In this study, the WST-1 cell proliferation test protocol was applied to examine the cytotoxicity of 19 different products on the market belonging to AYM-net® in L929 cell lines and the results were evaluated according to Elisa microplate reading data. The products were also tested at concentrations of 0.02, 0.03, 0.04, 0.05, 0.06 µg / ml, taking into account the amounts of daily use.
Results: When the results were evaluated, 19 different AYM-net® food supplement products sold on the market were cytotoxic in the L929 cell line, in the concentration range of 0.02-0.06 µg/mL, it was observed that the products were not toxic at these doses
Conclusion: As a result, different doses of AYM-net® herbal products did not show toxicity on L929 cells. Doses used are within the reliable range.
Keywords: L929, cell culture, cytotoxicity, food supplement, herbal mixture
L929, CELL CULTURE, CYTOTOXİCİTY, FOOD SUPPLEMENT, HERBAL MİXTURE