Total Phenolics, Antioxidant, Antibacterial and Cytotoxic Activity Studies of Ethanolic Extracts Arisarum vulgare O.Targ.Tozz. and Dracunculus vulgaris Schott.
Yıl 2017,
Cilt: 4 Sayı: 2, 114 - 122, 01.07.2017
Çiğdem Aydın
,
Cennet Özay
,
Olcay Düşen
,
Ramazan Mammadov
,
Figen Orhan
Öz
The present study reports the antioxidant,
total phenolics, antibacterial and cytotoxic activities of Arisarum vulgare and Dracunculus vulgaris of aerial and under ground ethanol extracts. The
antioxidant activity was determined by DPPH radical scavenging activity and β-carotene
linoleic acid assays. Folin-Ciocalteu assay was used to detect total phenolic contents
in the extract. In vitro cytotoxic activity was determined by the Brine shrimp lethality test. Antibacterial
activity was investigated with the microdilution method. As a result, under ground parts of the extract showed higher
antioxidant activity than aerial parts of the extract. Total phenolics ranged from
14.5±2.02 to 53.4±2.01 mg GAE/g, and the antioxidant activity according
to the β-carotene/linoleic acid assay ranged from 70.76±1.08 % to 85.43±1.05 and according to the DPPH assay IC50 values ranged from 0.089±1.02 to 1.095±1.07mg/ml. The A. vulgare under ground extract was tested against 2 and showed a
good antibacterial activity at a concentration of <50 mg/mL Minimum inhibitory concentration (MIC) values for
the bacteria S.aureus. The results of
the cytotoxic
activities showed a very high activity of the extracts, D.vulgaris under ground extract (LC50 = 10.6 µg/mL).
Kaynakça
- Harman, D. (1992). Role of free radicals in aging and disease, Annals of the New York Academy of Sciences, 673, p. 126–141.
- Babu, V.L.A., and Gowri, R. (2010). Evaluation of antioxidant activity of Beta vulgaris root extract in rats, Asian Journal of Chemistry, 22(5): p.3385–3389.
- Romero, F.J., Bosch-Morell, F., Romero M.J., Jareño, E.J., Romero, B., Marín, N. and Romá, J. (1998). Lipid peroxidation products and antioxidants in human disease, Environmental Health Perspectives, 106(5): p.1229–1234.
- Janbaz, K.H., and Gilani A.H. (2000). Studies on preventive and curative effects of berberine on chemical-induced hepatotoxicity in rodents, Fitoterapia, 71(1): p. 25–33.
- Kumar, S., Chashoo, G., Saxena, A.K., and Pandey A.K. (2013). Parthenium hysterophorus: a probable source of anticancer, antioxidant and anti-HIV agent, BioMed Research International, 10: p. 734-745.
- Gramza-Michałowska, A., Abramowski, Z., Jovel Hęś, EM. (2008). Antioxidant potential of herbs extracts and impact on HEPG2 cells viability. Acta Sci. Pol. Technol. Aliment. 7(4): p.61-72.
- Jovanović, V.S., Simonović, S., Ilić, M., Marković, M., Mitić, V., Djordjevi A., and Mandic, SN. (2016). Chemical Composition, Antimicrobial and Antioxidant Activities of Seseli pallasii Besser. (syn Seseli varium Trev.) Essential Oils, Rec. Nat. Prod. 10(3): p.277-286.
- Kadri, H., Djilani, S.E., Djilani, A. (2013). Phytochemical Constituents, Antioxidant Activity, Total Phenolic and Flavonoid Contents of Arisarum vulgare Seeds. Acta Sci. Pol. Technol. Aliment. 12(2): p.169-173.
- Brussel, D.E. (2004). Medicinal plants of MT Pelion, Greece. Economic Botany, 58: p. 174-202.
- Baytop, T. (1999). Therapy with Medicinal Plants in Turkey (Past and Present), Second Edition, Nobel Presshouse, İstanbul, 361-362.
- Li, T.S.C. (2002). Medicinal Plants. Culture, Utilization and Phytopharmacology. CRC Press LLC. Boca Raton, Florida, USA. 8-50.
