Evaluation of Antioxidant, Antimicrobial and Antimutagenic Activity with Irritation Effects of Ceramium rubrum (Red Algae) Extract

Year 2018, Volume: 5 Issue: 4, 279 - 287, 29.12.2018

Adem Güner , Nefise Ülkü Karabay Yavaşoğlu

https://doi.org/10.21448/ijsm.432654

Cited By: 9

Abstract

This
study was conducted to evaluate antioxidant, antimutagenic and antimicrobial
activities as well as irritation/toxicity efficacy of n-hexane, chloroform and
methanol extracts of the red algae Ceramium
rubrum C. Agardh (1881). While spectrophotometric methods were used for the
determination of total phenol and flavonoid content, antioxidant activity was
evaluated by DPPH assay. Antimicrobial and antimutagenic efficacy of extracts
were determined by MIC method and Ames test, respectively. Also, HET-CAM test
was used for irritation/toxicity of extracts. While methanol extract was found
to have higher total flavonoid and phenolic contents than the other extracts
and hexane showed higher antioxidant activity than other extracts. All extracts
exerted moderate antimicrobial activity against tested microorganisms as 64–256
μg/mL. However, extracts of the algae did not show any mutagenicity or
irritation effect even at the highest concentration.

Keywords

Red algae, antioxidant, antimicrobial, Ames test, Hetcam assay

Evaluation of Antioxidant, Antimicrobial and Antimutagenic Activity with Irritation Effects of Ceramium rubrum (Red Algae) Extract

Abstract

This study was conducted to evaluate antioxidant, antimutagenic and antimicrobial activities as well as irritation/toxicity efficacy of n-hexane, chloroform and methanol extracts of the red algae Ceramium rubrum C. Agardh (1881). While spectrophotometric methods were used for the determination of total phenol and flavonoid content, antioxidant activity was evaluated by DPPH assay. Antimicrobial and antimutagenic efficacy of extracts were determined by MIC method and Ames test, respectively. Also, HET-CAM test was used for irritation/toxicity of extracts. While methanol extract was found to have higher total flavonoid and phenolic contents than the other extracts and hexane showed higher antioxidant activity than other extracts. All extracts exerted moderate antimicrobial activity against tested microorganisms as 64–256 μg/mL. However, extracts of the algae did not show any mutagenicity or irritation effect even at the highest concentration.

Keywords

Red algae, Antioxidant, Antimicrobial, Ames test, Hetcam assay

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