Biological evaluation of Stachys iberica subsp. stenostachya (Boiss.) Rech.f. and Scutellaria orientalis subsp. sosnowskyi (Takht.) Fed. growing in eastern Anatolia

Abstract

Lamiaceae is one of the largest families in the plant kingdom, including the genus Stachys and Scutellaria, which are used in many folk medicines throughout the world for the prevention and also the treatment of several disorders. In vitro biological potential of Stachys iberica subsp. stenostachya and Scutellaria orientalis subsp. sosnowskyi were investigated in the current study. The aerial parts of the plants were extracted using different solvents such as n-hexane, chloroform, and methanol. In addition, infusions of each plant were prepared. The antioxidant potential of the samples was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ABTS assays, ferrous ion-chelating, and ferric reducing antioxidant power (FRAP) assays. Anticholinesterase activity of the extracts was also determined. Spectrophotometric analysis was used to assess the total phenolic content of the samples. The antimicrobial activities of samples were determined by minimal inhibitory concentration (MIC) against seven bacteria and three Candida spp. yeast. According to the findings, the infusion demonstrated significant antioxidant properties, whilst the extracts demonstrated high-to-moderate antioxidant effects. The n-hexane extracts showed higher antifungal activity against C.parapsilosis and C.tropicalis. These outcomes suggest that these two species from Turkey could be employed in the manufacture of phytopharmaceuticals.

Keywords

• Lamiaceae
• Turkey
• Antioxidant
• Antimicrobial
• Acetylcholinesterase

References

1. Açar, M, & Satıl, F. (2019). Distantes R. Bhattacharjee (Stachys L. /Lamiaceae) Altseksiyonu Taksonları Üzerinde Karşılaştırmalı Anatomik ve Mikromorfolojik Çalışmalar. KSÜ Tarım ve Doğa Derg, 22(Ek Sayı 2), 282-295. https://doi.org/10.18016/ksutarimdoga.vi.562089
2. Ahmad, V.U., Arshad, S., Bader, S., Iqbal, S., Khan, A., Khan, S.S., Hussain, J., Tareen, R.B., Ahmed, A. (2008). New terpenoids from Stachys parviflora Benth. Magn. Reson. Chem., 46(10), 986-989. https://doi.org/10.1002/mrc.2277
3. Akçiçek, E., Fırat, M., & Güner, Ö. (2016). Stachys hakkariensis (Lamiaceae), a new species from eastern Anatolia (Turkey) belonging to Stachys sect. Olisia. Phytotaxa, 257(2), 167–173. https://doi.org/10.11646/phytotaxa.257.2.6
4. Akkol, E.K., Orhan, I.E., Yeşilada, E. (2012). Anticholinesterase and antioxidant effects of the ethanol extract, ethanol fractions, and isolated flavonoids from Cistus laurifolius L. leaves. Food Chem., 131(2), 626-631. https://doi.org/10.1016/j.foodchem.2011.09.041
5. Apak, R., Güçlü, K., Özyürek, M., Karademir, S.E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J. Agric. Food Chem., 52(26), 7970-7981. https://doi.org/10.1021/jf048741x
6. Bahadori, M.B., Zengin, G., Dinparast, L., Eskandani, M. (2020). The health benefits of three Hedgenettle herbal teas (Stachys byzantina, Stachys inflata, and Stachys lavandulifolia)-profiling phenolic and antioxidant activities. Eur. J. Integr. Med., 36, 101134. https://doi.org/10.1016/j.eujim.2020.101134
7. Bardakci, H., Acar, E.T., Kırmızıbekmez, H. (2019). Simultaneous quantification of six flavonoids in four Scutellaria taxa by HPLC-DAD method. Rev. Bras. Farmacogn., 29, 17-23. https://doi.org/10.1016/j.bjp.2018.09.006
8. Baytop, T. (1999). Therapy with Medicinal Plants in Turkey, Past and Present. 2nd ed. Nobel Medical Publishing, Istanbul, pp. 142–144.

