Araştırma Makalesi
BibTex RIS Kaynak Göster

Molluscicidal activity of Nigella sativa seed, Azadirachta indica leaf and Khaya senegalensis bark ethanolic extracts against Bulinus truncatus snails

Yıl 2024, Cilt: 11 Sayı: 3, 574 - 583, 29.08.2024
https://doi.org/10.21448/ijsm.1330090

Öz

In order to control schistosomiasis, a strategy involves eliminating the intermediate host responsible for its transmission. Plant-derived molluscicides have been extensively researched as a cost-effective approach to combat this disease, seeking alternative solutions to synthetic molluscicides. The study aimed to analyze the phytoconstituents and assess the molluscicidal impact of ethanolic extracts on adult Bulinus truncatus snails. Dried plant materials were ground into powder, and then extracted using ethanol 96%. Preliminary phytochemical screening was carried out using standard procedures. In vitro molluscicidal activity was evaluated for the three plants extracts in different concentrations (125, 250, 500 and 1000 ppm). Every 5 adult snails were placed in a plastic cup containing a specific amount of dechlorinated water with plant sample. Control negative was prepared by placing the snails in dechlorinated water only, the numbers of survived and dead snails were recorded after 24 and 48 hours. The Lethal dose and Standard deviation were calculated. The phytochemical screening revealed the presence of different chemical constituents in the three plants samples. The result showed that all the plants extracts possessed molluscicidal activity against B. truncatus snails. The A. indica leaf extract was the most effective with LD50 of 74.32544 ppm. From the findings of this study, Molluscicide of plant origin could be useful against the common snail species in Sudan; therefore, the selected plants can play a big role in community based schistosomiasis control.

