Investigation of antimicrobial effects of essential oil and secondary metabolite extraction from Ruscus aculeatus L. (Asparagaceae)

Ali Soydinç; Rasim Aktürk; Seda Biryol; Mehtap Usta

doi:10.21448/ijsm.1629765

Investigation of antimicrobial effects of essential oil and secondary metabolite extraction from Ruscus aculeatus L. (Asparagaceae)

Abstract

Ruscus aculeatus L. (Asparagaceae) is an important species with medicinal, industrial, and ornamental uses. In this study, the oil extract and secondary metabolites obtained from the fruit of R. aculeatus were tested against some bacterial and fungal pathogens to investigate their antimicrobial properties. R. aculeatus plant was collected from Yalıncak/Trabzon in May 2024. To get essential oil, the ripe fruits of the R. aculeatus plant dry weight were weighed, crushed with a mortar and transferred to a Soxhlet cartridge. And for getting secondary metabolites, 30 grams of ground leaves were placed in two different jars containing 100 mL of ethanol and methanol. The crude extracts and the solvents were evaporated in a rotary evaporator to obtain crude extracts. The extract obtained was used for antimicrobial tests by the disc diffusion method. Firstly, each pathogen was inoculated into Mueller-Hinton Agar (MHA) medium by smear inoculation. Then, antibiotic discs were placed in the medium. Each disc was impregnated with 30 µl of extract. The same volume of kanamycin was used as a control. After 24 and 48 hours, the diameters of the zones formed around the discs were measured and recorded. In the antimicrobial experiment, the analysis of the ES column revealed that the compound demonstrated no efficacy against E. cloaceae, S. epidermidis, K pneumoniae, and C. albicans (p>0.01). However, it was observed to form a significant inhibition zone in other bacterial groups. At the end of the study, it was determined that the extraction was effective on Bacillus subtilis ATCC 6633.

Keywords

Thanks

We would like to thank to Trabzon Regional Directorate of Forestry and Biological Control Laboratory against Forest Pests for making use of the infrastructure facilities.

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Details

Primary Language

English

Subjects

Microbiology (Other)

Journal Section

Research Article

Authors

Ali Soydinç
0000-0002-1032-1807
Türkiye

Rasim Aktürk This is me
0009-0005-7037-9559
Türkiye

Seda Biryol
0000-0003-0881-5004
Türkiye

Mehtap Usta ^*
0000-0001-7656-5655
Türkiye

Early Pub Date

September 1, 2025

Publication Date

December 5, 2025

Submission Date

January 30, 2025

Acceptance Date

June 21, 2025

Published in Issue

Year 2025 Volume: 12 Number: 4

DOI

https://doi.org/10.21448/ijsm.1629765

IZ

https://izlik.org/JA83YZ23CH

Cite

RIS / Bibtex

APA

Soydinç, A., Aktürk, R., Biryol, S., & Usta, M. (2025). Investigation of antimicrobial effects of essential oil and secondary metabolite extraction from Ruscus aculeatus L. (Asparagaceae). International Journal of Secondary Metabolite, 12(4), 832-846. https://doi.org/10.21448/ijsm.1629765

AMA

1.Soydinç A, Aktürk R, Biryol S, Usta M. Investigation of antimicrobial effects of essential oil and secondary metabolite extraction from Ruscus aculeatus L. (Asparagaceae). Int. J. Sec. Metabolite. 2025;12(4):832-846. doi:10.21448/ijsm.1629765

Chicago

Soydinç, Ali, Rasim Aktürk, Seda Biryol, and Mehtap Usta. 2025. “Investigation of Antimicrobial Effects of Essential Oil and Secondary Metabolite Extraction from Ruscus Aculeatus L. (Asparagaceae)”. International Journal of Secondary Metabolite 12 (4): 832-46. https://doi.org/10.21448/ijsm.1629765.

EndNote

Soydinç A, Aktürk R, Biryol S, Usta M (December 1, 2025) Investigation of antimicrobial effects of essential oil and secondary metabolite extraction from Ruscus aculeatus L. (Asparagaceae). International Journal of Secondary Metabolite 12 4 832–846.

IEEE

[1]A. Soydinç, R. Aktürk, S. Biryol, and M. Usta, “Investigation of antimicrobial effects of essential oil and secondary metabolite extraction from Ruscus aculeatus L. (Asparagaceae)”, Int. J. Sec. Metabolite, vol. 12, no. 4, pp. 832–846, Dec. 2025, doi: 10.21448/ijsm.1629765.

ISNAD

Soydinç, Ali - Aktürk, Rasim - Biryol, Seda - Usta, Mehtap. “Investigation of Antimicrobial Effects of Essential Oil and Secondary Metabolite Extraction from Ruscus Aculeatus L. (Asparagaceae)”. International Journal of Secondary Metabolite 12/4 (December 1, 2025): 832-846. https://doi.org/10.21448/ijsm.1629765.

JAMA

1.Soydinç A, Aktürk R, Biryol S, Usta M. Investigation of antimicrobial effects of essential oil and secondary metabolite extraction from Ruscus aculeatus L. (Asparagaceae). Int. J. Sec. Metabolite. 2025;12:832–846.

MLA

Soydinç, Ali, et al. “Investigation of Antimicrobial Effects of Essential Oil and Secondary Metabolite Extraction from Ruscus Aculeatus L. (Asparagaceae)”. International Journal of Secondary Metabolite, vol. 12, no. 4, Dec. 2025, pp. 832-46, doi:10.21448/ijsm.1629765.

Vancouver

1.Ali Soydinç, Rasim Aktürk, Seda Biryol, Mehtap Usta. Investigation of antimicrobial effects of essential oil and secondary metabolite extraction from Ruscus aculeatus L. (Asparagaceae). Int. J. Sec. Metabolite. 2025 Dec. 1;12(4):832-46. doi:10.21448/ijsm.1629765