Total polyphenols and antioxidant activity of yellow velvetleaf (Limnocharis flava) extract

Year 2023, Volume: 10 Issue: 4, 583 - 589, 01.12.2023

Sabri Sudirman , Mey Arianti , Gama Dian Nugroho , Sherly Ridhowati , Puspa Ayu Pitayati , Miftahul Janna

https://doi.org/10.21448/ijsm.1328150

Abstract

An increase in free radicals can cause damage to cells and tissues in the body. This is caused by a lack of antioxidant ability in the body, so it requires antioxidants outside the body. One of the plants that can be used as a source of antioxidants is the yellow velvetleaf (Limnocharis flava), which has a class of polyphenolic compounds. The crude extract of this plant still has other components that are not included in the polyphenol compound. This study aimed to measure the total polyphenol and flavonoid contents as well as the antioxidant activity of the yellow velvetleaf plant (L. flava) before and after purification. The purification process is carried out using the solid-phase extraction method. The antioxidant activity was determined by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The total polyphenol and flavonoid contents increase after the purification process. Therefore, the antioxidant activity of purified extract is also increased when compared to crude extract. These results show that the purification process successfully increased the levels of polyphenol compounds from the yellow velvetleaf plant and its antioxidant activity. Thus, the purified extract can be used as an alternative source of natural antioxidants and can be developed as a food supplement ingredient.

Keywords

Antioxidant, Limnocharis flava, Polyphenol, Purification, Yellow velvetleaf

References

• Baehaki, A.C.E., Lestari, S.D., & Siregar, N. (2019). Phytochemical compounds and antioxidant activity of yellow velvetleaf fruit (Limnocharis flava) extract. Asian Journal of Pharmaceutical and Clinical Research, 55-57. https://doi.org/10.22159/ajpcr.2020.v13i2.36136
• Chandra, S., Khan, S., Avula, B., Lata, H., Yang, M.H., Elsohly, M.A., & Khan, I.A. (2014). Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: A comparative study. Evidence-Based Complementary and Alternative Medicine, 2014, 253875. https://doi.org/10.1155/2014/253875
• Chew, Y.L., Lim, Y.Y., Omar, M., & Khoo, K.S. (2008). Antioxidant activity of three edible seaweeds from two areas in South East Asia. LWT - Food Science and Technology, 41(6), 1067-1072. https://doi.org/10.1016/j.lwt.2007.06.013
• Dai, J., & Mumper, R.J. (2010). Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10), 7313 7352. https://doi.org/10.3390/molecules15107313
• Dini, I., & Grumetto, L. (2022). Recent advances in natural polyphenol pesearch. Molecules, 27(24). https://doi.org/10.3390/molecules27248777
• Ighodaro, O.M., & Akinloye, O.A. (2019). First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine, 54(4), 287-293. https://doi.org/10.1016/j.ajme.2017.09.001
• Jamila, C.N.S.U., Chandra, B., Zulharmita, Z., & Rivai, H. (2021). The Ethnopharmacology, phytochemistry, pharmacology activities of yellow velvetleaf plant (Limnocharis flava): A review. International Journal of Pharmaceutical Sciences and Medicine, 6(5), 12-20. https://doi.org/10.47760/ijpsm.2021.v06i05.002
• Lee, C.Y., Nanah, C.N., Held, R.A., Clark, A.R., Huynh, U.G.T., Maraskine, M.C., . . . Sharma, A. (2015). Effect of electron donating groups on polyphenol-based antioxidant dendrimers. Biochimie, 111, 125-134. https://doi.org/10.1016/j.biochi.2015.02.001
• Matyas, M., Hasmasanu, M.G., & Zaharie, G. (2019). Antioxidant capacity of preterm neonates assessed by hydrogen donor value. Medicina, 55(11), 720. https://doi.org/10.3390/medicina55110720
• Nugroho, A.E., Malik, A., & Pramono, S. (2013). Total phenolic and flavonoid contents, and in vitro antihypertension activity of purified extract of Indonesian cashew leaves (Anacardium occidentale L.). International Food Research Journal, 20(1), 299-305.
• Panche, A.N., Diwan, A.D., & Chandra, S.R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5. https://doi.org/10.1017/jns.2016.41
• Pérez-Magariño, S., Ortega-Heras, M., & Cano-Mozo, E. (2008). Optimization of a solid-phase extraction method using copolymer sorbents for isolation of phenolic compounds in red wines and quantification by HPLC. Journal of Agricultural and Food Chemistry, 56(24), 11560-11570. https://doi.org/10.1021/jf802100j
• Phaniendra, A., Jestadi, D.B., & Periyasamy, L. (2015). Free radicals: Properties, sources, targets, and their implication in various diseases. Indian Journal of Clinical Biochemistry, 30(1), 11-26. https://doi.org/10.1007/s12291-014-0446-0
• Roehrs, M., Valentini, J., Paniz, C., Moro, A., Charão, M., Bulcão, R., Freitas, F., Brucker, N., Duarte, M., Leal, M., Burg, G., Grune, T., & Garcia, S.C. (2011). The relationships between exogenous and endogenous antioxidants with the lipid profile and oxidative damage in hemodialysis patients. BMC Nephrology, 12(1), 59. https://doi.org/10.1186/1471-2369-12-59
• Sudirman, S., Janna, M., Herpandi, & Widiastuti, I. (2022). In vitro inhibitory HMG-CoA reductase activity of purified polyphenol compounds from water lettuce (Pistia stratiotes) leaf extract. Tropical Journal of Natural Product Research, 6(7), 1131-1134. https://doi.org/10.26538/tjnpr/v6i7.15
• Swallah, M.S., Sun, H., Affoh, R., Fu, H., & Yu, H. (2020). Antioxidant potential overviews of secondary metabolites (Polyphenols) in fruits. International Journal of Food Science, 2020, 1-8. https://doi.org/10.1155/2020/9081686
• Valencia-Avilés, E., García-Pérez, M., Garnica-Romo, M., Figueroa-Cárdenas, J., Meléndez-Herrera, E., Salgado-Garciglia, R., & Martínez-Flores, H. (2018). Antioxidant properties of polyphenolic extracts from Quercus laurina, Quercus crassifolia, and Quercus scytophylla bark. Antioxidants, 7(7). https://doi.org/10.3390/antiox7070081
• Wang, X., Wang, S., Huang, S., Zhang, L., Ge, Z., Sun, L., & Zong, W. (2019). Purification of polyphenols from distiller’s grains by macroporous resin and analysis of the polyphenolic components. Molecules, 24(7). https://doi.org/10.3390/molecules24071284
• Yi, W., & Wetzstein, H.Y. (2011). Effects of drying and extraction conditions on the biochemical activity of selected herbs. HortScience, 46(1), 70 73. https://doi.org/10.21273/hortsci.46.1.70
• Zeka, K., Ruparelia, K., Arroo, R., Budriesi, R., & Micucci, M. (2017). Flavonoids and their metabolites: Prevention in cardiovascular diseases and diabetes. Diseases, 5(3). https://doi.org/10.3390/diseases5030019