9. Benzie, I.F.F., Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal. Biochem., 239, 70 76. https://doi.org/10.1006/abio.1996.0292
10. Bibi, Sadeer, N., Montesano, D., Albrizio, S., Zengin, G., Mahomoodally, M.F. (2020). The versatility of antioxidant assays in food science and safety-Chemistry, applications, strengths, and limitations. Antioxidants., 9(8), 709. https://doi.org/10.3390/antiox9080709
11. Clinical and Laboratory Standards Institute (CLSI) (2020). Performance Standards for Antimicrobial Susceptibility Testing. Clinical and Laboratory Standards Institute, Wayne, PA.
12. Clinical and Laboratory Standards Institute (CLSI) (1997). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard–Second Edition. Clinical and Laboratory Standards Institute, Wayne, PA.
13. Çiçek, M. (2012). Scutellaria. In: Türkiye Bitkileri Listesi (Damarlı Bitkiler). Güner, A, Aslan, S., Ekim, T., Vural, M., & M.T., Babaç, Edits. Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, pp. 582-585.
14. Demirci, B., Göger, F., Özek, G., Duman, H., & Başer, K. H. C. (2016). Essential oil composition of Stachys iberica Bieb. subsp. iberica from Turkey. Natural Volatiles and Essential Oils, 3(1), 31-34.
15. Doğan, Z., Ishiuchi, K.I., Makino, T., Saraçoğlu, İ. (2019). Alpha-Pyrone Glycosides from Scutellaria Salviifolia Benth. Turk. J. Chem., 43(3), 972-981. https://doi.org/10.3906/kim-1811-58
16. Ebrahimabadi, A.H., Ebrahimabadi, E.H., Djafari-Bidgoli, Z., Kashi, F.J., Mazoochi, A., Batooli, H. (2010). Composition and antioxidant and antimicrobial activity of the essential oil and extracts of Stachys inflata Benth from Iran. Food Chem., 119(2), 452-458. https://doi.org/10.1016/j.foodchem.2009.06.037
17. Elfalleh, W., Kirkan, B., Sarikurkcu, C. (2019). Antioxidant potential and phenolic composition of extracts from Stachys tmolea: An endemic plant from Turkey. Ind. Crops Prod., 127, 212-216. https://doi.org/10.1016/j.indcrop.2018.10.078
18. Ellman, G.L., Courtney K.D., Andress V., Featherstone R.M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol., 7(2), 88-95. https://doi.org/10.1016/0006-2952(61)90145-9
19. Formisano, C., Rigano, D., Senatore, F., Piozzi, F., Arnold, N.A. (2011). Analysis of essential oils from Scutellaria orientalis ssp. alpina and S. utriculata by GC and GC-MS. Nat. Prod. Commun., 6(9), 1347-1350. https://doi.org/10.1177/1934578X1100600932
20. Frezza, C., Venditti, A., Serafini, M., Bianco, A. (2019). Phytochemistry, chemotaxonomy, ethnopharmacology, and nutraceutics of Lamiaceae. Stud. Nat. Prod. Chem., 62, 125-178. https://doi.org/10.1016/B978-0-444-64185-4.00004-6
21. Georgieva, Y., Katsarova, M., Stoyanov, P., Mladenov, R., Denev, P., Teneva, D., Plotnikov, E., Bozov, P., Dimitrova, S. (2021). Metabolite profile and antioxidant activity of some species of Genus Scutellaria growing in Bulgaria. Plants, 10(1), 45. https://doi.org/10.3390/plants10010045
22. Gibbons, S. (2005). Plants as a source of bacterial resistance modulators and anti-infective agents. Phytochem. Rev., 4(1), 63-78. https://doi.org/10.1007/s11101-005-2494-9
23. Güner, Ö., Akçiçek, E., & Dirmenci, T. 2021: A new Stachys species from Turkey: Stachys siirtensis (Lamiaceae). Phytotaxa, 516(3), 252–262.
