Research Article
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Comparison of volatile oil compositions of five Narcissus (Narcissus tazetta L.) varieties extracted

Year 2025, Volume: 12 Issue: 3, 498 - 511, 04.09.2025
https://doi.org/10.21448/ijsm.1509230

Abstract

Narcissus is one of the most important ornamental and medicinal plants, whose oil is widely used in the cosmetic, sanitary, and perfume industries. Khuzestan province, especially Behbahan district, is one of the most important Narcissus production centers in Iran. This research was conducted to evaluate and compare the volatile oil components of five Narcissus varieties (Shahla, Meskinack, Panjegorbai, Porpar, and Golsefied) which are commercially grown in Khuzestan Province. Enfleurage and head space solid-phase micro extraction methods were used for extraction. The fresh flowers of Narcissus were harvested and immediately transferred to the laboratory; the volatile oil was extracted by the enfleurage method. The components were analyzed by GC-MS and then the results were also compared against the headspace solid-phase micro extraction method. Trans-β-ocimene, benzyl acetate, linalool, 1.8-cineole, and 2-Phenyl ethyl acetate were the most common constituents which were identified in different varieties of Narcissus from both methods of extraction. According to the results of GC-MS, trans-β-ocimene was the dominant compound of the HS-SPME method while benzyl acetate was the dominant compound of the enfleurage method. The diverse varieties of Narcissus contain different components, therefore more research studies are recommended.

Supporting Institution

Shahid Chamran University of Ahvaz

Project Number

SCU.AH1400.775

Thanks

The authors of this research are thankful to Mr. Hasan Tajalli for supplying the narcissus flowers and facility encouragement. We are grateful to the research council of Shahid Chamran University of Ahvaz for financial support with grant number (SCU.AH1400.775).

