Phenolic Contents and Antioxidant Properties of Echinops ritro L. and E. tournefortii Jaup. Et. Spach Extract

Abstract

Aim of the study was to evaluate antioxidant activity and total phenolic content of Echinops ritro L. and E. tournefortii (Asteraceae). The dried leaves and seeds of E. ritro and E. tournefortii were extracted separately with ethanol, methanol, chloroform and dH₂O. Total phenolic content was measured by Folin-Ciocalteu method. Antioxidant activities of the extracts were determined by two test systems namely, radical scavenging on DPPH and β-carotene bleaching methods. dH₂O extracts has the highest phenolic content (92.24 GAE mg/100g). The results were compared to those of BHT as synthetic antioxidant. dH₂O extracts were found to be rich as a source of phenolics. According to the results of antioxidant activity, dH₂O extracts exhibited higher antioxidant activity than all types of solvent. The strongest antioxidant properties were obtained by dH₂O extract. Radical scavenging activities (%) were found to be in the following order: Chloroform<Ethanol<Methanol<dH₂O<BHT. These results of the present study demonstrated that the extracts of E. ritro and E. tournefortii may be used as a source of natural antioxidant in the food and pharmaceutical industries.

Keywords

• Echinops ritro
• E. tournefortii
• antioxidant activity
• phenolic content

Phenolic Contents and Antioxidant Properties of Echinops ritro L. and E. tournefortii Jaup. Et. Spach Extract

Abstract

Aim of the study was to evaluate antioxidant activity and total phenolic content of Echinops ritro L. and E. tournefortii (Asteraceae). The dried leaves and seeds of E. ritro and E. tournefortii were extracted separately with ethanol, methanol, chloroform and dH2O. Total phenolic content was measured by Folin-Ciocalteu method. Antioxidant activities of the extracts were determined by two test systems namely, radical scavenging on DPPH and β-carotene bleaching methods. dH2O extracts has the highest phenolic content (92.24 GAE mg/100g). The results were compared to those of BHT as synthetic antioxidant. dH2O extracts were found to be rich as a source of phenolics. According to the results of antioxidant activity, dH2O extracts exhibited higher antioxidant activity than all types of solvent. The strongest antioxidant properties were obtained by dH2O extract. Radical scavenging activities (%) were found to be in the following order: Chloroform

Keywords

• Echinops ritro
• E. tournefortii
• antioxidant activity
• phenolic content

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