Research Article

Phytochemical screening and in vitro biological activity of Amaranthus viridis growing in Northern Cyprus

Volume: 11 Number: 3 August 29, 2024
TR EN

Phytochemical screening and in vitro biological activity of Amaranthus viridis growing in Northern Cyprus

Abstract

In this study, phenolic and non-phenolic metabolites in different parts of A. viridis (from the Turkish Republic of Northern Cyprus) were characterized with reversed-phase high-performance liquid chromatography (RP-HPLC) with diode array detector and high-performance liquid chromatography (HPLC), respectively. In total, approximately twenty-five phenolic compounds including quercetin, chrysin, t-Ferulic acid, and sinapic acid as the most abundant secondary metabolites were identified. On the other hand, four organic acids as non-phenolic compounds quantitatively predominant were identified for the first time in A. viridis extracts. The seed and flower extract showed strong ferric-reducing capacity, radical scavenging activity for DPPH˙, phosphomolybdenum assay, metal chelating, and α-amylase inhibition activity by in vitro assays. Our results suggest that A. viridis widely used in the human diet in Cyprus is a source of numerous metabolites showing antioxidant and antibacterial potential.

Keywords

References

  1. Abdel-alim, M.E., Serag, M.S., Moussa, H.R., Elgendy, M.A., Mohesien, M.T., & Salim, N.S. (2023). Phytochemical screening and antioxidant potential of Lotus corniculatus and Amaranthus viridis. Egyptian Journal of Botany, 63(2), 1 17. http://dx.doi.org/10.21608/ejbo.2023.158720.2118
  2. Ahmed, S.A., Hanif, S., & Iftkhar, T. (2013). Phytochemical profiling with antioxidant and antimicrobial screening of Amaranthus viridis L. leaf and seed extracts. Open Journal of Medical Microbiology, 3, 164-171. http://dx.doi.org/10.4236/ojmm.2013.33025
  3. Akbar, M., Sherazi, I.N., Iqbal, M.S., Khalil, T., & Waqas, H.M. (2018). Antibacterial and antioxidant activities of Slender Amaranth Weed. Planta Daninha, 38, e020192974, 1-8. http://dx.doi.org/10.1590/S0100-83582020380100006
  4. Başyiğit, B., Sağlam, H., Köroğlu, K., & Karaaslan, M. (2020). Compositional analysis, biological activity, and food protecting ability of ethanolic extract of Quercus infectoria gall. Journal of Food Processing and Preservation, 44, e14692. https://doi.org/10.1111/jfpp.14692
  5. Blois, M.S. (1958). Antioxidant determinations by the use of stable free radical. Nature, 1199–1200.
  6. Chen, C.C., Huang, M.Y., Lin, K.H., & Hsueh, M.T. (2022). The effects of nitrogen application on the growth, photosynthesis, and antioxidant activity of Amaranthus viridis. Photosynthetica, 60(3), 420-429. http://dx.doi.org/10.32615/ps.2022.034
  7. CLSI, (2012). Performance Standards for Antimicrobial Disk Susceptibility Tests, Approved Standard (7th ed.). CLSI document M02-A11, Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA.
  8. Datta, S., Sinha, B.K., Bhattacharjee, S., & Seal, T. (2019). Nutritional composition, mineral content, antioxidant activity and quantitative estimation of water soluble vitamins and phenolics by RP-HPLC in some lesser used wild edible plants. Heliyon, 5, e01431, 1-37. https://doi.org/10.1016/j.heliyon.2019.e01431

Details

Primary Language

English

Subjects

Plant Biochemistry, Microbiology (Other)

Journal Section

Research Article

Early Pub Date

August 6, 2024

Publication Date

August 29, 2024

Submission Date

January 24, 2024

Acceptance Date

April 20, 2024

Published in Issue

Year 2024 Volume: 11 Number: 3

APA
Sümengen Özdenefe, M., Büyükkaya Kayış, F., Erol, Ü. H., & Mercimek Takcı, A. (2024). Phytochemical screening and in vitro biological activity of Amaranthus viridis growing in Northern Cyprus. International Journal of Secondary Metabolite, 11(3), 592-603. https://izlik.org/JA28RC66TG
AMA
1.Sümengen Özdenefe M, Büyükkaya Kayış F, Erol ÜH, Mercimek Takcı A. Phytochemical screening and in vitro biological activity of Amaranthus viridis growing in Northern Cyprus. Int. J. Sec. Metabolite. 2024;11(3):592-603. https://izlik.org/JA28RC66TG
Chicago
Sümengen Özdenefe, Melis, Fikret Büyükkaya Kayış, Ümit Haydar Erol, and Aysun Mercimek Takcı. 2024. “Phytochemical Screening and in Vitro Biological Activity of Amaranthus Viridis Growing in Northern Cyprus”. International Journal of Secondary Metabolite 11 (3): 592-603. https://izlik.org/JA28RC66TG.
EndNote
Sümengen Özdenefe M, Büyükkaya Kayış F, Erol ÜH, Mercimek Takcı A (August 1, 2024) Phytochemical screening and in vitro biological activity of Amaranthus viridis growing in Northern Cyprus. International Journal of Secondary Metabolite 11 3 592–603.
IEEE
[1]M. Sümengen Özdenefe, F. Büyükkaya Kayış, Ü. H. Erol, and A. Mercimek Takcı, “Phytochemical screening and in vitro biological activity of Amaranthus viridis growing in Northern Cyprus”, Int. J. Sec. Metabolite, vol. 11, no. 3, pp. 592–603, Aug. 2024, [Online]. Available: https://izlik.org/JA28RC66TG
ISNAD
Sümengen Özdenefe, Melis - Büyükkaya Kayış, Fikret - Erol, Ümit Haydar - Mercimek Takcı, Aysun. “Phytochemical Screening and in Vitro Biological Activity of Amaranthus Viridis Growing in Northern Cyprus”. International Journal of Secondary Metabolite 11/3 (August 1, 2024): 592-603. https://izlik.org/JA28RC66TG.
JAMA
1.Sümengen Özdenefe M, Büyükkaya Kayış F, Erol ÜH, Mercimek Takcı A. Phytochemical screening and in vitro biological activity of Amaranthus viridis growing in Northern Cyprus. Int. J. Sec. Metabolite. 2024;11:592–603.
MLA
Sümengen Özdenefe, Melis, et al. “Phytochemical Screening and in Vitro Biological Activity of Amaranthus Viridis Growing in Northern Cyprus”. International Journal of Secondary Metabolite, vol. 11, no. 3, Aug. 2024, pp. 592-03, https://izlik.org/JA28RC66TG.
Vancouver
1.Melis Sümengen Özdenefe, Fikret Büyükkaya Kayış, Ümit Haydar Erol, Aysun Mercimek Takcı. Phytochemical screening and in vitro biological activity of Amaranthus viridis growing in Northern Cyprus. Int. J. Sec. Metabolite [Internet]. 2024 Aug. 1;11(3):592-603. Available from: https://izlik.org/JA28RC66TG
International Journal of Secondary Metabolite

e-ISSN: 2148-6905