Biological activity studies on Klasea serratuloides (DC) Greuter & Wagenitz subsp. karamanica B. Dogan & A. Duran extracts obtained with different extraction methods

Yıl 2021, Cilt: 8 Sayı: 4, 389 - 397, 26.12.2021

Nuraniye Eruygur Fatma Ayaz Yavuz Bağcı Süleyman Doğu Tuğsen Doğru Merve Koçak

https://doi.org/10.21448/ijsm.942102

Cited By: 1

Öz

The aim of this study was to investigate the antioxidant and enzyme inhibition activities of methanol extracts prepared from Klasea serratuloides (DC) Greuter & Wagenitz subsp. karamanica B. Dogan & A. Duran with Soxhlet, ultrasonic extraction, and maceration methods. 1,1-Diphenyl-2-picryl hydroxyl (DPPH) quenching assay and 2,2’-azinobis-3- ethylbenzothiozoline-6-sulfonic acid (ABTS) cation decolorization test were used to evaluate in vitro radical scavenging activity. The total phenolic content was determined with the Folin-Ciocalteu method while the total flavonoid content was evaluated by the aluminum chloride colorimetric method. According to the results, DPPH, ABTS radical scavenging activity and iron chelating activity of methanol of Klasea serratuloides were shown concentration-dependent manner. The extract obtained from maceration was found to be higher than the other extracts. It suggests that the maceration technique was more effective than the other extraction methods for the determination of the phenolic content. The methanol extract of KS using soxhlet (61.17 ± 3.62) and ultrasonic extraction (58.76 ± 1.46) showed higher inhibition than the extract prepared with maceration methods (34.54 ± 0.73) against BChE. All extracts displayed moderate inhibition activity against AChE. As for enzyme inhibition activity, the extract from soxhlet method was found to be more potent tyrosinase, acetylcholinesterase and butyrylcholinesterase inhibitors than the other extracts prepared by ultrasound assisted extraction and maceration methods. The present study suggests that K. serratuloides should be given special attention to conduct further investigation for its phytochemical constituents that attribute to their antioxidant potentials, and enzyme inhibition activities. 

Anahtar Kelimeler

Klasea serratuloides, Antioxidant, Acetylcholinesterase, Butyrylcholinesterase, Tyrosinase

Destekleyen Kurum

Selcuk University Scientific Research Unit

Proje Numarası

University Research Grant SUBAP-18401141

Teşekkür

We acknowledge the Selcuk University Scientific Research Unit for the partial financial support (University Research Grant No: SUBAP-18401141) for this study.