Kaynakça

  • Abdullah, N.S., Aziz, N.A., Mailon, R. (2017). Molluscicidal activity of Entada rheedii stem bark methanolic extract against paddy pest Pomacea canaliculata (GOLDEN APPLE SNAIL). Malaysian Journal of Analytical Sciences, 21(1), 46-5. http://dx.doi.org/10.17576/mjas-2017-2101-06
  • Abo-Zeid, K., Shohayeb, M. (2015). Evaluation of the biocidal activity of alkaloids, saponins and volatile oil extracted from Nigella sativa seeds against miracidia and cercariae of Schistosoma mansoni. International Journal of Pharmaceutical Science Invention, 4(1), 47-54.
  • Adamu, I., Majiyebo, A.J., Alozieuwa, U.B., Lawal, A.A., Alawode, B.O.O.R.A., & Berinyuy, E.B (2022). Ethnopharmacology, chemical constituents, and biological activities of Khaya senegalensis (Desr.) A. Juss a forest plant with multi-purpose applications. AROC in Natural Products Research, 2(2), 10-15. https://doi.org/10.53858/arocnpr02021015
  • Ahmed, A.A. (2006). Schistosomiasis in sugar cane schemes, Sudan. Sudan J Nat Sci, 4, 1-11. Amin, M., & Abubaker, H. (2017). Control of schistosomiasis in the Gezira irrigation scheme, Sudan. Journal of Biosocial Science, 49(1), 83 98. https://doi.org/10.1017/s0021932016000079
  • Ayi, Fabrice Chandre, I., Coelho, P.M.Z., El-Harawy, A.K., Elemam, M.A., et al. (2019), Guidelines for laboratory and field testing of molluscicides for control of schistosomiasis. Rajpal Singh Yadav. World Health Organization, 978 92 4 151540 5. hal-02378870. https://hal.science/hal-02378870
  • Barua, A., Williams, C.D., & Ross, J.L. (2021). A literature review of biological and bio- rational control strategies for slugs: current research and future prospects. Insects, 12(6), 541. https://doi.org/10.3390/insects12060541
  • Dai, L., Wang, W., Dong, X., Hu, R., Nan, X. (2011). Molluscicidal activity of cardiac glycosides from Nerium indicum against Pomacea canaliculata and its implications for the mechanisms of toxicity. Environmental Toxicology and Pharmacology, 32(2), 226-32. https://doi.org/10.1016/j.etap.2011.05.007
  • Elbasheir, M.M., Karti, I.A., & Elamin, E.M. (2020). Evaluation of a rapid diagnostic test for Schistosoma mansoni infection based on the detection of circulating cathodic antigen in urine in Central Sudan. PLoS Neglected Tropical Diseases, 14(6), e0008313. https://doi.org/10.1371/journal.pntd.0008313
  • El-Seedi, H.R., Khalifa, S.A., Mohamed, A.H., Yosri, N., Zhao, C., El-Wakeil, N., & Verpoorte, R. (2022). Plant extracts and compounds for combating schistosomiasis. Phytochemistry Reviews, 1-116. https://doi.org/10.1007/s11101-022-09836-x
  • Esquilla, M.G., Sanchez, C.B., Tenorio, J.C. (2021). Molluscicidal activity of Makabuhay (Tinospora rumphii Boerl) stem ethanolic extract against Radix (Lymnaea) spp. Snails. Journal on New Biological Reports, 10(2), 64 71.
  • Evans, W.C. (2009). Trease and evans Pharmacognosy. 9th Edition published by Saunders Elsevier. 553.
  • Hajissa, K., Muhajir, A.E., Eshag, H.A., Alfadel, A., Nahied, E., Dahab, R., ... & Mohamed, Z. (2018). Prevalence of schistosomiasis and associated risk factors among school children in Um Asher Area, Khartoum, Sudan. BMC Research Notes, 11(1), 1 5. https://doi.org/10.1186/s13104-018-3871-y
  • Islam, M.T., Khan, M.R., & Mishra, S.K. (2019). An updated literature-based review: Phytochemistry, pharmacology and therapeutic promises of Nigella sativa L. Oriental Pharmacy and Experimental Medicine, 19, 115-129. https://doi.org/10.1007/s13596-019-00363-3
  • Kanu Ikechukwu, K., Ndubisi, E.J.O., & Nwadialor, K.L. (2021). African Religion And Culture Honoring the Past and shaping the future. First Edition. Editors: Kanu, I.A., Nwadialor, K.L., Ndubisi, E.J.O., Innovative trends in African traditional medicine (pp. 45-64). Association for the Promotion of African Studies, Maryland.
  • Karunamoorthi, K., Almalki, M.J., & Ghailan, K.Y. (2018). Schistosomiasis: A neglected tropical disease of poverty: A call for intersectoral mitigation strategies for better health. Journal of Health Research and Reviews, 5(1), 1 12. https://doi.org/10.4103/jhrr.jhrr_92_17
  • Kashyap, S., Khagta, S., Guleria, K., & Arya, V. (2019). Plants as Molluscicides: A recent update. International Journal of Botany Studies, 4(1), 50-56.
