Chemical Composition and Insecticidal Activity of Origanum syriacum L. Essential Oil Against Sitophilus oryzae and Rhyzopertha dominica

Abstract

Origanum genus belonging to the Lamiaceae family is aromatic and medicinal plant. It has been used in many countries for medicinal and pharmaceutical purpose. Aerial part of the Origanum syriacum L. was dried at shade. The essential oil was generated by steam distillation and compounds were identified by GC-MS analysis. γ-terpinene (26.7%), thymol (26.6%) and carvacrol (22.9%) was detected as the mail constituents. The essential oil was tested for insecticidal activity against adult of Sitophilus oryzae (L.) (Coleoptera: Curculionidae) and Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) using fumigation method. Essential oil revealed the excellent fumigant effect on R. dominica adults with a median lethal concentration (LC50) value of 0.124 µl/insect and 0.107 µl/insect for 48 h and 72 h respectively. LC50 values of S. oryzae were found as 0.173 µl/insect and 0.135 µl/insect for 48 h and 72 h respectively. As a result, essential oil of O. syriacum has a potency to be a natural insecticide.

Keywords

• Origanum syriacum
• Insecticidal activity
• Fumigant toxicity
• Essential oil

Chemical Composition and Insecticidal Activity of Origanum syriacum L. Essential Oil Against Sitophilus oryzae and Rhyzopertha dominica

Abstract

Origanum genus belonging to the Lamiaceae family is aromatic and medicinal plant. It has been used in many countries for medicinal and pharmaceutical purpose. Aerial part of the Origanum syriacum L. was dried at shade. The essential oil was generated by steam distillation and compounds were identified by GC-MS analysis. γ-terpinene (26.7%), thymol (26.6%) and carvacrol (22.9%) was detected as the mail constituents. The essential oil was tested for insecticidal activity against adult of Sitophilus oryzae (L.) (Coleoptera: Curculionidae) and Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) using fumigation method. Essential oil revealed the excellent fumigant effect on R. dominica adults with a median lethal concentration (LC₅₀) value of 0.124 µl/insect and 0.107 µl/insect for 48 h and 72 h respectively. LC₅₀ values of S. oryzae were found as 0.173 µl/insect and 0.135 µl/insect for 48 h and 72 h respectively. As a result, essential oil of O. syriacum has a potency to be a natural insecticide.

Keywords

• Origanum syriacum
• Insecticidal activity
• Fumigant toxicity
• Essential oil

References

1. Elmastas, M., Erenler, R., Isnac, B., Aksit, H., Sen, O., Genc, N., & Demirtas, I. (2016). Isolation and identification of a new neo-clerodane diterpenoid from Teucrium chamaedrys L. Natural Product Research, 30 (3): 299-304.
2. Erenler, R., Yilmaz, S., Aksit, H., Sen, O., Genc, N., Elmastas, M., & Demirtas, I. (2014). Antioxidant Activities of Chemical Constituents Isolated from Echinops orientalis Trauv. Records of Natural Products, 8 (1): 32-36.
3. Erenler, R., Sen, O., Yildiz, I., & Aydin, A. (2016). Antiproliferative activities of chemical constituents isolated from Thymus praecox subsp grossheimii (Ronniger) Jalas. Records of Natural Products, 10 (6), 766-770.
4. Demirtas, I., Erenler, R., Elmastas, M., & Goktasoglu, A. (2013). Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction. Food Chemistry, 136 (1), 34-40.
5. Davis, P.H. (1998). Flora of Turkey and the East Aegean Islands. Edinburg, United Kingdom: University Press.
6. Jun, W.J., Han, B.K., Yu, K.W., Kim, M.S., Chang, I.S., Kim, H.Y., & Cho, H.Y. (2001). Antioxidant effects of Origanum majorana L. on superoxide anion radicals. Food Chemistry, 75 (4): 439-444.
7. Erenler, R., Sen, O., Aksit, H., Demirtas, I., Yaglioglu, A.S., Elmastas, M., & Telci, I. (2016). Isolation and identification of chemical constituents from Origanum majorana and investigation of antiproliferative and antioxidant activities. Journal of the Science of Food and Agriculture, 96 (3): 822-836.
8. Erenler, R., Meral, B., Sen, O., Elmastas, M., Aydin, A., Eminagaoglu, O., & Topcu, G. (2017). Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities. Pharmaceutical Biology, 55 (1): 1646-1653.