References

  • Adams, R.P. (2017). Identification of essential oil components by gas chromatography / quadrupole mass spectrometry. 4th Edition. Allured Publ., Carol Stream, IL. 809 pp.
  • Al-Snafi, A.E. (2020). Constituents and pharmacology of Narcissus tazetta. International Organization of Scientific Research, Journal of Pharmacy, 10(9), 44-53.
  • Baranauskien, R., & Venskutonis, P.R. (2022). Supercritical CO2 extraction of Narcissus poeticus L. flowers for the isolation of volatile fragrance compounds. Molecules, 27(353), 1-20. https://doi.org/10.3390/molecules27020353
  • Chen, H.C., Chi, H.S., & Lin, L.Y. (2013). Headspace solid-phase microextraction analysis of volatile components in Narcissus tazetta var. chinensis Roem. Journal of Molecules, 18(1), 3723-13734. Doi: 10.3390/molecules181113723
  • Chehrazi, M., Naderi, R., Shahnejat Booshehri, A.A., & Hassani, M.E. (2007). Study of genetic diversity of exotic and endemic daffodils (Narcissus spp.) using RAPD markers. Iranian Journal of Horticulture Science and Technology, 8(4), 225–236. [In Persian]
  • Claveria, L.T., Tallini, L., Viladomat, F., & Bastida, J. (2017). Amaryllidaceae ornamental plants as sources of bioactive compounds. Recent Advances in Pharmaceutical Sciences, 7, 69-82.
  • Daghbouche, S., Ammar, I., Rekik, D.M., Djazouli, Z.E., Zebib, B., & Merah, O. (2020). Effect of phonological stages on essential oil composition of Cytisus triflorus L Her. Journal of King Saud University–Science, 32, 2383-2387. https://doi.org/10.1016/j.jksus.2020.03.020
  • Diaz-Maroto, M.C., Perez-Coello, M.S., & Cabezudo, M.D. (2006). Volatile components of Narcissus tazetta L. cv. Chinese sacred lily from Spain. Journal of Essential Oil Research, 18(6), 635-637. https://doi 10.1080/10412905.2006.9699314
  • Dobson, H., Arroyo, J., Bergestrom, G., & Groth, I. (1997). Interspecific variation in floral fragrances within the genus Narcissus (Amaryllidaceae). Journal of Biochemical Systematics and Ecology, 25(8), 685–706. https://doi.org/10.1016/S0305-1978(97)00059-8
  • Demir, N., Dasdemir, S.N., & Kaplan, A. (2021). Biochemica ınvestigation of the pharmaceutical and cosmetic use of Narcissus (Narcissus tazetta L. subsp. tazetta L.) growing naturally around ın Mugla, Turkey. Middle East Journal of Science, 7(1), 46-55. https://doi.org/10.51477/mejs.933062
  • Dourr, S., Dehghani Bidgoli, R., Akhbari, M., & Razmjoue, D. (2020). Evaluation and comparison of phenolic compounds and antioxidant activity extract of several population of Narcissus sp. from Behbahan city. Journal of New Cell Molecule Biotechnology, 10(37), 89–107. [In Persian]
  • Drew, D.P., Rasmussen, S.K., Avato, P., & Simonsen, H.T. (2012). A comparison of headspace solid-phase micro extraction and classic hydro distillation for the identification of volatile constituents from Thapsia spp. provides insights into guaianolide biosynthesis in Apiaceae. Phytochemical Analysis, 23, 44-51. https://doi: 10.1002/pca.1323
  • Ehret, C., Maupetit, P., & Petrzilka, M. (1992). New organoleptically important components of Narcissus absolute (Narcissus poeticus L.). Journal of Essential Oil Research, 4(1), 41-47. http://dx.doi.org/10.1080/10412905.1992.9698008
  • Ferri, D., Adami, M., De Santis, A., & Ubaldi, C., (2009). Traditional and supercritical CO2extraction of the volatile from Narcissus poeticus L. Perfumer and Flavorist, 34(9), 30–35.
  • Flamini, G., Cioni, P.L., & Morelli, I. (2003). Differences in the fragrances of pollen, leaves, and floral parts of garland (Chrysanthemum coronarium) and composition of the essential oils from flower heads and leaves. Journal of Agricultural and Food Chemistry, 51, 2267-2271. https://doi.org/10.1021/jf021050l
  • Handa, S.S., Singh, S.P., Longo, K.G., & Rakesh, D.D. (2008). Extraction technologies for medicinal and aromatic plants. International Centre for Science and High Technology, p. 34.
  • Hesham, H.A., Abdurahman, H.N., & Rasoli, M.Y. (2016). Techniques for extraction of essential oils from plants: A review. Australian Journal of Basic and Applied Sciences, 10(16), 117-127.