  • Ke, W., Lin, X., Yu, Z., Sun, Q., Zhang, Q. (2017). Molluscicidal activity and physiological toxicity of Macleaya cordata alkaloids components on snail Oncomelania hupensis. Pesticide Biochemistry and Physiology, 143, 111 115. https://pubmed.ncbi.nlm.nih.gov/29183578/
  • Ke, W., Tu, C., Cao, D., Lin, X., Sun, Q., & Zhang, Q. (2019). Molluscicidal activity and physiological toxicity of quaternary benzo [c] phenanthridine alkaloids (QBAs) from Macleaya cordata fruits on Oncomelania hupensis. PLoS Neglected Tropical Diseases, 13(10), e0007740. https://doi.org/10.1371/journal.pntd.0007740
  • Mutamad, A., Mohamed, K., Noma, M., Hwiada, A., Algali, M., & Mamoun, H. (2016). The Transmission Patterns of Shistosomiasis in Khartoum State, Sudan. Annals of Clinical Pathology, 4(6), 1088.
  • Mohamed, Issam A.W. (February 27, 2012). Water Borne Diseases and Rural Development Study of Bilharzias in Gezira Irrigated Agricultural Scheme, Sudan Available at SSRN: http://dx.doi.org/10.2139/ssrn.2011943
  • Mtemeli, F. L., Walter, I., Tinago, T., & Shoko, R. (2021). An assessment of the molluscicidal potential of Cucurbita maxima seed extracts on Biomphalaria pfeifferi and Bulinus globosus snails. All Life, 14(1), 244-255. https://doi.org/10.1080/26895293.2021.1901788
  • Mwonga, K.B, Waniki, N.E, Dorcas Y.S, Piero, N.M (2015). Molluscicidal effects of aqueous extracts of selected medicinal plants from Makueni County, Kenya. Pharm Anal Acta., 6(11), 445-59. https://doi.org/10.4172/2153-2435.1000445
  • Nigam, M., Mishra, A.P., Adhikari‐Devkota, A., Dirar, AI., Hassan, MM, Adhikari, A., Belwal T., Devkota, H.P. (2020). Fruits of Terminalia chebula Retz.: A review on traditional uses, bioactive chemical constituents and pharmacological activities. Phytotherapy Research, 34(10), 2518-2533. https://doi.org/10.1002/ptr.6702
  • Noorshilawati, A.A., Suraya, A., & Rossiyah, S.S. (2020). Molluscicidal activity of Ipomoea batatas leaf extracts against Pomacea canaliculata (Golden apple snail). Food Research, 4(5), 131-137. https://doi.org/10.26656/fr.2017
  • Piyasena, K.G.N.P., & Qader, M.M. (2022). Chemistry of Natural Products: Phytochemistry and Pharmacognosy of Medicinal Plants. Berlin, Boston: De Gruyter, 2022. https://doi.org/10.1515/9783110595949
  • Qian, Y., & Zhou, X. (2021). Schistosomiasis Risk and Prevention. In Prevention and Control of Infectious Diseases in BRI Countries, Springer Singapore, 143 156. https://www.infontd.org/resource/schistosomiasis-risk-and-prevention
  • Quintero Santos, C.D., Castellanos, L., & Becerra-Rozo, W.M. (2022). Possibilities of the alternative management of mollusk pests in agricultural crops. A review. INGE CUC, 18(1), 1-13. http://doi.org/10.17981/ingecuc.18.1.2022.01
  • Rinaldo, D., Perez-Saez, J., Vounatsou, P., Utzinger, J., & Arcand, J.L. (2021). The economic impact of schistosomiasis. Infectious Diseases of Poverty, 10(1), 1 12. https://doi.org/10.1186/s40249-021-00919-z
  • Satti, A.B., Mohammed, W.M., Garbi, M.I., & Hamad, M.N.M. (2022). Prevalence of Schistosoma Haematobium Infection in Alsyal Village, River Nile State, Sudan. SAR Journal of Pathology and Microbiology, 3(6), 81 84. https://doi.org/10.36346/sarjpm.2022.v03i06.001
  • Schenkel, E.P., Gosmann, G., Athayde, M.L. (2007). Saponinas. In: Farmacognosia: da planta ao medicamento. 6ª Porto Alegre: Editora da UFRGS; Florianópolis: Editora da UFSC, 711–740.
  • Sulieman, Y., Eltayeb, R., Pengsakul, T., Afifi, A., Zakaria, M., & Khairala, M. (2017). Schistosomiasis as a disease and its prevalence in Sudan: An overview. Journal of Coastal Life Medicine, 5(3), 129-133. https://doi.org/10.12980/jclm.5.2017J6-247
  • Thétiot‐Laurent, S.A.L., Boissier, J., Robert, A., & Meunier, B. (2013). Schistosomiasis chemotherapy. Angewandte Chemie International Edition, 52(31), 7936 7956. https://doi.org/10.1002/anie.201208390
  • Verjee, M.A. (2019). Schistosomiasis: still a cause of significant morbidity and mortality. Research and Reports in Tropical Medicine, 10, 153 163. https://doi.org/10.2147/RRTM.S204345
  • World Health Organization. (1994). Assessment of fracture risk and its application to screening for postmenopausal osteoporosis: report of a WHO study group [meeting held in Rome from 22 to 25 June 1992].

