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Phytochemical screening and in vitro antioxidant activities of Mentha suaveolens Ehrh. extract

Year 2023, Volume: 10 Issue: 3, 332 - 344, 27.08.2023
https://doi.org/10.21448/ijsm.1148664

Abstract

Within the framework of the valorization of the medicinal and aromatic plants of Morocco, we were interested during this study in the characterization and the phytochemical identification of some secondary metabolites present in Mentha suaveolens Ehrh. and the evaluation of the antioxidant activity of this species using four methods: DPPH free radical scavenging test, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid, (ABTS) radical cation scavenging test, FRAP test measuring antioxidant power, and β bleaching-carotene. Mentha suaveolens is a species belonging to the Lamiaceae family, harvested in southern Morocco, and widely used in traditional medicine for its biological properties attributed mainly to phenolic compounds. Just as they protect plants, secondary metabolites also have a protective role in the human body and are therefore beneficial to our health. They are attributed, in particular, with antioxidant, anti-inflammatory, and antibacterial properties. In this regard, characterization and identification tests of secondary metabolites revealed the presence of alkaloids, flavonoids, catechic tannins, and terpenes in this plant, whereas, the aerial parts of this species are devoid of coumarins, cyanogenic compounds, saponins and free quinones. The quantification of the phenolic compounds gave high contents, with contents of total phenols (54.75 ± 5.62) μg GAE/mg, total flavonoids (32.41 ± 0.41) μg QE/mg, and total condensed tannins (27 ± 1) μg CE/mg. In addition, the results obtained show that Mentha suaveolens extract has stronger antioxidant activity using the β-Carotene method with IC50 (0.24 ± 0.06) mg/mL against IC50 (0.021 ± 0.001) mg/mL for the standard antioxidant by the DPPH free radical scavenging test.

References

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  • Bhat, M.A., Al-Omar, M.A., & Siddiqui, N. (2013). Antimicrobial activity of Schiff bases of coumarin-incorporated 1, 3, 4-oxadiazole derivatives: an in vitro evaluation. Medicinal Chemistry Research, 22(9), 4455-4458. https://doi.org/10.1007/s00044-012-0452-9
  • Bichra, M., & Benkhalti, F. (2012). Antioxidant and anti-browning activities of Mentha suaveolens extracts. African Journal of Biotechnology, 11(35), 8722-8729.
  • Bichra, M., El-Modafar, C., El-Abbassi, A., Bouamama, H., & Benkhalti, F. (2013). Antioxidant activities and phenolic profile of six Moroccan selected herbs. Journal of Microbiology, Biotechnology and Food Sciences, 2(4), 2320 2338. https://office2.jmbfs.org/index.php/JMBFS/article/view/7495
  • Bimakr, M., Rahman, R.A., Taip, F. S., Ganjloo, A., Salleh, L.M., Selamat, J., . . . Zaidul, I. (2011). Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food and bioproducts processing, 89(1), 67-72. https://doi.org/10.1016/j.fbp.2010.03.002
  • Bougatef, A., Hajji, M., Balti, R., Lassoued, I., Triki-Ellouz, Y., & Nasri, M. (2009). Antioxidant and free radical-scavenging activities of smooth hound (Mustelus mustelus) muscle protein hydrolysates obtained by gastrointestinal proteases. Food Chemistry, 114(4), 1198-1205. https://doi.org/10.1016/j.foodchem.2008.10.075
  • Božović, M., Pirolli, A., & Ragno, R. (2015). Mentha suaveolens Ehrh.(Lamiaceae) essential oil and its main constituent piperitenone oxide: Biological activities and chemistry. Molecules, 20(5), 8605-8633. https://doi.org/10.3390/molecules20058605
  • Bruneton, J. (2009). Pharmacognosie, phytochimie et plantes médicinales [Pharmacognosy, phytochemistry and medicinal plants]. 4e éd, revue et augmentée, Paris, Tec & Doc, Éditions médicales internationales, 1288p.
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  • Dohou, R., Yamni, K., Tahrouch, S., Hassani, L.I., Badoc, A., & Gmira, N. (2003). Screening phytochimique d'une endémique iberomarocaine, Thymelaea lythroides [Phytochemical screening of an endemic iberomarocaine, Thymelaea lythroides]. Bulletin-Société de Pharmacie de Bordeaux, 142(1/4), 61-78.
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  • Hiermann, A., Schramm, H., & Laufer, S. (1998). Anti-inflammatory activity of myricetin-3-O-β-D-glucuronide and related compounds. Inflammation Research, 47(11), 421-427. https://doi.org/10.1007/s000110050355
  • Hmamouchi, M. (1999). Les plantes médicinales et aromatiques marocaines: utilisation, biologie, écologie, chimie, pharmacologie, toxicologie, lexiques.[ Moroccan medicinal and aromatic plants: use, biology, ecology, chemistry, pharmacology, toxicology, glossaries.] Bibliographie du patrimoine culturel immatériel, 1999.
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  • Jamila, F., & Mostafa, E. (2014). Ethnobotanical survey of medicinal plants used by people in Oriental Morocco to manage various ailments. Journal of Ethnopharmacology, 154(1), 76-87. https://doi.org/10.1016/j.jep.2014.03.016
  • Kabran G.R., N.C.A., J.A. Mamyrbékova-Békro, and Y.A. Békro. (2011). CCM d’extraits sélectifs de 10 plantes utilisées dans le traitement Traditionnel du Cancer du sein en Côte d’Ivoire. [TLC of selective extracts of 10 plants used in the traditional treatment of breast cancer in Côte d'Ivoire.]. Europ.J.Scientific.Research, 63(4), 592-603.
  • Kandaswamy, N., & Raveendiran, N. (2014). Synthesis, Characterization and Antifungal activity of Coumarin based random copolyester. American Journal of Research, 4(3), 704-707.
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  • Kasrati, A., Alaoui Jamali, C., & Abbad, A. (2017). Antioxidant properties of various extract from selected wild Moroccan aromatic and medicinal species. Trends in Phytochemical Research, 1(4), 175-182. https://dorl.net/dor/20.1001.1.25883623.2017.1.4.1.8
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  • Kumar, P., Mishra, S., Malik, A., & Satya, S. (2011). Insecticidal properties of Mentha species: a review. Industrial Crops and Products, 34(1), 802-817.
  • Loo, A., Jain, K., & Darah, I. (2008). Antioxidant activity of compounds isolated from the pyroligneous acid, Rhizophora apiculata. Food chemistry, 107(3), 1151-1160. https://doi.org/10.1016/j.foodchem.2007.09.044
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Phytochemical screening and in vitro antioxidant activities of Mentha suaveolens Ehrh. extract

