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Phenolic Contents and Antioxidant Properties of Echinops ritro L. and E. tournefortii Jaup. Et. Spach Extract

Yıl 2016, Cilt 3, Sayı 2, 74 - 81, 01.07.2016
https://doi.org/10.21448/http-ijate-net-index-php-ijsm.243309

Öz

Aim of the study was to evaluate antioxidant activity and total phenolic content of Echinops ritro L. and E. tournefortii (Asteraceae). The dried leaves and seeds of E. ritro and E. tournefortii were extracted separately with ethanol, methanol, chloroform and dH2O. Total phenolic content was measured by Folin-Ciocalteu method. Antioxidant activities of the extracts were determined by two test systems namely, radical scavenging on DPPH and β-carotene bleaching methods. dH2O extracts has the highest phenolic content (92.24 GAE mg/100g). The results were compared to those of BHT as synthetic antioxidant. dH2O extracts were found to be rich as a source of phenolics. According to the results of antioxidant activity, dH2O extracts exhibited higher antioxidant activity than all types of solvent. The strongest antioxidant properties were obtained by dH2O extract. Radical scavenging activities (%) were found to be in the following order: Chloroform<Ethanol<Methanol<dH2O<BHT. These results of the present study demonstrated that the extracts of E. ritro and E. tournefortii may be used as a source of natural antioxidant in the food and pharmaceutical industries.

Kaynakça

  • Lee, Y.M., Kim, H., Hong, E.K., Kang, BH., Kim, S.J. (2000). Water extract of 1:1 mixture of Phellodendron cortex and Aralia cortex has inhibitory effects on oxidative stress in kidney of diabetic rats. J. Ethnopharmacol., 73, 429-36.
  • Pai, S.R., Nimbalkar, M.S., Pawar, N.V., Patil, R.P., Dixit, GB. (2010). Seasonal discrepancy in phenolic content and antioxidant properties from bark of Nothopodytes nimmoniana (Grah.) Mabb. Int J Pharm Biosci., 1, 1-17.
  • Buyukokurouglu, ME., Gulcin, I., Oktay, M., Kufrevioglu, O.I. (2001). In vitro antioxidant properties of dantrolene sodium. Pharmacol Res. 44, 491.
  • Carvalho, R.N., Moura, L.S, Rosa, P.T.V, Meireles, M.A.A. (2005). Supercritical fluid extraction from rosemary (Rosmarinus officinalis): kinetic data, extract’s global yield,
  • composition, and antioxidant activity. J. Supercrit Fluids, 35, 197-204.
  • Susanna, A., Garcia-Jacas, N. The tribe Cardueae In: Kadereit J & Kubitzki K (eds.). Compositae. The Families and Genera of Vascular Plants. Heidelberg: Springer-Verlag, 2007, pp. 135-158.
  • Garnatje, T., Valles, J., Garcia, S., Hidalgo, O., Sanz, M., Canela, MA., Siljak-Yakovlev, S. (2004). Genome size in Echinops L. and related genera (Asteraceae, Cardueae): karyological, ecological and phylogenetic implications. Biol Cell. 96, 117-124.
  • Özhatay, N., Kültür, Ş., Aslan, S. (2009). Check-list of additional taxa to the Supplement Flora of Turkey IV. Turk J Bot. 33, 191-226.
  • Vural, C., Şapcı, H. (2012). Five new records of the genus Echinops (Asteraceae) from Turkey. Turk. J. Bot. 36: 151-160.
  • Shukla, Y.N. (2003). Chemical, botanical and pharmacological studies on the genus Echinops : A review. J. Med Arom Pl Sci., 25, 720-32.
  • Suarez, C., Barrera, C., Caballero, A. (2011). Quinolone alkaloids and friedelanetype triterpenes isolated from leaves and wood of Esenbeckia alata kunt Rutaceae. Quim. Nova, 34(6), 984-86.
  • Desta, B. (1993). Ethiopian Traditional Herbal Drugs Antimicrobial activity of 63 medicinal-plants. J. Ethnopharmacol., 39, 129-139.
  • Slinkard, K., Singleton, V.L. (1977). Total Phenol Analysis: Automation and Comparison with Manual Methods. American J. Enology and Viticulture., 28(1), 49-55. [13]. Amin, I., Tan, S.H. (2002). Antioxidant activity of selected commercial seaweeds. Malaysian Journal of Nutrition., 8, 167-177.
  • Wu, C., Chen, F., Wang, X., Kim, H., He, G., Haley-Zitlin, V., Huang, G. (2006). Antioxidant constituents in feverfew (Tanacetum parthenium) extract and their chromatographic quantification. Food Chem., 96, 220–227.
  • Yesıloglu, Y., Sıt, L., Kılıc, I. (2013). In vitro Antioxidant Activity and Total Phenolic Content of Various Extracts of Satureja hortensis L. Collected from Turkey. Asian Journal of Chemistry, 25(15), 8311-8316.
  • Khadri, A., Serralheiro, M.L.M., Nogueira, J.M.F., Neffati, M., Smiti, S., Araşjo, M.E.M. (2008). Antioxidant and antiacetylcholinesterase activities of essential oils from Cymbopogon schoenanthus L. Spreng. Determination of chemical composition by GC- mass spectrometry and 13C NMR. Food Chemistry, 109(3), 630–637.
  • Kamatou, G.P.P., Viljoen, A.M., Steenkamp, P. (2010). Antioxidant, anti-inflammatory activities and HPLC analysis of South African Salvia species. Food Chem., 119, 684- 688.
  • Krimat, S., Dob, T., Toumi, M., Kesouri, A., Noasri, A. (2015). Assessment of phytochemicals, antioxidant, antimicrobial and cytotoxic properties of Salvia chudaei Batt. et Trab. endemic medicinal plant from Algeria. J. Mater. Environ. Sci., 6(1), 70- 78.
  • Fokialakis, N., Cantrell, CL., Duke, SO., Skaltsounis, AL., Wedge, DE. Antifungal activity of thiophenes from Echinops ritro. J. Agric. Food Chem. 2006, 54(5), 1651-5.
  • Fokialakis, N., Kalpoutzakis, E., Tekwani, B.L., Khan, S.I., Kobaisy, M., Skaltsounis, A.L., Duke, S.O. (2007). Evaluation of the antimalarial and antileishmanial activity of plants from the Greek island of Crete. J. Nat. Med., 61, 38–45.
  • Erenler, R., Yilmaz, S., Aksit, H., Sen, O., Genc, N. Elmastas, M., Demirtas, I. (2014). Antioxidant Activities of Chemical Constituents Isolated from Echinops orientalis Trauv. Rec. Nat. Prod., 8(1), 32-36.
  • Afshaki, S., Jafari, A., Javidnia, K., Firuzi, O. (2012). Antioxidant and cytotoxic activities of four plant extracts from Dena region of Iran. Research in Pharmaceutical Sciences., 7(5), 853-9.
  • Rimbau, V., Cerdan, C., Vila, R., Iglesias, J. (1999). Antiinflammatory activity of Some extracts from plants used in the traditional medicine of North-African Countries. Phytother Res., 13(2), 128-32.