Molluscicidal activity of Nigella sativa seed, Azadirachta indica leaf and Khaya senegalensis bark ethanolic extracts against Bulinus truncatus snails

Yıl 2024, Cilt: 11 Sayı: 3, 574 - 583, 29.08.2024
https://doi.org/10.21448/ijsm.1330090

Öz

In order to control schistosomiasis, a strategy involves eliminating the intermediate host responsible for its transmission. Plant-derived molluscicides have been extensively researched as a cost-effective approach to combat this disease, seeking alternative solutions to synthetic molluscicides. The study aimed to analyze the phytoconstituents and assess the molluscicidal impact of ethanolic extracts on adult Bulinus truncatus snails. Dried plant materials were ground into powder, and then extracted using ethanol 96%. Preliminary phytochemical screening was carried out using standard procedures. In vitro molluscicidal activity was evaluated for the three plants extracts in different concentrations (125, 250, 500 and 1000 ppm). Every 5 adult snails were placed in a plastic cup containing a specific amount of dechlorinated water with plant sample. Control negative was prepared by placing the snails in dechlorinated water only, the numbers of survived and dead snails were recorded after 24 and 48 hours. The Lethal dose and Standard deviation were calculated. The phytochemical screening revealed the presence of different chemical constituents in the three plants samples. The result showed that all the plants extracts possessed molluscicidal activity against B. truncatus snails. The A. indica leaf extract was the most effective with LD50 of 74.32544 ppm. From the findings of this study, Molluscicide of plant origin could be useful against the common snail species in Sudan; therefore, the selected plants can play a big role in community based schistosomiasis control.