Year 2023, Volume: 10 Issue: 3, 332 - 344, 27.08.2023
https://doi.org/10.21448/ijsm.1148664

Abstract

Within the framework of the valorization of the medicinal and aromatic plants of Morocco, we were interested during this study in the characterization and the phytochemical identification of some secondary metabolites present in Mentha suaveolens Ehrh. and the evaluation of the antioxidant activity of this species using four methods: DPPH free radical scavenging test, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid, (ABTS) radical cation scavenging test, FRAP test measuring antioxidant power, and β bleaching-carotene. Mentha suaveolens is a species belonging to the Lamiaceae family, harvested in southern Morocco, and widely used in traditional medicine for its biological properties attributed mainly to phenolic compounds. Just as they protect plants, secondary metabolites also have a protective role in the human body and are therefore beneficial to our health. They are attributed, in particular, with antioxidant, anti-inflammatory, and antibacterial properties. In this regard, characterization and identification tests of secondary metabolites revealed the presence of alkaloids, flavonoids, catechic tannins, and terpenes in this plant, whereas, the aerial parts of this species are devoid of coumarins, cyanogenic compounds, saponins and free quinones. The quantification of the phenolic compounds gave high contents, with contents of total phenols (54.75 ± 5.62) μg GAE/mg, total flavonoids (32.41 ± 0.41) μg QE/mg, and total condensed tannins (27 ± 1) μg CE/mg. In addition, the results obtained show that Mentha suaveolens extract has stronger antioxidant activity using the β-Carotene method with IC50 (0.24 ± 0.06) mg/mL against IC50 (0.021 ± 0.001) mg/mL for the standard antioxidant by the DPPH free radical scavenging test.