24. İçen, M.S., Arabaci, T., Kostekci, S., Gürhan, İ. (2016). Chemical Composition of the Essential Oil of Scutellaria orientalis L. subsp. virens Boiss. & Kotschy JR Edm. from Turkey. Hacettepe J. Biol. Chem., 44(1), 25-28. https://doi.org/10.15671/HJBC.20164417563
25. Kartsev, V.G., Stepanichenko, N.N., Auelbekov, S.A. (1994). Chemical composition and pharmacological properties of plants of the genusStachys. Chem. Nat. Compd., 30(6), 645–654. https://doi.org/10.1007/BF00630595
26. Kaya, A., Demirci, B., Baser, K.H.C. (2001). The composition of the essential oil of Stachys iberica subsp. stenostachya growing in Turkey. Chem. Nat. Compd., 37(4), 326–328. https://doi.org/10.1023/A:1013762200024
27. Kocak, M.S., Uren, M.C., Calapoglu, M., Tepe, A.S., Mocan, A., Rengasamy, K.R.R., Sarikurkcu, C. (2017). Phenolic profile, antioxidant and enzyme inhibitory activities of Stachys annua subsp. annua var. annua. S. Afr. J. Bot., 113, 128 132. https://doi.org/10.1016/j.sajb.2017.08.005
28. Koşar, M., Göger, F., Can Başer, K.H. (2008). In vitro antioxidant properties and phenolic composition of Salvia virgata Jacq. from Turkey. J. Agric. Food Chem., 56(7), 2369–2374. https://doi.org/10.1021/jf073516b
29. Mamadalieva, N.Z., Sharopov, F., Satyal, P., Azimova, S.S., Wink, M. (2017). Composition of the essential oils of three Uzbek Scutellaria species (Lamiaceae) and their antioxidant activities. Nat. Prod. Res., 31(10), 1172 1176. https://doi.org/10.1080/14786419.2016.1222383
30. Minareci, E., Pekönür, S. (2017). An Important Euroasian Genus: Scutellaria L. Int. J. Sec. Metab., 4(1), 35–46. https://doi.org/10.21448/ijsm.282271
31. Nie, X.P., Qu, G.W., Yue, X.D., Li, G.S., Dai, S.J. (2010). Scutelinquanines A–C, three new cytotoxic neo-clerodane diterpenoid from Scutellaria barbata. Phytochem. Lett., 3(4), 190-193. https://doi.org/10.1016/j.phytol.2010.07.004
32. Orhan, D.D, Özçelik, B., Özgen, S., Ergun, F. (2010). Antibacterial, antifungal, and antiviral activities of some flavonoids. Microbiol. Res., 165(6), 496 504. https://doi.org/10.1016/j.micres.2009.09.002
33. Orhan, I.E., Üstün, O., Şener, B. (2011). Estimation of cholinesterase inhibitory and antioxidant effects of the leaf extracts of Anatolian Ficus carica var. domestica and their total phenol and flavonoid contents. Nat. Prod. Commun., 6(3), 375 378. https://doi.org/10.1177/1934578X1100600315
34. Ozsoy, N., Can, A., Yanardağ, R., Akev, N. (2008). Antioxidant activity of Smilax excelsa L. leaf extracts. Food Chem., 110(3), 571 583. https://doi.org/10.1016/j.foodchem.2008.02.037
35. Pieters, L., Vlietinck, A.J. (2005). Bioguided isolation of pharmacologically active plant components, still a valuable strategy for the finding of new lead compounds?. J. Ethnopharmacol., 100(1-2), 57-60. https://doi.org/10.1016/j.jep.2005.05.029
36. Saad, B., Azaizeh, H., Said, O. (2005). Tradition and perspectives of Arab herbal medicine: a review. Evid. Based Complementary Altern. Med., 2(4), 475 479. https://doi.org/10.1093/ecam/neh133
37. Satıl, F., & Açar, M. (2020). Ethnobotanical use of Stachys L. (Lamiaceae) taxa in Turkey. International Journal of Nature and Life Sciences, 4(2), 66-86.
38. Sato, Y., Suzaki, S., Nishikawa, T., Kihara, M., Shibata, H., Higuti, T. (2000). Phytochemical flavones isolated from Scutellaria barbata and antibacterial activity against methicillin-resistant Staphylococcus aureus. J. Ethnopharmacol., 72(3), 483 488. https://doi.org/10.1016/S0378-8741(00)00265-8
