Molecular identification and phytochemical profiling of kamiling (wild toxic plant) using thin layer chromatography

Year 2018, Volume: 5 Issue: 3, 217 - 223, 15.09.2018

Dana Theresa C. De Leon John Dave C. Aquino Mary Jhane G. Valentino Jerwin R. Undan

https://doi.org/10.21448/ijsm.453009

Abstract

Some
plants may only be known by the people in their own respective place.
Consequently, there is a need for the authentication and further study of wild
toxic plant known by indigenous people. The genomic DNA from the leaves of the
plant Kamiling collected in Imugan, Santa Fe, Nueva Vizcaya, Philippines was
extracted and amplified using matK
and rbcL gene markers. Sample sequences was queried on
GenBank using Basic Local Alignment Search Tool (BLAST) revealed that the
sample sequences were identified as Semecarpus
cuneiformis using both on matK and
rbcL markers. The leaves of Kamiling was subjected through
Thin Layer Chromatography (TLC) and it revealed important phytochemicals such
as alkaloids, coumarins,
anthraquinones, anthrones, tannins, flavonoids, higher alcohols, phenols,
steroids and essential oils. Since there
is a growing awareness in correlating the phytochemical constituents of plants
with their pharmacological activity, wild toxic plant can be one of the
preferences for medicinal uses.

Keywords

TLC, matK, rbcL, wild toxic plant

Molecular identification and phytochemical profiling of kamiling (wild toxic plant) using thin layer chromatography

Abstract

Some plants may only be known by the people in their own respective place. Consequently, there is a need for the authentication and further study of wild toxic plant known by indigenous people. The genomic DNA from the leaves of the plant Kamiling collected in Imugan, Santa Fe, Nueva Vizcaya, Philippines was extracted and amplified using matK and rbcL gene markers. Sample sequences was queried on GenBank using Basic Local Alignment Search Tool (BLAST) revealed that the sample sequences were identified as Semecarpus cuneiformis using both on matK and rbcL markers. The leaves of Kamiling was subjected through Thin Layer Chromatography (TLC) and it revealed important phytochemicals such as alkaloids, coumarins, anthraquinones, anthrones, tannins, flavonoids, higher alcohols, phenols, steroids and essential oils. Since there is a growing awareness in correlating the phytochemical constituents of plants with their pharmacological activity, wild toxic plant can be one of the preferences for medicinal uses.

Keywords

TLC, matK, rbcL, wild toxic plant

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