Multitarget biological activities and RP-HPLC-Based phytochemical profiling of Glaucium leiocarpum Boiss.

Sevgi Altın

doi:10.21448/ijsm.1768134

Multitarget biological activities and RP-HPLC-Based phytochemical profiling of Glaucium leiocarpum Boiss.

Abstract

This study presents a comprehensive evaluation of the phenolic composition and biological activities of methanolic extracts obtained from the aerial (GLM) and root (GLRM) parts of Glaucium leiocarpum. Phenolic profiling was conducted using reversed-phase high-performance liquid chromatography (RP-HPLC), revealing catechin hydrate, 3-hydroxybenzoic acid, syringic acid, hesperidin, chlorogenic acid, caffeic acid, and trans-cinnamic acid as major constituents in GLM. In contrast, GLRM exhibited a more diverse phenolic profile, including epicatechin, benzoic acid, quercetin, gallic acid, and rosmarinic acid. Antioxidant activities were evaluated using DPPH and FRAP assays, with GLRM demonstrating stronger radical scavenging capacity (IC₅₀: 36.96 µg/mL) and higher ferric reducing power (173.20±0.68 mg TE/g extract). GLRM also contained significantly higher levels of total phenolics (142.81±2.87 mg GAE/g) and flavonoids (76.67±0.72 mg QE/g). Antimicrobial assays demonstrated that only GLRM exerted inhibitory effects against Staphylococcus aureus (8.7±0.57 mm), Enterococcus faecalis (7.7±0.57 mm), and Streptococcus pneumoniae (15.0±0.0 mm). Cytotoxicity studies, assessed by the XTT assay, revealed potent antiproliferative effects against HT-29 colorectal cancer cells (IC₅₀: 3.59 µg/mL for GLM, 2.29 µg/mL for GLRM) and moderate activity against A549 lung carcinoma cells (IC₅₀: 36.01 µg/mL for GLM, 21.99 µg/mL for GLRM). Furthermore, GLRM exhibited strong anticholinesterase activity, with IC₅₀ values of 2.85±0.27 µg/mL for acetylcholinesterase (AChE) and 2.47±0.07 µg/mL for butyrylcholinesterase (BChE). Overall, these findings underscore G. leiocarpum, particularly its root extract, as a valuable source of bioactive secondary metabolites, owing to its rich polyphenolic profile and notable antioxidant, antimicrobial, cytotoxic, and cholinesterase inhibitory activities.

Keywords

Thanks

The authors thank the Erzincan Binali Yıldırım University Scientific Research Center for providing the necessary equipment for cell culture studies and the Aksaray University Science and Technology Application and Research Center for providing the necessary equipment for RP-HPLC analysis.

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Details

Primary Language

English

Subjects

Natural Products and Bioactive Compounds

Journal Section

Research Article

Authors

Sevgi Altın ^*
0000-0002-0820-912X
Türkiye

Early Pub Date

August 4, 2026

Publication Date

September 2, 2026

Submission Date

August 20, 2025

Acceptance Date

May 23, 2026

Published in Issue

Year 2026 Volume: 13 Number: 3

DOI

https://doi.org/10.21448/ijsm.1768134

IZ

https://izlik.org/JA92TE59CA

Cite

RIS / Bibtex

APA

Altın, S. (2026). Multitarget biological activities and RP-HPLC-Based phytochemical profiling of Glaucium leiocarpum Boiss. International Journal of Secondary Metabolite, 13(3), 861-877. https://doi.org/10.21448/ijsm.1768134

AMA

1.Altın S. Multitarget biological activities and RP-HPLC-Based phytochemical profiling of Glaucium leiocarpum Boiss. Int. J. Sec. Metabolite. 2026;13(3):861-877. doi:10.21448/ijsm.1768134

Chicago

Altın, Sevgi. 2026. “Multitarget Biological Activities and RP-HPLC-Based Phytochemical Profiling of Glaucium Leiocarpum Boiss”. International Journal of Secondary Metabolite 13 (3): 861-77. https://doi.org/10.21448/ijsm.1768134.

EndNote

Altın S (September 1, 2026) Multitarget biological activities and RP-HPLC-Based phytochemical profiling of Glaucium leiocarpum Boiss. International Journal of Secondary Metabolite 13 3 861–877.

IEEE

[1]S. Altın, “Multitarget biological activities and RP-HPLC-Based phytochemical profiling of Glaucium leiocarpum Boiss”., Int. J. Sec. Metabolite, vol. 13, no. 3, pp. 861–877, Sept. 2026, doi: 10.21448/ijsm.1768134.

ISNAD

Altın, Sevgi. “Multitarget Biological Activities and RP-HPLC-Based Phytochemical Profiling of Glaucium Leiocarpum Boiss”. International Journal of Secondary Metabolite 13/3 (September 1, 2026): 861-877. https://doi.org/10.21448/ijsm.1768134.

JAMA

1.Altın S. Multitarget biological activities and RP-HPLC-Based phytochemical profiling of Glaucium leiocarpum Boiss. Int. J. Sec. Metabolite. 2026;13:861–877.

MLA

Altın, Sevgi. “Multitarget Biological Activities and RP-HPLC-Based Phytochemical Profiling of Glaucium Leiocarpum Boiss”. International Journal of Secondary Metabolite, vol. 13, no. 3, Sept. 2026, pp. 861-77, doi:10.21448/ijsm.1768134.

Vancouver

1.Sevgi Altın. Multitarget biological activities and RP-HPLC-Based phytochemical profiling of Glaucium leiocarpum Boiss. Int. J. Sec. Metabolite. 2026 Sep. 1;13(3):861-77. doi:10.21448/ijsm.1768134