9. Busatta, C., Vidal, R.S., Popiolski, A.S., Mossi, A.J., Dariva, C., Rodrigues, M.R.A., Corazza, F.C., Corazza, M.L., Oliveira, J.V., & Cansian, R.L. (2008). Application of Origanum majorana L. essential oil as an antimicrobial agent in sausage. Food Microbiology, 25 (1): 207-211.
10. Economou, G., Panagopoulos, G., Tarantilis, P., Kalivas, D., Kotoulas, V., Travlos, I.S., Polysiou, M., & Karamanos, A. (2011). Variability in essential oil content and composition of Origanum hirtum L., Origanum onites L., Coridothymus capitatus (L.) and Satureja thymbra L. populations from the Greek island Ikaria. Industrial Crops and Products, 33 (1): 236-241.
11. Milos, M., Mastelic, J., & Jerkovic, I. (2000). Chemical composition and antioxidant effect of glycosidically bound volatile compounds from oregano (Origanum vulgare L. ssp hirtum). Food Chemistry, 71 (1), 79-83.
12. Athanassiou, C.G., Hasan, M.M., Phillips, T.W., Aikins, M.J., & Throne, J.E. (2015). Efficacy of methyl bromide for control of different life stages of stored-product psocids. Journal of economic entomology, 108 (3), 1422-1428.
13. Abouseadaa, H.H., Osman, G.H., Ramadan, A.M., Hassanein, S.E., Abdelsattar, M.T., Morsy, Y.B., Alameldin, H.F., El-Ghareeb, D.K., Nour-Eldin, H.A., & Salem, R. (2015). Development of transgenic wheat (Triticum aestivum L.) expressing avidin gene conferring resistance to stored product insects. BMC plant biology, 15 (1): 183.
14. Rajashekar, Y., Ravindra, K., & Bakthavatsalam, N. (2014). Leaves of Lantana camara Linn. (Verbenaceae) as a potential insecticide for the management of three species of stored grain insect pests. Journal of food science and technology, 51 (11), 3494-3499.
15. Jagadeesan, R., Nayak, M.K., Pavic, H., Chandra, K., & Collins, P.J. (2015). Susceptibility to sulfuryl fluoride and lack of cross‐resistance to phosphine in developmental stages of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae). Pest management science, 71 (10), 1379-1386.
16. Isman, M.B. (2006). Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annu Rev Entomol, 51, 45-66.
17. Aref, S.P., Valizadegan, O., & Farashiani, M.E. (2015). Eucalyptus dundasii Maiden essential oil, chemical composition and insecticidal values against Rhyzopertha dominica (F.) and Oryzaephilus surinamensis (L.). Journal of Plant Protection Research, 55 (1), 35-41.
18. Mishra, B.B., Tripathi, S., & Tripathi, C. (2013). Bioactivity of two plant derived essential oils against the rice weevils Sitophilus oryzae (L.) (Coleoptera: Curculionidae). Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 83 (2), 171-175.
19. Shaaban, H.A.E., El-Ghorab, A.H., & Shibamoto, T. (2012). Bioactivity of essential oils and their volatile aroma components: Review. Journal of Essential Oil Research, 24 (2), 203-212.
20. Huang, Y., Lam, S., & Ho, S. (2000). Bioactivities of essential oil from Elletaria cardamomum (L.) Maton. to Sitophilus zeamais Motschulsky and Tribolium castaneum (Herbst). Journal of Stored Products Research, 36 (2), 107-117.
21. Abay, G., Karakoc, O.C., Tufekci, A.R., Koldas, S., & Demirtas, I. (2012). Insecticidal activity of Hypnum cupressiforme (Bryophyta) against Sitophilus granarius (Coleoptera: Curculionidae). Journal of Stored Products Research, 51, 6-10.
22. Polatoğlu, K., Karakoç, Ö.C., Yücel, Y.Y., Gücel, S., Demirci, B., Demirci, F., & Başer, K.H.C. (2017). Insecticidal activity of Salvia veneris Hedge. Essential oil against coleopteran stored product insects and Spodoptera exigua (Lepidoptera). Industrial Crops and Products, 97: 93-100.
23. LeOra Software (1994). Polo-PC a user’s guide to probit or logit analysis. 1119 Shattuck Avenue, Berkeley, CA, 94707: USA.
24. Schulz, H., Özkan, G., Baranska, M., Krüger, H., & Özcan, M. (2005). Characterisation of essential oil plants from Turkey by IR and Raman spectroscopy. Vibrational Spectroscopy, 39 (2), 249-256.
25. Khalfi, O., Sahraoui, N., Bentahar, F., & Boutekedjiret, C. (2008). Chemical composition and insecticidal properties of Origanum glandulosum (Desf.) essential oil from Algeria. Journal of the Science of Food and Agriculture, 88 (9), 1562-1566.
26. Souguir, S., Chaieb, I., Cheikh, Z.B., & Laarif, A. (2013). Insecticidal activities of essential oils from some cultivated aromatic plants against Spodoptera littoralis (Boisd). Journal of Plant Protection Research, 53 (4), 388-391.
27. Sahraoui, N., Hazzit, M., & Boutekedjiret, C. (2017). Effects of microwave heating on the antioxidant and insecticidal activities of essential oil of Origanum glandulosum Desf. obtained by microwave steam distillation. Journal of Essential Oil Research: 1-10.
28. Konstantopoulou, I., Vassilopoulou, L., Mavragani-Tsipidou, P., & Scouras, Z. (1992). Insecticidal effects of essential oils. A study of the effects of essential oils extracted from eleven Greek aromatic plants on Drosophila auraria. Experientia, 48 (6), 616-619.
29. Ahn, Y.-J., Lee, S.-B., Lee, H.-S., & Kim, G.-H. (1998). Insecticidal and acaricidal activity of carvacrol and β-thujaplicine derived from Thujopsis dolabrata var. hondai Sawdust. Journal of Chemical Ecology, 24 (1), 81-90.
30. Regnault-Roger, C. (1997). The potential of botanical essential oils for insect pest control. Integrated Pest Management Reviews, 2 (1), 25-34.
31. [31] El-Nahal, A., Schmidt, G., & Risha, E. (1989). Vapours of Acorus calamus oil—a space treatment for stored-product insects. Journal of Stored Products Research, 25 (4), 211-216.
32. Rani, P.U. (2012). Fumigant and contact toxic potential of essential oils from plant extracts against stored product pests. Journal of Biopesticides, 5 (2), 120.