Kaynakça

  • Abdullah, N.S., Aziz, N.A., Mailon, R. (2017). Molluscicidal activity of Entada rheedii stem bark methanolic extract against paddy pest Pomacea canaliculata (GOLDEN APPLE SNAIL). Malaysian Journal of Analytical Sciences, 21(1), 46-5. http://dx.doi.org/10.17576/mjas-2017-2101-06
  • Abo-Zeid, K., Shohayeb, M. (2015). Evaluation of the biocidal activity of alkaloids, saponins and volatile oil extracted from Nigella sativa seeds against miracidia and cercariae of Schistosoma mansoni. International Journal of Pharmaceutical Science Invention, 4(1), 47-54.
  • Adamu, I., Majiyebo, A.J., Alozieuwa, U.B., Lawal, A.A., Alawode, B.O.O.R.A., & Berinyuy, E.B (2022). Ethnopharmacology, chemical constituents, and biological activities of Khaya senegalensis (Desr.) A. Juss a forest plant with multi-purpose applications. AROC in Natural Products Research, 2(2), 10-15. https://doi.org/10.53858/arocnpr02021015
  • Ahmed, A.A. (2006). Schistosomiasis in sugar cane schemes, Sudan. Sudan J Nat Sci, 4, 1-11. Amin, M., & Abubaker, H. (2017). Control of schistosomiasis in the Gezira irrigation scheme, Sudan. Journal of Biosocial Science, 49(1), 83 98. https://doi.org/10.1017/s0021932016000079
  • Ayi, Fabrice Chandre, I., Coelho, P.M.Z., El-Harawy, A.K., Elemam, M.A., et al. (2019), Guidelines for laboratory and field testing of molluscicides for control of schistosomiasis. Rajpal Singh Yadav. World Health Organization, 978 92 4 151540 5. hal-02378870. https://hal.science/hal-02378870
  • Barua, A., Williams, C.D., & Ross, J.L. (2021). A literature review of biological and bio- rational control strategies for slugs: current research and future prospects. Insects, 12(6), 541. https://doi.org/10.3390/insects12060541
  • Dai, L., Wang, W., Dong, X., Hu, R., Nan, X. (2011). Molluscicidal activity of cardiac glycosides from Nerium indicum against Pomacea canaliculata and its implications for the mechanisms of toxicity. Environmental Toxicology and Pharmacology, 32(2), 226-32. https://doi.org/10.1016/j.etap.2011.05.007
  • Elbasheir, M.M., Karti, I.A., & Elamin, E.M. (2020). Evaluation of a rapid diagnostic test for Schistosoma mansoni infection based on the detection of circulating cathodic antigen in urine in Central Sudan. PLoS Neglected Tropical Diseases, 14(6), e0008313. https://doi.org/10.1371/journal.pntd.0008313
  • El-Seedi, H.R., Khalifa, S.A., Mohamed, A.H., Yosri, N., Zhao, C., El-Wakeil, N., & Verpoorte, R. (2022). Plant extracts and compounds for combating schistosomiasis. Phytochemistry Reviews, 1-116. https://doi.org/10.1007/s11101-022-09836-x
  • Esquilla, M.G., Sanchez, C.B., Tenorio, J.C. (2021). Molluscicidal activity of Makabuhay (Tinospora rumphii Boerl) stem ethanolic extract against Radix (Lymnaea) spp. Snails. Journal on New Biological Reports, 10(2), 64 71.
  • Evans, W.C. (2009). Trease and evans Pharmacognosy. 9th Edition published by Saunders Elsevier. 553.
  • Hajissa, K., Muhajir, A.E., Eshag, H.A., Alfadel, A., Nahied, E., Dahab, R., ... & Mohamed, Z. (2018). Prevalence of schistosomiasis and associated risk factors among school children in Um Asher Area, Khartoum, Sudan. BMC Research Notes, 11(1), 1 5. https://doi.org/10.1186/s13104-018-3871-y
  • Islam, M.T., Khan, M.R., & Mishra, S.K. (2019). An updated literature-based review: Phytochemistry, pharmacology and therapeutic promises of Nigella sativa L. Oriental Pharmacy and Experimental Medicine, 19, 115-129. https://doi.org/10.1007/s13596-019-00363-3
  • Kanu Ikechukwu, K., Ndubisi, E.J.O., & Nwadialor, K.L. (2021). African Religion And Culture Honoring the Past and shaping the future. First Edition. Editors: Kanu, I.A., Nwadialor, K.L., Ndubisi, E.J.O., Innovative trends in African traditional medicine (pp. 45-64). Association for the Promotion of African Studies, Maryland.
  • Karunamoorthi, K., Almalki, M.J., & Ghailan, K.Y. (2018). Schistosomiasis: A neglected tropical disease of poverty: A call for intersectoral mitigation strategies for better health. Journal of Health Research and Reviews, 5(1), 1 12. https://doi.org/10.4103/jhrr.jhrr_92_17
  • Kashyap, S., Khagta, S., Guleria, K., & Arya, V. (2019). Plants as Molluscicides: A recent update. International Journal of Botany Studies, 4(1), 50-56.
  • Ke, W., Lin, X., Yu, Z., Sun, Q., Zhang, Q. (2017). Molluscicidal activity and physiological toxicity of Macleaya cordata alkaloids components on snail Oncomelania hupensis. Pesticide Biochemistry and Physiology, 143, 111 115. https://pubmed.ncbi.nlm.nih.gov/29183578/
  • Ke, W., Tu, C., Cao, D., Lin, X., Sun, Q., & Zhang, Q. (2019). Molluscicidal activity and physiological toxicity of quaternary benzo [c] phenanthridine alkaloids (QBAs) from Macleaya cordata fruits on Oncomelania hupensis. PLoS Neglected Tropical Diseases, 13(10), e0007740. https://doi.org/10.1371/journal.pntd.0007740
