Yıl 2020, Cilt 1 , Sayı 3, Sayfalar 131 - 136 2020-12-15

Objective: Medicinal plants and products obtained from medicinal plants are widely used in the treatment of various diseases in our country as well as all over the world. However, scientific data on the biological effects and mechanisms of action of most extracts from medicinal plants are still lacking. Because of that interest in scientific research of the biological effects of medicinal plants and products obtained from medicinal plants is increasing day by day. In addition, the reliability of these products in terms of use apart from their effectiveness is very important for public health. In this study, we aimed to investigate the cytotoxicity of commercially marketed AYM-net® natural herbal food supplements in L929 (mouse fibroblast) cells. Material-Method: In this study, the WST-1 cell proliferation test protocol was applied to examine the cytotoxicity of 19 different products on the market belonging to AYM-net® in L929 cell lines and the results were evaluated according to Elisa microplate reading data. The products were also tested at concentrations of 0.02, 0.03, 0.04, 0.05, 0.06 µg / ml, taking into account the amounts of daily use. Results: When the results were evaluated, 19 different AYM-net® food supplement products sold on the market were cytotoxic in the L929 cell line, in the concentration range of 0.02-0.06 µg/mL, it was observed that the products were not toxic at these doses Conclusion: As a result, different doses of AYM-net® herbal products did not show toxicity on L929 cells. Doses used are within the reliable range. Keywords: L929, cell culture, cytotoxicity, food supplement, herbal mixture
L929, CELL CULTURE, CYTOTOXİCİTY, FOOD SUPPLEMENT, HERBAL MİXTURE
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Birincil Dil en
Konular Tamamlayıcı ve Entegre Tıp
Bölüm Araştırma Makaleleri
Yazarlar

Orcid: 0000-0002-9277-0648
Yazar: Pınar AĞYAR YOLDAŞ (Sorumlu Yazar)
Kurum: Düzce Üniversitesi Geleneksel ve Tamamlayıcı Tıp Uygulama ve Araştırma Merkezi
Ülke: Turkey


Orcid: 0000-0002-0209-312X
Yazar: Taner YOLDAŞ
Kurum: Düzce Üniversitesi Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
Ülke: Turkey


Orcid: 0000-0001-8915-3545
Yazar: Nisa SİPAHİ
Kurum: Düzce Üniversitesi Geleneksel ve Tamamlayıcı Tıp Uygulama ve Araştırma Merkezi
Ülke: Turkey


Tarihler

Başvuru Tarihi : 13 Kasım 2020
Kabul Tarihi : 8 Aralık 2020
Yayımlanma Tarihi : 15 Aralık 2020

AMA Ağyar Yoldaş P , Yoldaş T , Sipahi N . Cytotoxicity of Some Retail Food Supplements. International Journal of Traditional and Complementary Medicine Research. 2020; 1(3): 131-136.