Phenolic Contents and Antioxidant Properties of Echinops ritro L. and E. tournefortii Jaup. Et. Spach Extract

Yıl 2016, Cilt 3, Sayı 2, 74 - 81, 01.07.2016
https://doi.org/10.21448/http-ijate-net-index-php-ijsm.243309

Öz

Aim of the study was to evaluate antioxidant activity and total phenolic content of Echinops ritro L. and E. tournefortii (Asteraceae). The dried leaves and seeds of E. ritro and E. tournefortii were extracted separately with ethanol, methanol, chloroform and dH2O. Total phenolic content was measured by Folin-Ciocalteu method. Antioxidant activities of the extracts were determined by two test systems namely, radical scavenging on DPPH and β-carotene bleaching methods. dH2O extracts has the highest phenolic content (92.24 GAE mg/100g). The results were compared to those of BHT as synthetic antioxidant. dH2O extracts were found to be rich as a source of phenolics. According to the results of antioxidant activity, dH2O extracts exhibited higher antioxidant activity than all types of solvent. The strongest antioxidant properties were obtained by dH2O extract. Radical scavenging activities (%) were found to be in the following order: Chloroform

Kaynakça

  • Lee, Y.M., Kim, H., Hong, E.K., Kang, BH., Kim, S.J. (2000). Water extract of 1:1 mixture of Phellodendron cortex and Aralia cortex has inhibitory effects on oxidative stress in kidney of diabetic rats. J. Ethnopharmacol., 73, 429-36.
  • Pai, S.R., Nimbalkar, M.S., Pawar, N.V., Patil, R.P., Dixit, GB. (2010). Seasonal discrepancy in phenolic content and antioxidant properties from bark of Nothopodytes nimmoniana (Grah.) Mabb. Int J Pharm Biosci., 1, 1-17.
  • Buyukokurouglu, ME., Gulcin, I., Oktay, M., Kufrevioglu, O.I. (2001). In vitro antioxidant properties of dantrolene sodium. Pharmacol Res. 44, 491.
  • Carvalho, R.N., Moura, L.S, Rosa, P.T.V, Meireles, M.A.A. (2005). Supercritical fluid extraction from rosemary (Rosmarinus officinalis): kinetic data, extract’s global yield,
  • composition, and antioxidant activity. J. Supercrit Fluids, 35, 197-204.
  • Susanna, A., Garcia-Jacas, N. The tribe Cardueae In: Kadereit J & Kubitzki K (eds.). Compositae. The Families and Genera of Vascular Plants. Heidelberg: Springer-Verlag, 2007, pp. 135-158.
  • Garnatje, T., Valles, J., Garcia, S., Hidalgo, O., Sanz, M., Canela, MA., Siljak-Yakovlev, S. (2004). Genome size in Echinops L. and related genera (Asteraceae, Cardueae): karyological, ecological and phylogenetic implications. Biol Cell. 96, 117-124.
  • Özhatay, N., Kültür, Ş., Aslan, S. (2009). Check-list of additional taxa to the Supplement Flora of Turkey IV. Turk J Bot. 33, 191-226.
  • Vural, C., Şapcı, H. (2012). Five new records of the genus Echinops (Asteraceae) from Turkey. Turk. J. Bot. 36: 151-160.
  • Shukla, Y.N. (2003). Chemical, botanical and pharmacological studies on the genus Echinops : A review. J. Med Arom Pl Sci., 25, 720-32.
  • Suarez, C., Barrera, C., Caballero, A. (2011). Quinolone alkaloids and friedelanetype triterpenes isolated from leaves and wood of Esenbeckia alata kunt Rutaceae. Quim. Nova, 34(6), 984-86.
  • Desta, B. (1993). Ethiopian Traditional Herbal Drugs Antimicrobial activity of 63 medicinal-plants. J. Ethnopharmacol., 39, 129-139.