Kaynakça

• Annales, S., Fennici, B., Ranjbar, M., Negaresh, K., Karamian, R. (2012). Klasea nana (Asteraceae), a new species from NE Iran. Annales Botanici Fennici, 49(5), 402-406.
• Atanassova, M., Georgieva, S., Ivancheva, K. (2011). Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs. Journal of the University of Chemical Technology and Metallurgy, 46, 81-88.
• Babacan, E.Y., Vitek, E., Çakılcıoğlu, U. (2017). Contributions to the Flora of Tunceli(Turkey). International Journal of Nature and Life Science, 1(2), 39-66.
• Baydoun, S., Chalak, L., Dalleh, H., Arnold, N. (2015). Ethnopharmacological survey of medicinal plants used in traditional medicine by the communities of Mount Hermon , Lebanon. Journal of Ethnopharmacology, 173, 139 156. https://doi.org/10.1016/j.jep.2015.06.052
• Chai, T., Mohan, M., Ong, H. ve Wong, F. (2014) Antioxidant, iron-chelating and anti-glucosidase activities of Typha domingensis Pers (Typhaceae), Tropical Journal of Pharmaceutical Research, 13(1), 67-72.
• Dogan, B., Duran, A., Coşkun, F. (2014). Klasea serratuloides subsp. karamanica subsp. nov. (Asteraceae), from the central and south Anatolia transition zone, Turkey. Nordic Journal of Botany, 32(4), 479-484. https://doi.org/10.1111/j.1756-1051.2013.00375.x
• Ellman, G. L., Courtney, K. D., Andres Jr, V., Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7(2), 88-95.
• Eruygur, N., Ucar, E., Aşkın, H., Keyhan, A., Seyed, S., Safavi, M. (2019). In vitro antioxidant assessment , screening of enzyme inhibitory activities of MeOH and water extracts and gene expression in Hypericum lydium. Molecular Biology Reports, 46(2), 2121-2129. https://doi.org/10.1007/s11033-019-04664-3
• Eruygur, N., Ucar, E., Ataş, M., Ergul, M., Ergul, M., Sozmen, F. (2020). Determination of biological activity of Tragopogon porrifolius and Polygonum cognatum consumed intensively by people in Sivas. Toxicology Reports, 7, 59 66. https://doi.org/10.1016/j.toxrep.2019.12.002
• Gan, R.Y., Xu, X.R., Song, F.L., Kuang, L., Li, H. (2010). Antioxidant activity and total phenolic content of medicinal plants associated with prevention and treatment of cardiovascular and cerebrovascular diseases. Journal of Medicinal Plants Research, 4(22), 2438-2444. https://doi.org/10.5897/JMPR10.581
• Likhitwitayawuid, K. (2008). Stilbenes with tyrosinase inhibitory activity. Current Science, 94(1), 44-52.
• Liyanaarachchi, G.D., Samarasekera, J.K.R.R., Mahanama, K.R.R., Hemalal, K.D.P. (2018). Tyrosinase, elastase, hyaluronidase, inhibitory and antioxidant activity of Sri Lankan medicinal plants for novel cosmeceuticals. Industrial Crops and Products, 111, 597-605. https://doi.org/10.1016/j.indcrop.2017.11.019
• Mao, P., Manczak, M., Shirendeb, U.P., Reddy, P.H. (2013). MitoQ, a mitochondria-targeted antioxidant, delays disease progression and alleviates pathogenesis in an experimental autoimmune encephalomyelitis mouse model of multiple sclerosis. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1832(12), 2322 2331. https://doi.org/10.1016/j.bbadis.2013.09.005
• Orhan, G., Orhan, I., Öztekin-Subutay, N., Ak, F., & Şener, B. (2009). Contemporary anticholinesterase pharmaceuticals of natural origin and their synthetic analogues for the treatment of Alzheimer’s disease. Recent Patents on CNS Drug Discovery, 4, 43-51. https://doi.org/10.2174/157488909787002582
• Rice-Evans, C.A., Miller, N.J., & Paganga, G. (1997). Antioxidant properties of phenolic compounds. Trends in Plant Science, 2, 152–159. https://doi.org/10.1016/S1360-1385(97)01018-2
• Senol, F.S., Orhan, I.E., Kurkcuoglu, M., Khan, M.T.H., Altintas, A., Sener, B., & Baser, K.H.C. (2013). A mechanistic investigation on anticholinesterase and antioxidant effects of rose (Rosa damascena Mill.). Food Research International, 53(1), 502 509. https://doi.org/10.1016/j.foodres.2013.05.031
• Szymanska, R., Pospíšil, P., Kruk, J. (2018). Plant-derived antioxidants in disease prevention 2018. Oxidative Medicine and Cellular Longevity, 2018, 2 4. https://doi.org/10.1155/2018/2068370
• Tel, G., Doğan, B., Erol, E., Öztürk, M., Nadeem, S., Ullah, Z., Duru, M.E., & Duran, A. (2015). Determination of antioxidant, anticholinesterase, tyrosinase inhibitory activities and fatty acid profiles of 10 anatolian Klasea cass. species. Records of Natural Products, 10, 122-127.
• Yang, Z., Wang, Y., Wang, Y., & Zhang, Y. (2012) Bioassay-guided screening and isolation of α-glucosidase and tyrosinase inhibitors from leaves of Morus alba. Food chemistry, 131(2), 617-625.