Investigation of in vitro Enzyme Inhibitory Properties and Antioxidant Activity of Moltkia coerulea (Willd.) Lehm. (Boraginaceae) Growing in Raman Mountain - Batman

Abstract

The province of Batman is located in the Southeastern Anatolia Region of Turkey, and it is significant in terms of its ecosystem and plant biological diversity. Recently, researching economically important plant species has become a necessity in the province. In this context, while the members of the Boraginaceae genus have found a wide application area in traditional medicine in many countries from ancient times until today, they have been used for many purposes in Turkey. Most of the members of this family are medically important plants containing secondary metabolites such as flavonoids, terpenoids, alkaloids, fatty acids, glycosides, phytosterols, and various proteins. Moltkia coerulea (Willd.) Lehm. is found Anatolia, Lebanon and Crimea. This study aimed to determine the enzyme inhibition and antioxidant activity of M. coerulea (Willd.) Lehm, which has not been studied before, and grows in the untouched Raman Mountain in Batman. α-amylase and α-glucosidase inhibition results of methanolic (MeOH) and aqueous (Aq) extracts of M. coerulea were calculated as acarbose equivalents (ACAEs/g extract). Tyrosinase inhibition results of MeOH and Aq extracts of M. coerulea were calculated as kojic acid equivalent (mmol KAEs/g extract). Additionally, the extracts were tested against the 2,2-diphenyl-1-picrylhydrazyl (DPPH•) free-radical to analyze their antioxidant activity. The highest antioxidant activity was found in the leaf extract (MeOH) as 61.2 % with for the DPPH• method. These results showed that M. coerulea could be used as a potential source of natural antioxidant.

Keywords

• Antioxidant activity
• enzyme inhibition
• Moltkia Lehm.

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