39. Sarikurkcu, C., Kocak, M.S., Uren, M.C., Calapoglu, M., Tepe, A.S. (2016). Potential sources for the management global health problems and oxidative stress: Stachys byzantina and S. iberica subsp. iberica var. densipilosa. Eur. J. Integr. Med., 8(5), 631 637. https://doi.org/10.1016/j.eujim.2016.04.010
40. Shahidi, F. (2000). Antioxidants in food and food antioxidants. Food/Nahrung, 44(3), 158–163. https://doi.org/10.1002/1521-3803(20000501)44:3<158:AID-FOOD158>3.0.CO;2-L
41. Shang, X., He, X., He, X., Li, M., Zhang, R., Fan, P., Zhang, Q., Jia, Z. (2010). The genus Scutellaria an ethnopharmacological and phytochemical review. J. Ethnopharmacol., 128(2), 279-313. https://doi.org/10.1016/j.jep.2010.01.006
42. Şenol, F.S., Orhan, I., Yilmaz, G., Cicek, M., Şener, B. (2010). Acetylcholinesterase, butyrylcholinesterase, and tyrosinase inhibition studies and antioxidant activities of 33 Scutellaria L. taxa from Turkey. Food Chem. Toxicol., 48(3), 781 788. https://doi.org/10.1016/j.fct.2009.12.004
43. Tao, G., Balunas, M.J. (2016). Current therapeutic role and medicinal potential of Scutellaria barbata in Traditional Chinese Medicine and Western research. J. Ethnopharmacol., 182, 170-180. https://doi.org/10.1016/j.jep.2016.02.012
44. Tomou, E.M., Barda, C., Skaltsa, H. (2020). Genus Stachys: A review of traditional uses, phytochemistry, and bioactivity. Medicines, 7(10), 63. https://doi.org/10.3390/medicines7100063
45. Tundis, R., Peruzzi, L., Menichini, F. (2014). Phytochemical and biological studies of Stachys species in relation to chemotaxonomy: a review. Phytochemistry, 102, 7 39. https://doi.org/10.1016/j.phytochem.2014.01.023
46. Vergun, O., Svydenko, L., Grygorieva, O., Shymanska, O., Rakhmetov, D., Brindza, J., Ivanišová, E. (2019). Antioxidant capacity of plant raw material of Scutellaria baicalensis Georgi. Potr., 13(1), 614-619. https://doi.org/10.5219/1090
47. Wei, F., Jinglou, C., Yaling, C., Yongfang, L., Liming, C., Lei, P. (2010). Antioxidant, free radical scavenging, anti-inflammatoryand hepatoprotective potential of the extract from Parathelypteris nipponica (Franch.etSav.) Ching. J. Ethnopharmacol., 130, 521–28. https://doi.org/10.1016/j.jep.2010.05.039
48. Yılmaz, G., Çiçek, M., Demirci, B., Başer, K.H.C. (2019). Essential oil compositions of subspecies of Scutellaria brevibracteata Stapf from Turkey. J. Essent. Oil Res., 31(4), 255–262. https://doi.org/10.1080/10412905.2019.1579762
49. Yilmaz, G., Şimşek, D., Altanlar, N., Çiçek, M. (2020). Comparison of the antimicrobial activity of some Scutellaria orientalis L. taxa growing in Turkey. Int. J. Second. Metab., 7(3), 192–199. https://doi.org/10.21448/ijsm.732568
50. Zengin, G., Llorent-Martínez, E.J., Molina-García, L., Fernández-de Córdova, M.L., Aktumsek, A., Uysal, S., Rengasamy K.R.R., Aumeedruddy, M.Z., Bahadori, M.B., Mahomoodally, M.F. (2019). Chemical profile, antioxidant, and enzyme inhibitory properties of two Scutellaria species: S. orientalis L. and S. salviifolia Benth. J. Pharm. Pharmacol., 71(2), 270–280. https://doi.org/10.1111/jphp.13030
51. Zhao, T., Tang, H., Xie, L., Zheng, Y., Ma, Z., Sun, Q., Li, X. (2019). Scutellaria baicalensis Georgi. (Lamiaceae): a review of its traditional uses, botany, phytochemistry, pharmacology and toxicology. J. Pharm. Pharmacol., 71(9), 1353 1369. https://doi.org/10.1111/jphp.13129