References

  • Afrokh, M., Tahrouch, S., ELMehrach, K., Fahmi, F., Bihi, M.A., Weber-Ravn, H., . . . Tabyaoui, M. (2023). Ethnobotanical, phytochemical and antioxidant study of fifty aromatic and medicinal plants. Chemical Data Collections, 43, 100984. https://doi.org/10.1016/j.cdc.2022.100984
  • Alilou, H., Bencharki, B., Hassani, L.I., & Barka, N. (2014). Screening phytochimique et identification spectroscopique des flavonoïdes d’Asteriscus graveolens subsp. odorus.[ Phytochemical screening and spectroscopic identification of flavonoids from Asteriscus graveolens subsp. odorus]. Afrique Science: Revue Internationale des Sciences et Technologie, 10(3).
  • Bellakhdar, J. (1996). Medicinal plants in North Africa and basic care in. Hand book of Modern Herbal Medicine. Le Fennec. Medicinal plants in North Africa and basic care in. Hand book of Modern Herbal Medicine. Le Fennec, 385.
  • Bellakhdar, J. (2006). Plantes médicinales au Maghreb et soins de base [Maghreb medicinal plants and basic care]: précis de phytothérapie moderne (Le Fennec ed.).
  • Bhat, M.A., Al-Omar, M.A., & Siddiqui, N. (2013). Antimicrobial activity of Schiff bases of coumarin-incorporated 1, 3, 4-oxadiazole derivatives: an in vitro evaluation. Medicinal Chemistry Research, 22(9), 4455-4458. https://doi.org/10.1007/s00044-012-0452-9
  • Bichra, M., & Benkhalti, F. (2012). Antioxidant and anti-browning activities of Mentha suaveolens extracts. African Journal of Biotechnology, 11(35), 8722-8729.
  • Bichra, M., El-Modafar, C., El-Abbassi, A., Bouamama, H., & Benkhalti, F. (2013). Antioxidant activities and phenolic profile of six Moroccan selected herbs. Journal of Microbiology, Biotechnology and Food Sciences, 2(4), 2320 2338. https://office2.jmbfs.org/index.php/JMBFS/article/view/7495
  • Bimakr, M., Rahman, R.A., Taip, F. S., Ganjloo, A., Salleh, L.M., Selamat, J., . . . Zaidul, I. (2011). Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food and bioproducts processing, 89(1), 67-72. https://doi.org/10.1016/j.fbp.2010.03.002
  • Bougatef, A., Hajji, M., Balti, R., Lassoued, I., Triki-Ellouz, Y., & Nasri, M. (2009). Antioxidant and free radical-scavenging activities of smooth hound (Mustelus mustelus) muscle protein hydrolysates obtained by gastrointestinal proteases. Food Chemistry, 114(4), 1198-1205. https://doi.org/10.1016/j.foodchem.2008.10.075
  • Božović, M., Pirolli, A., & Ragno, R. (2015). Mentha suaveolens Ehrh.(Lamiaceae) essential oil and its main constituent piperitenone oxide: Biological activities and chemistry. Molecules, 20(5), 8605-8633. https://doi.org/10.3390/molecules20058605
  • Bruneton, J. (2009). Pharmacognosie, phytochimie et plantes médicinales [Pharmacognosy, phytochemistry and medicinal plants]. 4e éd, revue et augmentée, Paris, Tec & Doc, Éditions médicales internationales, 1288p.
  • Chang, H.-J., Kim, H.J., & Chun, H.S. (2007). Quantitative structure− activity relationship (QSAR) for neuroprotective activity of terpenoids. Life sciences, 80(9), 835-841. https://doi.org/10.1016/j.lfs.2006.11.009
  • Dohou, R., Yamni, K., Tahrouch, S., Hassani, L.I., Badoc, A., & Gmira, N. (2003). Screening phytochimique d'une endémique iberomarocaine, Thymelaea lythroides [Phytochemical screening of an endemic iberomarocaine, Thymelaea lythroides]. Bulletin-Société de Pharmacie de Bordeaux, 142(1/4), 61-78.
  • Egan, D. O'kennedy, R., Moran, E., Cox, D., Prosser, E., Thornes, R.D. (1990). Drug Metabolism Reviews, 22(5), 503-529. https://doi.org/10.3109/03602539008991449
  • El-Kashoury, E.-S. A., El-Askary, H.I., Kandil, Z.A., Taher, E.E., & Salem, M.A. (2015). Molluscicidal and mosquitocidal activities of the essential oil of Mentha suaveolens Ehrh. cultivated in Egypt. Journal of Essential Oil Bearing Plants, 18(2), 436-443. https://doi.org/10.1080/0972060X.2013.793981
  • Fuller, R.W., Bokesch, H.R., Gustafson, K.R., Mckee, T.C., Cardellina II, J.H., Mcmahon, J.B., Cragg, G.M., Soejarto, D.D., & Boyd, M.R. (1994).HIV-Inhibitory Coumarins from Latex of the Tropical Rain Forest Tree Calophyllum teysmannii var. inophylloide. Bioorg. Med. Chem. Letters 4, 1961-1964.
  • Fujioka, T., Furumi, K., Fujii, H., Okabe, H., Mihashi, K., Nakano, Y., . . . Mori, M. (1999). Antiproliferative constituents from umbelliferae plants. V.A new furanocoumarin and falcarindiol furanocoumarin ethers from the root of Angelica japonica. Chemical and Pharmaceutical Bulletin, 47(1), 96-100. https://doi.org/10.1248/cpb.47.96