  • Koksal, N., Kafkas, E., Sadighazadi, S., & Kulahlioglu, I. (2015). Floral fragrances of daffodil under salinity stress. Romanian Biotechnological Letters, 20(4), 10600-10610.
  • Li, X., Tang, D., & Shi, Y. (2018). Volatile compounds in perianth and corona of Narcissus Pseudo Narcissus cultivars. Natural Product Research, 25, 277 284. https://doi.org/10.1080/14786419.2018.1499632
  • Li, Z.-G., Lee, M.R., & Shen, D.-L. (2006). Analysis of volatile compounds emitted from fresh Syringa oblata flowers in different florescence by headspace solid-phase micro extraction–gas chromatography–mass spectrometry. Analytical Chemical Acta, 576(1), 43-49. https://doi:10.1016/j.aca.2006.01.074
  • Losch, F., Liedtke, S., Vautz, W., & Weigend, M. (2023). Evaluation of floral volatile patterns in the genus Narcissus using gas chromatography–coupled ion mobility spectrometry. Applications in Plant Science, 11(1), 1-12. https://doi.org/10.1002/aps3.11506
  • Melliou, E., Kalpoutzakis, E., Tsitsa, E., & Magiatis, P. (2007). Composition of the essential oils of Narcissus tazetta and Narcissus serotinus from Greece. Journal of Essential Oil Bearing Plants, 10(2), 101-103. http://dx.doi.org/10.1080/0972060X.2007.10643526
  • Mishra, P., Rajbhar, S., & Devi, A. (2023). Narcissus Tazetta Leaves: A review of phytochemistry and potential therapeutic applications. International Journal of Pharmaceutical Research and Applications, 8(3), 3395-3406.
  • Padumanonda, T., Chayan, P., & Pensuk, W. (2007). The development of local animal fat as an alternative method in the extraction of essential oil for aromatherapy. Journal of Thai Traditional & Alternative Medicine, 5(3), 149 -156.
  • Paibon, W., Yimnoi, C.A., Tembab, N., Boonlue, W., Jampachaisri, K., Nuengchamnong, N., Waranuch, N., & Ingkaninan, K. (2011). Comparison and evaluation of volatile oils from three different extraction methods for some Thai fragrant flowers. International Journal of Cosmetic Science, 33, 150-156. http:// doi: 10.1111/j.1468-2494.2010.00603
  • Prakash, O., Rout, P.K., Chanotiya, C.S., & Misra, L.N. (2012). Composition of essential oil, concrete, absolute and SPME analysis of Tagetes patula capitula. Industrial Crops and Products, 37, 195-199. https://doi.org/10.1016/j.indcrop.2011.11.020
  • Ruíz-Ramón, F., Aquila, D.J., Covtines, M.E., & Weiss, J. (2014). Optimization of fragrance extraction: Daytime and flower age affect scent emission in simple and double Narcissus. Industrial Crops and Products, 52, 671 678. http://dx.doi.org/10.1016/j.indcrop.2013.11.034
  • Sharmeen, J.B., Mahomoodally, F.M., Zengin, K., & Maggi, F. (2021). Essential oils as natural sources of fragrance compounds for cosmetics and cosmeceuticals. Journal of Molecules, 26(666), 2-23. https://doi: 10.3390/molecules26030666
  • Song, G., Xiao, J., Deng, C., Zhang, X., & Hu, Y. (2007). Use of solid-phase micro extraction as a sampling technique for the characterization of volatile compounds emitted from Chinese daffodil flowers. Journal of Analytical Chemistry, 62(7), 674 679. https://doi.10.1134/S1061934807070118
  • Terry, M.I., Hernandez, V.R., Aquila, D.J., Weiss, J., & Cortines, M.E. (2021). The effect of post-harvest conditions in Narcissus sp. cut flowers scent profile. Journal of Frontiers in Plant Science, 11, 1-14. https://doi.10.3389/fpls.2020.540821
  • Van Dort, H.M., Jagers, P.P., ter Heide, R., & Van der Weerdt, A.J.A. (1993). Narcissus trevithian and Narcissus geranium: Analysis and synthesis of compounds. Journal of Agriculture and Food Chemistry, 41, 2063-2075. https://doi.org/10.1021/jf00035a047
  • Zarifikhosroshahi, M., Alp, S., & Kafkas, N.E. (2021). Characterization of aroma compounds of Daffodil (Narcissus tazetta L.) ecotypes from Turkey. International Journal of Agriculture, Forestry and Life Sciences, 5(1), 101-105. http://dergipark.gov.tr/ijafls
  • Zhu, F., Xu, J., Ke, Y., Huang, S., Zeng, F., Luan, T., & ouyang, G. (2013). Applications of in vivo and in vitro solid-phase micro extraction techniques in plant analysis. Analytical Chemical Acta, 794, 1-14. http://dx.doi.org/10.1016/j.aca.2013.05.016

