Total Phenolics, Antioxidant, Antibacterial and Cytotoxic Activity Studies of Ethanolic Extracts Arisarum vulgare O.Targ.Tozz. and Dracunculus vulgaris Schott.

Abstract

The present study reports the antioxidant, total phenolics, antibacterial and cytotoxic activities of Arisarum vulgare and Dracunculus vulgaris of aerial and under ground ethanol extracts.  The antioxidant activity was determined by DPPH radical scavenging activity and β-carotene linoleic acid assays. Folin-Ciocalteu assay was used to detect total phenolic contents in the extract. In vitro cytotoxic activity was determined by the Brine shrimp lethality test. Antibacterial activity was investigated with the microdilution method. As a result, under ground parts of the extract showed higher antioxidant activity than aerial parts of the extract. Total phenolics ranged from 14.5±2.02 to 53.4±2.01 mg GAE/g, and the antioxidant activity according to the β-carotene/linoleic acid assay ranged from 70.76±1.08 % to 85.43±1.05 and according to the DPPH assay IC₅₀ values ranged from 0.089±1.02 to 1.095±1.07mg/ml. The A. vulgare under ground extract was tested against 2 and showed a good antibacterial activity at a concentration of <50 mg/mL Minimum inhibitory concentration (MIC) values for the bacteria S.aureus. The results of the cytotoxic activities showed a very high activity of the extracts, D.vulgaris under ground extract  (LC₅₀ = 10.6 µg/mL).

Keywords

• Antioxidant
• antibacterial
• cytotoxic
• Arisarum vulgare
• Dracunculus vulgaris

Total Phenolics, Antioxidant, Antibacterial and Cytotoxic Activity Studies of Ethanolic Extracts Arisarum vulgare O.Targ.Tozz. and Dracunculus vulgaris Schott.

Abstract

The present study reports the antioxidant, total phenolics, antibacterial and cytotoxic activities of Arisarum vulgare and Dracunculus vulgaris of aerial and under ground ethanol extracts. The antioxidant activity was determined by DPPH radical scavenging activity and β-carotene linoleic acid assays. Folin-Ciocalteu assay was used to detect total phenolic contents in the extract. In vitro cytotoxic activity was determined by the Brine shrimp lethality test. Antibacterial activity was investigated with the microdilution method. As a result, under ground parts of the extract showed higher antioxidant activity than aerial parts of the extract. Total phenolics ranged from 14.5±2.02 to 53.4±2.01 mg GAE/g, and the antioxidant activity according to the β-carotene/linoleic acid assay ranged from 70.76±1.08 % to 85.43±1.05 and according to the DPPH assay IC50 values ranged from 0.089±1.02 to 1.095±1.07mg/ml. The A. vulgare under ground extract was tested against 2 and showed a good antibacterial activity at a concentration of <50 mg/mL Minimum inhibitory concentration (MIC) values for the bacteria S.aureus. The results of the cytotoxic activities showed a very high activity of the extracts, D.vulgaris under ground extract (LC50 = 10.6 µg/mL).

Keywords

• Antioxidant
• antibacterial
• cytotoxic
• Arisarum vulgare
• Dracunculus vulgaris

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