- Sağlık, S., Alpınar, K., Imre, S. (2002). Fatty acid composition of Dracunculus vulgaris Schott. (Araceae) seed oil from Turkey. Journal of Pharmaceutical Sciences, 5(3): p. 231-233.
- Mammadov, R., Ili, P., Ertem Vaizogullar, H. (2011). Antioxidant Activity and Total Phenolic Content of Gagea fibrosa and Romulea ramiflora. Iran. J. Chem. Chem. Eng. (IJCCE)., 30(3): p. 23-29.
- Slinkard, K., Singleton, V.L. (1977). Total phenol analyses: Automation and Comparison with Manual Methods. Am. J. Enol. Vitic., 28: p. 49-55.
- Amin, I., Norazaidah, Y., Hainida, KI. (2006). Antioxidant activity and phenolic content of raw and blanched Amaranthus species. Food Chem., 94: p.47–52.
- Wu, C., Chen, F., Wang, X., Kim, H., He, G., Haley-Zitlin, V., Huang, G. (2006). Antioxidant constituents in feverfew (Tanacetum parthenium) extract and their chromatographic quantification. Food Chem. 96: p. 220–227.
- Maclaughlın, J.L., Rogers, L., Anderson, J.E. (1994). The use of biological assays to evaluate botanicals. Drug Information Journal.,17: p.1-13.
- Satyajit, D., Sarker, L.N., Kumarasamy, Y., (2007). Microtitre Plate Based Antibacterial Assay Incorporating Resazurin As Indicator of Cell Growth, And Its Application in The in Vitro Antibacterial Screening of Phytochemicals. Methods., 42: p.321-324.
- Taherkhani, M. (2016). Chemical Investigation and Protective Effects of Bioactive Phytochemicals from Artemisia ciniformis, Iran. J. Chem. Chem. Eng. (IJCCE), 35(2): p.15-26.
- Zheng, W. and Wang, S.Y. (2001). Antioxidant activity and phenolic compounds in selected herbs. J. Agric. Food Chem., 49: p.5165-5170.
- Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Tech., 28: p. 25-30.
- Miura, K., Kikuzaki, H. and Nakatani, N. (2002). Antioxidant activity of chemical components from Sage (Salvia officinalis L.) and Thyme (Thymus vulgaris L) measured by the oil stability index method. J. Agric. Food Chem., 50: p.1845-1851.
- Exarchou, V., Nenadis, N., Tsimidou, M., Gerothanasis, I.P., Troganis, A. and Boskou, D. (2002). Antioxidant activities and phenolic composition of extracts from Greek oregano, Greek sage and summer savory. J. Agric. Food Chem., 50: p. 5294-5299.
- Esquivel, M.M., Ribeiro, M.A. and Bernardo-Gil, M.G. (1999). Supercritical extraction of Savory oil: Study of antioxidant activity and extract characterization. J. Supercrit. Fluid., 14: p.129-135.
- Öztürk, N. (2015). Phenolic composition and antioxidant activity of thedifferent extracts from Thymus longicaulis C Presl. subsp. longicaulis var. longicaulis and T. longicaulis C. Presl. subsp. longicaulis var. subisophyllus growing in Turkey. Pak. J. Pharm. Sci., 28(2): p.465-472.
- Aslantürk, Ö.S. and Çelik T.A. (2013). Potential AntioxidantActivity and Anticancer Effect of Extracts from Dracunculus vulgaris Schott. Tubers on MCF-7 Breast Cancer Cells, International Journal of Research in Pharmaceutical and Biomedical Sciences, 4(2): p.394-402.
- El-Desouky, S.K., Ryu, S.Y., and Kim, Y.K. (2007). Piperazirum, a novel bioactive alkaloid from Arum palaestinum Boiss. Tetrahedron Letters, 48: p. 4015-4017.
- Farid, M.M., Reda, S., Lamiaa, H., Ibrahim, F., El Desouky, M.A., Elsayed, A.M., El Oqlah, A.A., Saker, M.M. (2015). Cytotoxic activity and phytochemical analysis of Arum palaestinum Boiss. Asian Pac J Trop Biomed. 5(11): p.944–947.
