin and arctiin from Arctium lappa L. Acta Pharmacologica Sinica, 39(5), 787–801. https://doi.org/10.1038/APS.2018.32

9. Hosseinian, F.S., Li, W., & Beta, T. (2008). Measurement of anthocyanins and other phytochemicals in purple wheat. Food Chemistry, 109(4), 916 924. https://doi.org/10.1016/J.FOODCHEM.2007.12.083
10. Iqbal, E., Salim, K.A., & Lim, L.B.L. (2015). Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus (Airy Shaw) from Brunei Darussalam. Journal of King Saud University - Science, 27(3), 224–232. https://doi.org/10.1016/J.JKSUS.2015.02.003
11. Işil Berker, K., Güçlü, K., Tor, I., Demirata, B., & Apak, R. (2010). Total antioxidant capacity assay using optimized ferricyanide/Prussian blue method. Food Analytical Methods, 3(3), 154–168. https://doi.org/10.1007/S12161-009-9117-9/TABLES/7
12. Kalita, P., Tapan, B.K., Pal, T.K., & Kalita, R. (2013). Estımatıon of total flavonoıds content (TFC) and antı oxıdant actıvıtıes of methanolıc whole plant extract of bıophytum sensıtıvum lınn. Journal of Drug Delivery and Therapeutics, 3(4), 33 37. https://doi.org/10.22270/jddt.v3i4.546
13. Kessler, M., Ubeaud, G., & Jung, L. (2003). Anti-and pro-oxidant activity of rutin and quercetin derivatives. Journal of Pharmacy and Pharmacology, 55(1), 131–142.
14. Lee, J., Durst, R.W., Wrolstad, R.E., Barnes, K.W., Eisele, ; T, Giusti, ; MM, Haché, ; J, Hofsommer, ; H, Koswig, ; S, Krueger, D. A., Kupina, ; S, Martin, ; S.K., Martinsen, ; B.K., Miller, T. C., Paquette, ; F, Ryabkova, ; A, Skrede, ; G, Trenn, ; U, & Wightman, J.D. (2005). Determination of Total Monomeric Anthocyanin Pigment Content of Fruit Juices, Beverages, Natural Colorants, and Wines by the pH Differential Method: Collaborative Study. Journal of AOAC International, 88(5), 1269 1278. https://doi.org/10.1093/JAOAC/88.5.1269
15. Mihailović, V., Kreft, S., Benković, E.T., Ivanović, N., & Stanković, M.S. (2016). Chemical profile, antioxidant activity and stability in stimulated gastrointestinal tract model system of three Verbascum species. Industrial Crops and Products, 89, 141–151. https://doi.org/10.1016/J.INDCROP.2016.04.075
16. Mohsen, S.M., & Ammar, A.S.M. (2009). Total phenolic contents and antioxidant activity of corn tassel extracts. Food Chemistry, 112(3), 595 598. https://doi.org/10.1016/J.FOODCHEM.2008.06.014
17. Nickavar, B., & Yousefian, N. (2011). Evaluation of α-amylase inhibitory activities of selected antidiabetic medicinal plants. Journal Fur Verbraucherschutz Und Lebensmittelsicherheit, 6(2), 191–195. https://doi.org/10.1007/S00003-010-0627-6
18. Rajanandh, M.G., & Kavitha, J. (2010). Quantitative estimation of β-sitosterol, total phenolic and flavonoid compounds in the leaves of Moringa oleifera. International Journal of PharmTech Research, 2(2), 1409–1414.
19. Rice-evans, C.A., Miller, N.J., Bolwell, P.G., Bramley, P.M., & Pridham, J.B. (1995). The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radical Research, 22(4), 375–383. https://doi.org/10.3109/10715769509145649
20. Shabab, S., Gholamnezhad, Z., & Mahmoudabady, M. (2021). Protective effects of medicinal plant against diabetes induced cardiac disorder: A review. Journal of Ethnopharmacology, 265, 113328. https://doi.org/10.1016/j.jep.2020.113328
21. Shabgah, A.G., Suksatan, W., Achmad, M.H., Bokov, D.O., Abdelbasset, W.K., Ezzatifar, F., Hemmati, S., Mohammadi, H., Soleimani, D., Jadidi-Niaragh, F., Ahmadi, M., & Navashenaq, J.G. (2021). Arctigenin, an anti-tumor agent; a cutting-edge topic and up-to-the-minute approach in cancer treatment. European Journal of Pharmacology, 909. https://doi.org/10.1016/J.EJPHAR.2021.174419
22. Srivastava, D., & K, S. (2017). Arctigenin, A Plant Lignan With Tremendous Potential: A Review. International Journal of Current Research, 9(05), 50232–50237.
23. Sudan, R., Bhagat, M., Gupta, S., Singh, J., & Koul, A. (2014). Iron (FeII) chelation, ferric reducing antioxidant power, and immune modulating potential of Arisaema jacquemontii (Himalayan Cobra Lily). BioMed Research International, 2014. https://doi.org/10.1155/2014/179865
24. Taha, M.N., Krawinkel, M.B., & Morlock, G.E. (2015). High-performance thin-layer chromatography linked with (bio)assays and mass spectrometry - A suited method for discovery and quantification of bioactive components? Exemplarily shown for turmeric and milk thistle extracts. Journal of Chromatography A, 1394, 137 147. https://doi.org/10.1016/J.CHROMA.2015.03.029
25. Uddin, N., Hasan, M.R., Hossain, M.M., Sarker, A., Nazmul Hasan, A.H.M., Mahmudul Islam, A.F.M., Chowdhury, M.M.H., & Rana, M.S. (2014). In vitro α-amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit. Asian Pacific Journal of Tropical Biomedicine, 4(6), 473. https://doi.org/10.12980/APJTB.4.2014C1173
26. Wang, L.C., Yu, Y.Q., Fang, M., Zhan, C.G., Pan, H.Y., Wu, Y.N., & Gong, Z.Y. (2014). Antioxidant and antigenotoxic activity of bioactive extracts from corn tassel. Journal of Huazhong University of Science and Technology - Medical Science, 34(1), 131–136. https://doi.org/10.1007/s11596-014-1244-x
27. Xu, X., Piao, H.N., Aosai, F., Zeng, X.Y., Cheng, J.H., Cui, Y.X., Li, J., Ma, J., Piao, H.R., Jin, X., & Piao, L.X. (2020). Arctigenin protects against depression by inhibiting microglial activation and neuroinflammation via HMGB1/TLR4/NF-κB and TNF-α/TNFR1/NF-κB pathways. British Journal of Pharmacology, 177(22), 5224 5245. https://doi.org/10.1111/BPH.15261
28. Yefrida, Suyani, H., Alif, A., Efdi, M., & Aziz, H. (2018). Modification of phenanthroline method to determine antioxidant content in tropical fruits methanolic extract. Research Journal of Chemistry and Environment, 22(4), 28–35.
29. Zhong, Y., Lee, K., Deng, Y., Ma, Y., Chen, Y., Li, X., Wei, C., Yang, S., Wang, T., Wong, N.J., Muwonge, A.N., Azeloglu, E.U., Zhang, W., Das, B., He, J.C., & Liu, R. (2019). Arctigenin attenuates diabetic kidney disease through the activation of PP2A in podocytes. Nature Communications, 10(1). https://doi.org/10.1038/S41467-019-12433-W