Antioxidant and antibacterial properties of a lichen species Diploschistes scruposus (Schreb.) Norman

Abstract

In this study, antioxidant and antibacterial activities of ethanol extract of Diploschistes scruposus (Schreb.) Norman (Graphidaceae) were investigated. Antioxidant activity of ethanol extract of D. Scruposus was investigated by five different methods: DPPH (2,2-diphenyl-1-picryl-hydrazyl ) radical scavenging activity, ABTS.+ (2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging activity, reducing power and determination of total phenolic contents. Different antioxidant activities of the extract were studied in comparison to known antioxidants. 50% inhibition concentration (IC50) values for lichen extract was observed 54.37±0.71 and 45.45±0.80 in DPPH assay and ABTS.+ assay, respectively. There were 161.4±0.01 µg/mL gallic acid equivalent of total phenolic compounds in the 1 mg of ethanol extract of D. scruposus. Antibacterial activity of lichen extract was determined by agar well diffusion method. The maximum antibacterial activity was observed against Bacillus subtilis and the minimum antibacterial activity was observed against Escherichia coli. This study shows that ethanol extract of D. scruposus can be used as an accessible source of natural antioxidant and antibacterial agent.

Keywords

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Diploschistes scruposus (Schreb.) Norman likeninin antioksidan ve antibakteriyal özellikleri

Abstract

Bu çalışmada, Diploschistes scruposus (Schreb.) Norman (Graphidaceae) likeninin antibakteriyal ve antioksidan özellikleri araştırılmıştır. D. scruposus likeninin etanol ekstresinin antioksidan aktivitesi beş farklı metodla çalışılmıştır: DPPH (2,2-difenil-1-pikrilhidrazil) radikal süpürme aktivitesi, ABTS.+ (2,2'-azino-bis (3etilbenzotiyazolin-6-sülfonik asit) radikali süpürme aktivitesi, indirgeme gücü ve toplam fenolik içeriğinin belirlenmesi. Test ekstresinin değişik antioksidan aktiviteleri bilinen antioksidanlarla kıyaslanmıştır. Liken ekstresinin %50 inhibisyon konsantrasyon değerleri (IC50), DPPH radikali süpürme aktivitesi için 54.37±0.71; ABTS.+ radikali süpürme aktivitesi için 45.45±0.80 olarak gözlenmiştir. D. scruposus etanol ekstresinin 1 mg’ında 161.4±0.01 µg/mL gallik asit eşdeğeri toplam fenolik bileşikleri bulunmaktadır. Liken ekstresinin antibakteriyal aktivitesi agar kuyu difüzyon yöntemine göre belirlenmiştir. Maksimum antibakteriyal aktivite Bacillus subtilis’e, en düşük antibakteriyal aktivite ise Escherichia coli’ye karşı gözlenmiştir. Bu çalışma, D. scruposus likeninin doğal antioksidan ve antibakteriyal kaynağı olarak kullanılabileceğini göstermiştir

Keywords

• Liken, Serbest radikal süpürme aktivitesi, Toplam fenolik içeriği, Antioksidan aktivite, Antibakteriyal aktivite

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