Effects of biogenic silver nanoparticles applied to Nepeta cataria L. seeds on sterilization and germination

Burcu Çetin; Çiğdem Ay; Aslı Aktay

doi:10.21448/ijsm.1699267

Effects of biogenic silver nanoparticles applied to Nepeta cataria L. seeds on sterilization and germination

Abstract

Plant tissue culture applications are carried out under sterile conditions. Culture media must be created without biological contamination and must be maintained aseptically. Contaminations that occur in the culture media may affect experimental results by preventing adequate nutrition and the development of plants. Contamination is mostly caused by microorganisms found on the surfaces of plant tissues used in culture processes. For this reason, explants must be subjected to sterilization processes before culture processes. However, sterilants used for this purpose may have toxic effects on plant tissues, and in recent years, there has been a need to discover effective sterilants that do not show toxic effects. This study aimed to determine the potential of silver nanoparticles (AgNP) in surface sterilization processes for sterile in vitro germination of Nepeta cataria L. seeds. In order to determine the most appropriate concentration and time to be applied to plant seeds, five different concentrations (0, 75, 100, 125, 150 mg/L) and three different times (5, 10, 20 min) were tested. As a result of the applications, it was determined that the lowest contamination was in the seeds that were kept in 70% ethyl alcohol for 3 min and then in AgNP solutions at a concentration of 150 mg/L for 20 min. No toxicity symptoms were observed in the plants obtained. The results show that AgNPs can be used to obtain in vitro sterile plants.

Keywords

References

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Details

Primary Language

English

Subjects

Plant Biochemistry, Plant Biotechnology, Microbiology (Other)

Journal Section

Research Article

Authors

Burcu Çetin ^*
0000-0003-1465-1464
Türkiye

Çiğdem Ay
0000-0002-8283-0678
Türkiye

Aslı Aktay This is me
0009-0006-5058-7198
Türkiye

Early Pub Date

September 1, 2025

Publication Date

December 5, 2025

Submission Date

May 14, 2025

Acceptance Date

July 8, 2025

Published in Issue

Year 2025 Volume: 12 Number: 4

DOI

https://doi.org/10.21448/ijsm.1699267

IZ

https://izlik.org/JA45MW46TP

Cite

RIS / Bibtex

APA

Çetin, B., Ay, Ç., & Aktay, A. (2025). Effects of biogenic silver nanoparticles applied to Nepeta cataria L. seeds on sterilization and germination. International Journal of Secondary Metabolite, 12(4), 799-807. https://doi.org/10.21448/ijsm.1699267

AMA

1.Çetin B, Ay Ç, Aktay A. Effects of biogenic silver nanoparticles applied to Nepeta cataria L. seeds on sterilization and germination. Int. J. Sec. Metabolite. 2025;12(4):799-807. doi:10.21448/ijsm.1699267

Chicago

Çetin, Burcu, Çiğdem Ay, and Aslı Aktay. 2025. “Effects of Biogenic Silver Nanoparticles Applied to Nepeta Cataria L. Seeds on Sterilization and Germination”. International Journal of Secondary Metabolite 12 (4): 799-807. https://doi.org/10.21448/ijsm.1699267.

EndNote

Çetin B, Ay Ç, Aktay A (December 1, 2025) Effects of biogenic silver nanoparticles applied to Nepeta cataria L. seeds on sterilization and germination. International Journal of Secondary Metabolite 12 4 799–807.

IEEE

[1]B. Çetin, Ç. Ay, and A. Aktay, “Effects of biogenic silver nanoparticles applied to Nepeta cataria L. seeds on sterilization and germination”, Int. J. Sec. Metabolite, vol. 12, no. 4, pp. 799–807, Dec. 2025, doi: 10.21448/ijsm.1699267.

ISNAD

Çetin, Burcu - Ay, Çiğdem - Aktay, Aslı. “Effects of Biogenic Silver Nanoparticles Applied to Nepeta Cataria L. Seeds on Sterilization and Germination”. International Journal of Secondary Metabolite 12/4 (December 1, 2025): 799-807. https://doi.org/10.21448/ijsm.1699267.

JAMA

1.Çetin B, Ay Ç, Aktay A. Effects of biogenic silver nanoparticles applied to Nepeta cataria L. seeds on sterilization and germination. Int. J. Sec. Metabolite. 2025;12:799–807.

MLA

Çetin, Burcu, et al. “Effects of Biogenic Silver Nanoparticles Applied to Nepeta Cataria L. Seeds on Sterilization and Germination”. International Journal of Secondary Metabolite, vol. 12, no. 4, Dec. 2025, pp. 799-07, doi:10.21448/ijsm.1699267.

Vancouver

1.Burcu Çetin, Çiğdem Ay, Aslı Aktay. Effects of biogenic silver nanoparticles applied to Nepeta cataria L. seeds on sterilization and germination. Int. J. Sec. Metabolite. 2025 Dec. 1;12(4):799-807. doi:10.21448/ijsm.1699267

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