  • García-Argáez, A.N., Apan, T.O.R., Delgado, H.P., Velázquez, G., & Martínez-Vázquez, M. (2000). Anti-inflammatory activity of coumarins from Decatropis bicolor on TPA ear mice model. Planta medica, 66(03), 279-281. https://doi.org/10.1055/s-0029-1243137
  • Gill, M. (1993). Aromatic Compounds The Chemistry of Natural Products (pp. 60-105): Springer. https://doi.org/10.1007/978-94-011-2144-6_2
  • Hagerman AE, M.-H.I., Makkar HP. (2000). Quantification of Tannins in Tree Foliage. Vienna: Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, 26.
  • Harley, R., & Brighton, C. (1977). Chromosome numbers in the genus Mentha L. Botanical Journal of the Linnean Society, 74(1), 71 96. https://doi.org/10.1111/j.1095 8339.1977.tb01168.x
  • Hiermann, A., Schramm, H., & Laufer, S. (1998). Anti-inflammatory activity of myricetin-3-O-β-D-glucuronide and related compounds. Inflammation Research, 47(11), 421-427. https://doi.org/10.1007/s000110050355
  • Hmamouchi, M. (1999). Les plantes médicinales et aromatiques marocaines: utilisation, biologie, écologie, chimie, pharmacologie, toxicologie, lexiques.[ Moroccan medicinal and aromatic plants: use, biology, ecology, chemistry, pharmacology, toxicology, glossaries.] Bibliographie du patrimoine culturel immatériel, 1999.
  • Hsiou, Y.D., Hang, C.L., Che-Ming, T., Yang, C.W. (2000). Phytochemical and Pharmacological Studies on Chinese Paeonia Species. Journal of The Chinese Chemical Society, 47(2):381-388. https://doi.org/10.1002/JCCS.200000051
  • Jamila, F., & Mostafa, E. (2014). Ethnobotanical survey of medicinal plants used by people in Oriental Morocco to manage various ailments. Journal of Ethnopharmacology, 154(1), 76-87. https://doi.org/10.1016/j.jep.2014.03.016
  • Kabran G.R., N.C.A., J.A. Mamyrbékova-Békro, and Y.A. Békro. (2011). CCM d’extraits sélectifs de 10 plantes utilisées dans le traitement Traditionnel du Cancer du sein en Côte d’Ivoire. [TLC of selective extracts of 10 plants used in the traditional treatment of breast cancer in Côte d'Ivoire.]. Europ.J.Scientific.Research, 63(4), 592-603.
  • Kandaswamy, N., & Raveendiran, N. (2014). Synthesis, Characterization and Antifungal activity of Coumarin based random copolyester. American Journal of Research, 4(3), 704-707.
  • Karousou, R., Balta, M., Hanlidou, E., & Kokkini, S. (2007). “Mints”, smells and traditional uses in Thessaloniki (Greece) and other Mediterranean countries. Journal of ethnopharmacology, 109(2), 248-257. https://doi.org/10.1016/j.jep.2006.07.022
  • Kasrati, A., Alaoui Jamali, C., & Abbad, A. (2017). Antioxidant properties of various extract from selected wild Moroccan aromatic and medicinal species. Trends in Phytochemical Research, 1(4), 175-182. https://dorl.net/dor/20.1001.1.25883623.2017.1.4.1.8
  • Kofinas, C., Loukis, A., Harvala, C., Roussakis, C., Maillard, M., & Hostettmann, K. (1998). Cytotoxic coumarins from the aerial parts of Tordylium apulum and their effects on a non-small-cell bronchial carcinoma cell line. Planta medica, 64(02), 174-176. https://doi.org/10.1055/s-2006-957398
  • Kumar, P., Mishra, S., Malik, A., & Satya, S. (2011). Insecticidal properties of Mentha species: a review. Industrial Crops and Products, 34(1), 802-817.
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Details

Primary Language English
Subjects Plant Biochemistry, Structural Biology
Journal Section Articles
Authors

Moha Afrokh This is me 0000-0002-2872-9093

Kamal Boumhara This is me 0000-0002-2722-4376

Khalid Chatoui This is me 0000-0002-6065-9873

Saida Tahrouch This is me 0000-0002-3981-3864

Abdelhakim Hatimi This is me 0000-0001-6790-4440

Hicham Harhar 0000-0001-8266-0130

Mohamed Tabyaoui This is me 0000-0001-6556-5238

Early Pub Date July 31, 2023
Publication Date August 27, 2023
Submission Date July 27, 2022
Published in Issue Year 2023 Volume: 10 Issue: 3

Cite

APA Afrokh, M., Boumhara, K., Chatoui, K., Tahrouch, S., et al. (2023). Phytochemical screening and in vitro antioxidant activities of Mentha suaveolens Ehrh. extract. International Journal of Secondary Metabolite, 10(3), 332-344. https://doi.org/10.21448/ijsm.1148664
International Journal of Secondary Metabolite

e-ISSN: 2148-6905