Comparison of three different protocols of alkaloid extraction from Glaucium corniculatum plant

Yıl 2022, Cilt: 9 Sayı: 1, 43 - 51, 10.03.2022

Fatma Gonca Koçancı Serap Niğdelioğlu Dolanbay Belma Aslım

https://doi.org/10.21448/ijsm.980171

Öz

Alkaloids, plant secondary metabolites, have a wide variety of biological effects. For this reason, the extraction of alkaloids from plants is of strategic importance. Different extraction protocols for the extraction of alkaloids from plants have been described by many authors. The objective of this study was to compare the efficacy of three different protocols for the extraction of alkaloids from Glaucium corniculatum. This article compares the Soxhlet and ultrasonication protocol, used in previous studies, to a modified Soxhlet protocol. While the alkaloid amount in the extract was determined by the spectrophotometric method, the qualitative estimation of the compounds in the extract was determined by Gas chromatograph-Mass spectrometer (GC-MS). The alkaloid amount and diversity in the extract, obtained through the recommended modified Soxhlet protocol, were higher than that of any extract obtained through other protocols. Thus, a new modified alkaloid extraction protocol was established, which is better than the other protocol.

Anahtar Kelimeler

Alkaloid, Extraction, Glaucium corniculatum, Ultrasonication, Soxhlet

Destekleyen Kurum

Tübitak

Proje Numarası

116S299

Kaynakça

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