Bioactivity of secondary metabolite of endophytic fungi extract isolated from root of Jambu Mawar (Syzygium jambos (L.) Alston)

Abstract

This research aims to isolate endophytic fungi from Syzygium jambos and identify their active compounds. Endophytic fungi were isolated from the roots of S. jambos and cultured on Potato Dextrose Agar media. Antibacterial activity using the Kirby–Bauer method was tested on four Gram-positive and Gram-negative bacteria. Molecular identification was carried out on selected isolates to determine the species of endophytic fungi and isolate their active compounds. Column chromatography was used for compound isolation. The pure compounds were then analyzed spectroscopically using 1H-NMR, 13C-NMR, DEPT 135, HMQC, HMBC, COSY. The results of the isolation of endophytic fungi found four isolates SJR1 – 4, which show antibacterial activity. The strongest antibacterial activity was demonstrated by isolate SJR1, so it was continued with molecular identification. Molecular identification of SJR1 indicates that it is Lasiodiplodia iranensis. The pure compound L. iranensis was isolated and found to be 3-butyl-3,4-dihydroxy-6-((2-hydroxy-5-oxocyclopentyl) methyl)tetrahydro-pyran-2-one, which belongs to the phenolic group and has potential as an antibacterial. This compound can be used as an alternative medicinal ingredient.

Keywords

• Fungal endophyte
• Lasiodiplodia iranensis
• Secondary metabolites

Supporting Institution

the DRPM Ministry of Research and Technology of the Republic of Indonesia

Project Number

No. 057/E5/PG.02.00.PT/2022 and No. 0064.02/UN9.3.1/PL/2022

Thanks

Thank you to the DRPM Ministry of Research and Technology of the Republic of Indonesia for providing Doctoral Dissertation Grant funds, number: 2022, No. 057/E5/PG.02.00.PT/2022 and No. 0064.02/UN9.3.1/PL/2022

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