Total Phenolics, Antioxidant, Antibacterial and Cytotoxic Activity Studies of Ethanolic Extracts Arisarum vulgare O.Targ.Tozz. and Dracunculus vulgaris Schott.
Yıl 2017,
Cilt: 4 Sayı: 2, 114 - 122, 01.07.2017
Çiğdem Aydın
,
Cennet Özay
,
Olcay Düşen
,
Ramazan Mammadov
,
Figen Orhan
Öz
The present study reports the antioxidant, total phenolics, antibacterial and cytotoxic activities of Arisarum vulgare and Dracunculus vulgaris of aerial and under ground ethanol extracts. The antioxidant activity was determined by DPPH radical scavenging activity and β-carotene linoleic acid assays. Folin-Ciocalteu assay was used to detect total phenolic contents in the extract. In vitro cytotoxic activity was determined by the Brine shrimp lethality test. Antibacterial activity was investigated with the microdilution method. As a result, under ground parts of the extract showed higher antioxidant activity than aerial parts of the extract. Total phenolics ranged from 14.5±2.02 to 53.4±2.01 mg GAE/g, and the antioxidant activity according to the β-carotene/linoleic acid assay ranged from 70.76±1.08 % to 85.43±1.05 and according to the DPPH assay IC50 values ranged from 0.089±1.02 to 1.095±1.07mg/ml. The A. vulgare under ground extract was tested against 2 and showed a good antibacterial activity at a concentration of <50 mg/mL Minimum inhibitory concentration (MIC) values for the bacteria S.aureus. The results of the cytotoxic activities showed a very high activity of the extracts, D.vulgaris under ground extract (LC50 = 10.6 µg/mL).
Kaynakça
- Harman, D. (1992). Role of free radicals in aging and disease, Annals of the New York Academy of Sciences, 673, p. 126–141.
- Babu, V.L.A., and Gowri, R. (2010). Evaluation of antioxidant activity of Beta vulgaris root extract in rats, Asian Journal of Chemistry, 22(5): p.3385–3389.
- Romero, F.J., Bosch-Morell, F., Romero M.J., Jareño, E.J., Romero, B., Marín, N. and Romá, J. (1998). Lipid peroxidation products and antioxidants in human disease, Environmental Health Perspectives, 106(5): p.1229–1234.
- Janbaz, K.H., and Gilani A.H. (2000). Studies on preventive and curative effects of berberine on chemical-induced hepatotoxicity in rodents, Fitoterapia, 71(1): p. 25–33.
- Kumar, S., Chashoo, G., Saxena, A.K., and Pandey A.K. (2013). Parthenium hysterophorus: a probable source of anticancer, antioxidant and anti-HIV agent, BioMed Research International, 10: p. 734-745.
- Gramza-Michałowska, A., Abramowski, Z., Jovel Hęś, EM. (2008). Antioxidant potential of herbs extracts and impact on HEPG2 cells viability. Acta Sci. Pol. Technol. Aliment. 7(4): p.61-72.
- Jovanović, V.S., Simonović, S., Ilić, M., Marković, M., Mitić, V., Djordjevi A., and Mandic, SN. (2016). Chemical Composition, Antimicrobial and Antioxidant Activities of Seseli pallasii Besser. (syn Seseli varium Trev.) Essential Oils, Rec. Nat. Prod. 10(3): p.277-286.
- Kadri, H., Djilani, S.E., Djilani, A. (2013). Phytochemical Constituents, Antioxidant Activity, Total Phenolic and Flavonoid Contents of Arisarum vulgare Seeds. Acta Sci. Pol. Technol. Aliment. 12(2): p.169-173.
- Brussel, D.E. (2004). Medicinal plants of MT Pelion, Greece. Economic Botany, 58: p. 174-202.
- Baytop, T. (1999). Therapy with Medicinal Plants in Turkey (Past and Present), Second Edition, Nobel Presshouse, İstanbul, 361-362.
- Li, T.S.C. (2002). Medicinal Plants. Culture, Utilization and Phytopharmacology. CRC Press LLC. Boca Raton, Florida, USA. 8-50.
