Separating Mad Honey from Other Honeys with Grayanotoxin Analysis in LC-MS/MS

Abstract

Objective: Honey samples were collected from 82 different pieces during the season when rhododendron flowers bloomed in Duzce areas in 2017. Since the majority of these honeys collected are sold as mad honey by local people, has been determined that it is the most suitable period to make their analysis. Material-Method: To apply our sample preparation method, honey samples were prepared to be analyzed on the LC-MS/MS. The accuracy, repeatability and reliability of the analysis method were provided by us. Results: The obtained results were calculated with the calibration plot drawn at ppb (ng/ml) level in LC-MS/MS. The grayanotoxin -III levels in mad honey were found to be % 36 for 0 ng/ml, %43 for 0.5-10 ng/ml, %15 for 10-50 ng/ml and %6 for 50≥ ng/ml. Conclusion: Most of honey samples do not contain toxins. Some of them are safe to use in middle proportions and less of them dangerous to consume.

Keywords

• Grayanotoxin-III
• Mad-Honey
• LC-MS/MS

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