The paraldehyde fuchsin positive material in corpora cardiaca of Pimpla turionellae L. (Hymenoptera:Ichneumonidae) during egg maturation

Abstract

The purpose of this study is to examine the effects of the neurosecretory material in corpus cardiacum (CC) on the reproduction of endoparasitic Pimpla turionellae L. For this purpose, the egg maturation and amount of the neurosecretory material in corpus cardiacum of the insects were examined by the serial cross sections. Egg maturation was determined by measuring the terminal oocyte length in the serial cross sections of the ovariole. The egg growth which has observed on the 3^rd day, reached the maximum on 15^th day and after that day the egg laying was observed. The sections of insect brains were stained by paraldehyde fuchsin. The amount of the paraldehyde fuchsin positive neurosecretory (PF⁺NS) material in CC was at a certain level in the first day of egg development. While terminal oocyte was reaching the maximum length, the amount of the PF⁺NS material in CC decreased to the minimum level. During the egg laying phase, the amount of the PF⁺NS material in CC reached the maximum level. These observations give us the idea that the neurosecretory material in corpus cardiacum of this insect may be related to the egg development.

Keywords

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Pimpla turionellae L.’nin (Hymenoptera:Ichneumonidae) yumurta gelişimi sırasında Korpora Kardiaka’sındaki paraldehit fuksin pozitif materyal

Abstract

Bu çalışmanın amacı, bir dış parazit olan Pimpla turionellae’nin korpus kardiacum(CC)’undaki nörosekresyon maddesinin üreme üzerine olan etkisini incelemektir. Bu amaçla, seri kesitlerde, böceğin yumurta gelişimi ve korpus kardiacum’undaki nörosekresyon maddesi gözlenmiştir.Böceğin beyin kesitleri paraldehit fuksin ile boyanmıştır. Yumurta gelişimi, yumurtalık kesitlerindeki terminal oosit boyu ölçülerek belirlenmiştir. 3. günde gözlenen yumurta büyümesi, 15. günde en yüksek seviyesine ulaşmış ve bu günden sonra yumurta bırakma gözlenmiştir. CC’deki paraldehit fuksin pozitif (PF+ NS) maddenin miktarı, yumurta gelişiminin ilk günlerinde belirli bir seviyedeydi. Terminal oositler en uzun boylarına ulaşırken, CC’deki PF+ NS maddenin seviyesi en aza indi. Yumurta bırakma evresinde ise bu seviye, en yüksek değerine ulaştı. Bu gözlemler bize, bu böceğin korpora kardiaka’sındaki nörosekresyon maddesinin yumurta gelişimi ile ilişkisi olabileceği fikrini vermiştir

Keywords

• Pimpla turionellae, Korpora kardiaca, Yumurta gelişimi, Nörosekresyon maddesi, Paraldehit fuksin

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