- Sağlık, S., Alpınar, K., Imre, S. (2002). Fatty acid composition of Dracunculus vulgaris Schott. (Araceae) seed oil from Turkey. Journal of Pharmaceutical Sciences, 5(3): p. 231-233.
- Mammadov, R., Ili, P., Ertem Vaizogullar, H. (2011). Antioxidant Activity and Total Phenolic Content of Gagea fibrosa and Romulea ramiflora. Iran. J. Chem. Chem. Eng. (IJCCE)., 30(3): p. 23-29.
- Slinkard, K., Singleton, V.L. (1977). Total phenol analyses: Automation and Comparison with Manual Methods. Am. J. Enol. Vitic., 28: p. 49-55.
- Amin, I., Norazaidah, Y., Hainida, KI. (2006). Antioxidant activity and phenolic content of raw and blanched Amaranthus species. Food Chem., 94: p.47–52.
- Wu, C., Chen, F., Wang, X., Kim, H., He, G., Haley-Zitlin, V., Huang, G. (2006). Antioxidant constituents in feverfew (Tanacetum parthenium) extract and their chromatographic quantification. Food Chem. 96: p. 220–227.
- Maclaughlın, J.L., Rogers, L., Anderson, J.E. (1994). The use of biological assays to evaluate botanicals. Drug Information Journal.,17: p.1-13.
- Satyajit, D., Sarker, L.N., Kumarasamy, Y., (2007). Microtitre Plate Based Antibacterial Assay Incorporating Resazurin As Indicator of Cell Growth, And Its Application in The in Vitro Antibacterial Screening of Phytochemicals. Methods., 42: p.321-324.
- Taherkhani, M. (2016). Chemical Investigation and Protective Effects of Bioactive Phytochemicals from Artemisia ciniformis, Iran. J. Chem. Chem. Eng. (IJCCE), 35(2): p.15-26.
- Zheng, W. and Wang, S.Y. (2001). Antioxidant activity and phenolic compounds in selected herbs. J. Agric. Food Chem., 49: p.5165-5170.
- Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Tech., 28: p. 25-30.
- Miura, K., Kikuzaki, H. and Nakatani, N. (2002). Antioxidant activity of chemical components from Sage (Salvia officinalis L.) and Thyme (Thymus vulgaris L) measured by the oil stability index method. J. Agric. Food Chem., 50: p.1845-1851.
- Exarchou, V., Nenadis, N., Tsimidou, M., Gerothanasis, I.P., Troganis, A. and Boskou, D. (2002). Antioxidant activities and phenolic composition of extracts from Greek oregano, Greek sage and summer savory. J. Agric. Food Chem., 50: p. 5294-5299.
- Esquivel, M.M., Ribeiro, M.A. and Bernardo-Gil, M.G. (1999). Supercritical extraction of Savory oil: Study of antioxidant activity and extract characterization. J. Supercrit. Fluid., 14: p.129-135.
- Öztürk, N. (2015). Phenolic composition and antioxidant activity of thedifferent extracts from Thymus longicaulis C Presl. subsp. longicaulis var. longicaulis and T. longicaulis C. Presl. subsp. longicaulis var. subisophyllus growing in Turkey. Pak. J. Pharm. Sci., 28(2): p.465-472.
- Aslantürk, Ö.S. and Çelik T.A. (2013). Potential AntioxidantActivity and Anticancer Effect of Extracts from Dracunculus vulgaris Schott. Tubers on MCF-7 Breast Cancer Cells, International Journal of Research in Pharmaceutical and Biomedical Sciences, 4(2): p.394-402.
- El-Desouky, S.K., Ryu, S.Y., and Kim, Y.K. (2007). Piperazirum, a novel bioactive alkaloid from Arum palaestinum Boiss. Tetrahedron Letters, 48: p. 4015-4017.
- Farid, M.M., Reda, S., Lamiaa, H., Ibrahim, F., El Desouky, M.A., Elsayed, A.M., El Oqlah, A.A., Saker, M.M. (2015). Cytotoxic activity and phytochemical analysis of Arum palaestinum Boiss. Asian Pac J Trop Biomed. 5(11): p.944–947.