  • Mutamad, A., Mohamed, K., Noma, M., Hwiada, A., Algali, M., & Mamoun, H. (2016). The Transmission Patterns of Shistosomiasis in Khartoum State, Sudan. Annals of Clinical Pathology, 4(6), 1088.
  • Mohamed, Issam A.W. (February 27, 2012). Water Borne Diseases and Rural Development Study of Bilharzias in Gezira Irrigated Agricultural Scheme, Sudan Available at SSRN: http://dx.doi.org/10.2139/ssrn.2011943
  • Mtemeli, F. L., Walter, I., Tinago, T., & Shoko, R. (2021). An assessment of the molluscicidal potential of Cucurbita maxima seed extracts on Biomphalaria pfeifferi and Bulinus globosus snails. All Life, 14(1), 244-255. https://doi.org/10.1080/26895293.2021.1901788
  • Mwonga, K.B, Waniki, N.E, Dorcas Y.S, Piero, N.M (2015). Molluscicidal effects of aqueous extracts of selected medicinal plants from Makueni County, Kenya. Pharm Anal Acta., 6(11), 445-59. https://doi.org/10.4172/2153-2435.1000445
  • Nigam, M., Mishra, A.P., Adhikari‐Devkota, A., Dirar, AI., Hassan, MM, Adhikari, A., Belwal T., Devkota, H.P. (2020). Fruits of Terminalia chebula Retz.: A review on traditional uses, bioactive chemical constituents and pharmacological activities. Phytotherapy Research, 34(10), 2518-2533. https://doi.org/10.1002/ptr.6702
  • Noorshilawati, A.A., Suraya, A., & Rossiyah, S.S. (2020). Molluscicidal activity of Ipomoea batatas leaf extracts against Pomacea canaliculata (Golden apple snail). Food Research, 4(5), 131-137. https://doi.org/10.26656/fr.2017
  • Piyasena, K.G.N.P., & Qader, M.M. (2022). Chemistry of Natural Products: Phytochemistry and Pharmacognosy of Medicinal Plants. Berlin, Boston: De Gruyter, 2022. https://doi.org/10.1515/9783110595949
  • Qian, Y., & Zhou, X. (2021). Schistosomiasis Risk and Prevention. In Prevention and Control of Infectious Diseases in BRI Countries, Springer Singapore, 143 156. https://www.infontd.org/resource/schistosomiasis-risk-and-prevention
  • Quintero Santos, C.D., Castellanos, L., & Becerra-Rozo, W.M. (2022). Possibilities of the alternative management of mollusk pests in agricultural crops. A review. INGE CUC, 18(1), 1-13. http://doi.org/10.17981/ingecuc.18.1.2022.01
  • Rinaldo, D., Perez-Saez, J., Vounatsou, P., Utzinger, J., & Arcand, J.L. (2021). The economic impact of schistosomiasis. Infectious Diseases of Poverty, 10(1), 1 12. https://doi.org/10.1186/s40249-021-00919-z
  • Satti, A.B., Mohammed, W.M., Garbi, M.I., & Hamad, M.N.M. (2022). Prevalence of Schistosoma Haematobium Infection in Alsyal Village, River Nile State, Sudan. SAR Journal of Pathology and Microbiology, 3(6), 81 84. https://doi.org/10.36346/sarjpm.2022.v03i06.001
  • Schenkel, E.P., Gosmann, G., Athayde, M.L. (2007). Saponinas. In: Farmacognosia: da planta ao medicamento. 6ª Porto Alegre: Editora da UFRGS; Florianópolis: Editora da UFSC, 711–740.
  • Sulieman, Y., Eltayeb, R., Pengsakul, T., Afifi, A., Zakaria, M., & Khairala, M. (2017). Schistosomiasis as a disease and its prevalence in Sudan: An overview. Journal of Coastal Life Medicine, 5(3), 129-133. https://doi.org/10.12980/jclm.5.2017J6-247
  • Thétiot‐Laurent, S.A.L., Boissier, J., Robert, A., & Meunier, B. (2013). Schistosomiasis chemotherapy. Angewandte Chemie International Edition, 52(31), 7936 7956. https://doi.org/10.1002/anie.201208390
  • Verjee, M.A. (2019). Schistosomiasis: still a cause of significant morbidity and mortality. Research and Reports in Tropical Medicine, 10, 153 163. https://doi.org/10.2147/RRTM.S204345
  • World Health Organization. (1994). Assessment of fracture risk and its application to screening for postmenopausal osteoporosis: report of a WHO study group [meeting held in Rome from 22 to 25 June 1992].
Toplam 34 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Farmasotik Botanik
Bölüm Makaleler
Yazarlar

Rawan Nugdalla 0009-0005-9972-5152

Ayat Alrasheid 0000-0002-3813-3656

Ahmed Saeed Kabbashi 0000-0003-3439-096X

Gokhan Zengin 0000-0001-6548-7823

Saad Mohammed Hussein Ayoub Bu kişi benim 0009-0006-6866-4534

Erken Görünüm Tarihi 6 Ağustos 2024
Yayımlanma Tarihi 29 Ağustos 2024
Gönderilme Tarihi 19 Temmuz 2023
Yayımlandığı Sayı Yıl 2024 Cilt: 11 Sayı: 3

Kaynak Göster

APA Nugdalla, R., Alrasheid, A., Kabbashi, A. S., Zengin, G., vd. (2024). Molluscicidal activity of Nigella sativa seed, Azadirachta indica leaf and Khaya senegalensis bark ethanolic extracts against Bulinus truncatus snails. International Journal of Secondary Metabolite, 11(3), 574-583. https://doi.org/10.21448/ijsm.1330090
International Journal of Secondary Metabolite
e-ISSN: 2148-6905