Comparison of volatile oil compositions of five Narcissus (Narcissus tazetta L.) varieties extracted

Year 2025, Volume: 12 Issue: 3, 498 - 511, 04.09.2025
https://doi.org/10.21448/ijsm.1509230

Abstract

Narcissus is one of the most important ornamental and medicinal plants, whose oil is widely used in the cosmetic, sanitary, and perfume industries. Khuzestan province, especially Behbahan district, is one of the most important Narcissus production centers in Iran. This research was conducted to evaluate and compare the volatile oil components of five Narcissus varieties (Shahla, Meskinack, Panjegorbai, Porpar, and Golsefied) which are commercially grown in Khuzestan Province. Enfleurage and head space solid-phase micro extraction methods were used for extraction. The fresh flowers of Narcissus were harvested and immediately transferred to the laboratory; the volatile oil was extracted by the enfleurage method. The components were analyzed by GC-MS and then the results were also compared against the headspace solid-phase micro extraction method. Trans-β-ocimene, benzyl acetate, linalool, 1.8-cineole, and 2-Phenyl ethyl acetate were the most common constituents which were identified in different varieties of Narcissus from both methods of extraction. According to the results of GC-MS, trans-β-ocimene was the dominant compound of the HS-SPME method while benzyl acetate was the dominant compound of the enfleurage method. The diverse varieties of Narcissus contain different components, therefore more research studies are recommended.

Supporting Institution

Shahid Chamran University of Ahvaz

Project Number

SCU.AH1400.775

Thanks

The authors of this research are thankful to Mr. Hasan Tajalli for supplying the narcissus flowers and facility encouragement. We are grateful to the research council of Shahid Chamran University of Ahvaz for financial support with grant number (SCU.AH1400.775).