  • Slinkard, K., Singleton, V.L. (1977). Total Phenol Analysis: Automation and Comparison with Manual Methods. American J. Enology and Viticulture., 28(1), 49-55. [13]. Amin, I., Tan, S.H. (2002). Antioxidant activity of selected commercial seaweeds. Malaysian Journal of Nutrition., 8, 167-177.
  • Wu, C., Chen, F., Wang, X., Kim, H., He, G., Haley-Zitlin, V., Huang, G. (2006). Antioxidant constituents in feverfew (Tanacetum parthenium) extract and their chromatographic quantification. Food Chem., 96, 220–227.
  • Yesıloglu, Y., Sıt, L., Kılıc, I. (2013). In vitro Antioxidant Activity and Total Phenolic Content of Various Extracts of Satureja hortensis L. Collected from Turkey. Asian Journal of Chemistry, 25(15), 8311-8316.
  • Khadri, A., Serralheiro, M.L.M., Nogueira, J.M.F., Neffati, M., Smiti, S., Araşjo, M.E.M. (2008). Antioxidant and antiacetylcholinesterase activities of essential oils from Cymbopogon schoenanthus L. Spreng. Determination of chemical composition by GC- mass spectrometry and 13C NMR. Food Chemistry, 109(3), 630–637.
  • Kamatou, G.P.P., Viljoen, A.M., Steenkamp, P. (2010). Antioxidant, anti-inflammatory activities and HPLC analysis of South African Salvia species. Food Chem., 119, 684- 688.
  • Krimat, S., Dob, T., Toumi, M., Kesouri, A., Noasri, A. (2015). Assessment of phytochemicals, antioxidant, antimicrobial and cytotoxic properties of Salvia chudaei Batt. et Trab. endemic medicinal plant from Algeria. J. Mater. Environ. Sci., 6(1), 70- 78.
  • Fokialakis, N., Cantrell, CL., Duke, SO., Skaltsounis, AL., Wedge, DE. Antifungal activity of thiophenes from Echinops ritro. J. Agric. Food Chem. 2006, 54(5), 1651-5.
  • Fokialakis, N., Kalpoutzakis, E., Tekwani, B.L., Khan, S.I., Kobaisy, M., Skaltsounis, A.L., Duke, S.O. (2007). Evaluation of the antimalarial and antileishmanial activity of plants from the Greek island of Crete. J. Nat. Med., 61, 38–45.
  • Erenler, R., Yilmaz, S., Aksit, H., Sen, O., Genc, N. Elmastas, M., Demirtas, I. (2014). Antioxidant Activities of Chemical Constituents Isolated from Echinops orientalis Trauv. Rec. Nat. Prod., 8(1), 32-36.
  • Afshaki, S., Jafari, A., Javidnia, K., Firuzi, O. (2012). Antioxidant and cytotoxic activities of four plant extracts from Dena region of Iran. Research in Pharmaceutical Sciences., 7(5), 853-9.
  • Rimbau, V., Cerdan, C., Vila, R., Iglesias, J. (1999). Antiinflammatory activity of Some extracts from plants used in the traditional medicine of North-African Countries. Phytother Res., 13(2), 128-32.

Ayrıntılar

Birincil Dil İngilizce
Konular Biyoloji
Yayınlanma Tarihi July
Bölüm Makaleler
Yazarlar

Çigdem AYDIN>

0000-0003-1934-9766


Gülten TAŞDELEN ÖZCAN Bu kişi benim


Murat TURAN>

0000-0003-2900-1755


Ramazan MAMMADOV>

Yayımlanma Tarihi 1 Temmuz 2016
Yayınlandığı Sayı Yıl 2016, Cilt 3, Sayı 2

Kaynak Göster

APA Aydın, Ç. , Taşdelen Özcan, G. , Turan, M. & Mammadov, R. (2016). Phenolic Contents and Antioxidant Properties of Echinops ritro L. and E. tournefortii Jaup. Et. Spach Extract . International Journal of Secondary Metabolite , 3 (2) , 74-81 . DOI: 10.21448/http-ijate-net-index-php-ijsm.243309
International Journal of Secondary Metabolite (IJSM)
ISSN-e: 2148-6905