References

  • Adams, R.P. (2017). Identification of essential oil components by gas chromatography / quadrupole mass spectrometry. 4th Edition. Allured Publ., Carol Stream, IL. 809 pp.
  • Al-Snafi, A.E. (2020). Constituents and pharmacology of Narcissus tazetta. International Organization of Scientific Research, Journal of Pharmacy, 10(9), 44-53.
  • Baranauskien, R., & Venskutonis, P.R. (2022). Supercritical CO2 extraction of Narcissus poeticus L. flowers for the isolation of volatile fragrance compounds. Molecules, 27(353), 1-20. https://doi.org/10.3390/molecules27020353
  • Chen, H.C., Chi, H.S., & Lin, L.Y. (2013). Headspace solid-phase microextraction analysis of volatile components in Narcissus tazetta var. chinensis Roem. Journal of Molecules, 18(1), 3723-13734. Doi: 10.3390/molecules181113723
  • Chehrazi, M., Naderi, R., Shahnejat Booshehri, A.A., & Hassani, M.E. (2007). Study of genetic diversity of exotic and endemic daffodils (Narcissus spp.) using RAPD markers. Iranian Journal of Horticulture Science and Technology, 8(4), 225–236. [In Persian]
  • Claveria, L.T., Tallini, L., Viladomat, F., & Bastida, J. (2017). Amaryllidaceae ornamental plants as sources of bioactive compounds. Recent Advances in Pharmaceutical Sciences, 7, 69-82.
  • Daghbouche, S., Ammar, I., Rekik, D.M., Djazouli, Z.E., Zebib, B., & Merah, O. (2020). Effect of phonological stages on essential oil composition of Cytisus triflorus L Her. Journal of King Saud University–Science, 32, 2383-2387. https://doi.org/10.1016/j.jksus.2020.03.020
  • Diaz-Maroto, M.C., Perez-Coello, M.S., & Cabezudo, M.D. (2006). Volatile components of Narcissus tazetta L. cv. Chinese sacred lily from Spain. Journal of Essential Oil Research, 18(6), 635-637. https://doi 10.1080/10412905.2006.9699314
  • Dobson, H., Arroyo, J., Bergestrom, G., & Groth, I. (1997). Interspecific variation in floral fragrances within the genus Narcissus (Amaryllidaceae). Journal of Biochemical Systematics and Ecology, 25(8), 685–706. https://doi.org/10.1016/S0305-1978(97)00059-8
  • Demir, N., Dasdemir, S.N., & Kaplan, A. (2021). Biochemica ınvestigation of the pharmaceutical and cosmetic use of Narcissus (Narcissus tazetta L. subsp. tazetta L.) growing naturally around ın Mugla, Turkey. Middle East Journal of Science, 7(1), 46-55. https://doi.org/10.51477/mejs.933062
  • Dourr, S., Dehghani Bidgoli, R., Akhbari, M., & Razmjoue, D. (2020). Evaluation and comparison of phenolic compounds and antioxidant activity extract of several population of Narcissus sp. from Behbahan city. Journal of New Cell Molecule Biotechnology, 10(37), 89–107. [In Persian]
  • Drew, D.P., Rasmussen, S.K., Avato, P., & Simonsen, H.T. (2012). A comparison of headspace solid-phase micro extraction and classic hydro distillation for the identification of volatile constituents from Thapsia spp. provides insights into guaianolide biosynthesis in Apiaceae. Phytochemical Analysis, 23, 44-51. https://doi: 10.1002/pca.1323
  • Ehret, C., Maupetit, P., & Petrzilka, M. (1992). New organoleptically important components of Narcissus absolute (Narcissus poeticus L.). Journal of Essential Oil Research, 4(1), 41-47. http://dx.doi.org/10.1080/10412905.1992.9698008
  • Ferri, D., Adami, M., De Santis, A., & Ubaldi, C., (2009). Traditional and supercritical CO2extraction of the volatile from Narcissus poeticus L. Perfumer and Flavorist, 34(9), 30–35.
  • Flamini, G., Cioni, P.L., & Morelli, I. (2003). Differences in the fragrances of pollen, leaves, and floral parts of garland (Chrysanthemum coronarium) and composition of the essential oils from flower heads and leaves. Journal of Agricultural and Food Chemistry, 51, 2267-2271. https://doi.org/10.1021/jf021050l
  • Handa, S.S., Singh, S.P., Longo, K.G., & Rakesh, D.D. (2008). Extraction technologies for medicinal and aromatic plants. International Centre for Science and High Technology, p. 34.
  • Hesham, H.A., Abdurahman, H.N., & Rasoli, M.Y. (2016). Techniques for extraction of essential oils from plants: A review. Australian Journal of Basic and Applied Sciences, 10(16), 117-127.
  • Koksal, N., Kafkas, E., Sadighazadi, S., & Kulahlioglu, I. (2015). Floral fragrances of daffodil under salinity stress. Romanian Biotechnological Letters, 20(4), 10600-10610.
  • Li, X., Tang, D., & Shi, Y. (2018). Volatile compounds in perianth and corona of Narcissus Pseudo Narcissus cultivars. Natural Product Research, 25, 277 284. https://doi.org/10.1080/14786419.2018.1499632
  • Li, Z.-G., Lee, M.R., & Shen, D.-L. (2006). Analysis of volatile compounds emitted from fresh Syringa oblata flowers in different florescence by headspace solid-phase micro extraction–gas chromatography–mass spectrometry. Analytical Chemical Acta, 576(1), 43-49. https://doi:10.1016/j.aca.2006.01.074
  • Losch, F., Liedtke, S., Vautz, W., & Weigend, M. (2023). Evaluation of floral volatile patterns in the genus Narcissus using gas chromatography–coupled ion mobility spectrometry. Applications in Plant Science, 11(1), 1-12. https://doi.org/10.1002/aps3.11506
  • Melliou, E., Kalpoutzakis, E., Tsitsa, E., & Magiatis, P. (2007). Composition of the essential oils of Narcissus tazetta and Narcissus serotinus from Greece. Journal of Essential Oil Bearing Plants, 10(2), 101-103. http://dx.doi.org/10.1080/0972060X.2007.10643526
  • Mishra, P., Rajbhar, S., & Devi, A. (2023). Narcissus Tazetta Leaves: A review of phytochemistry and potential therapeutic applications. International Journal of Pharmaceutical Research and Applications, 8(3), 3395-3406.
  • Padumanonda, T., Chayan, P., & Pensuk, W. (2007). The development of local animal fat as an alternative method in the extraction of essential oil for aromatherapy. Journal of Thai Traditional & Alternative Medicine, 5(3), 149 -156.
  • Paibon, W., Yimnoi, C.A., Tembab, N., Boonlue, W., Jampachaisri, K., Nuengchamnong, N., Waranuch, N., & Ingkaninan, K. (2011). Comparison and evaluation of volatile oils from three different extraction methods for some Thai fragrant flowers. International Journal of Cosmetic Science, 33, 150-156. http:// doi: 10.1111/j.1468-2494.2010.00603
  • Prakash, O., Rout, P.K., Chanotiya, C.S., & Misra, L.N. (2012). Composition of essential oil, concrete, absolute and SPME analysis of Tagetes patula capitula. Industrial Crops and Products, 37, 195-199. https://doi.org/10.1016/j.indcrop.2011.11.020
  • Ruíz-Ramón, F., Aquila, D.J., Covtines, M.E., & Weiss, J. (2014). Optimization of fragrance extraction: Daytime and flower age affect scent emission in simple and double Narcissus. Industrial Crops and Products, 52, 671 678. http://dx.doi.org/10.1016/j.indcrop.2013.11.034
  • Sharmeen, J.B., Mahomoodally, F.M., Zengin, K., & Maggi, F. (2021). Essential oils as natural sources of fragrance compounds for cosmetics and cosmeceuticals. Journal of Molecules, 26(666), 2-23. https://doi: 10.3390/molecules26030666
  • Song, G., Xiao, J., Deng, C., Zhang, X., & Hu, Y. (2007). Use of solid-phase micro extraction as a sampling technique for the characterization of volatile compounds emitted from Chinese daffodil flowers. Journal of Analytical Chemistry, 62(7), 674 679. https://doi.10.1134/S1061934807070118
  • Terry, M.I., Hernandez, V.R., Aquila, D.J., Weiss, J., & Cortines, M.E. (2021). The effect of post-harvest conditions in Narcissus sp. cut flowers scent profile. Journal of Frontiers in Plant Science, 11, 1-14. https://doi.10.3389/fpls.2020.540821
  • Van Dort, H.M., Jagers, P.P., ter Heide, R., & Van der Weerdt, A.J.A. (1993). Narcissus trevithian and Narcissus geranium: Analysis and synthesis of compounds. Journal of Agriculture and Food Chemistry, 41, 2063-2075. https://doi.org/10.1021/jf00035a047
  • Zarifikhosroshahi, M., Alp, S., & Kafkas, N.E. (2021). Characterization of aroma compounds of Daffodil (Narcissus tazetta L.) ecotypes from Turkey. International Journal of Agriculture, Forestry and Life Sciences, 5(1), 101-105. http://dergipark.gov.tr/ijafls
  • Zhu, F., Xu, J., Ke, Y., Huang, S., Zeng, F., Luan, T., & ouyang, G. (2013). Applications of in vivo and in vitro solid-phase micro extraction techniques in plant analysis. Analytical Chemical Acta, 794, 1-14. http://dx.doi.org/10.1016/j.aca.2013.05.016
There are 33 citations in total.

Details

Primary Language English
Subjects Plant Biochemistry
Journal Section Research Article
Authors

Mohammad Younis Mahen 0000-0001-5980-8440

Mohammad Mahmoodi Sourestani 0000-0002-0298-4918

Hosien Motamedi This is me 0000-0003-0310-7017

Seeyed Mansoor Seeyednejad This is me 0000-0001-6355-5941

Project Number SCU.AH1400.775
Submission Date July 2, 2024
Acceptance Date November 16, 2024
Early Pub Date June 11, 2025
Publication Date September 4, 2025
Published in Issue Year 2025 Volume: 12 Issue: 3

Cite

APA Mahen, M. Y., Mahmoodi Sourestani, M., Motamedi, H., Seeyednejad, S. M. (2025). Comparison of volatile oil compositions of five Narcissus (Narcissus tazetta L.) varieties extracted. International Journal of Secondary Metabolite, 12(3), 498-511. https://doi.org/10.21448/ijsm.1509230
International Journal of Secondary Metabolite

e-ISSN: 2148-6905