Interactive effect of static magnetic field and abiotic stressors on growth and biochemical parameters of germinating wheat cultivars

Abstract

In this study, the effects of the individual and combined application of the static magnetic field and

abiotic stressors (60 gL-1 PEG6000 (as a drought stress inducer) or 100 mM NaCI (as a salinity stress

inducer)) on the cultivars of germinating wheat were investigated by assessing growth, biochemical

and antioxidant defence parameters. The seeds of Nina and Flamura-85 species of bread wheat were

exposed to 2.9-4.7 mT static magnetic flow density for a period of 0, 2.2 and 19.8 seconds on a band

turning with the speed of 1 ms-1. Moreover, this magnetic field application was performed together with

abiotic stressors and separately. The growth parameters of both wheat cultivars increased under the static

magnetic field applications, while they decreased when subject to the abiotic stress application than the

control. The application of the static magnetic field together with abiotic stressors statistically increased

the amount of • OH and H2O2 in root samples, and the total Chl, Chl a and Chl b amounts in leaf samples.

The application of the static magnetic field together with abiotic stressors or separately significantly

increased antioxidant enzyme activities (SOD, POD, CAT, APX and GR), and GSH and GSSG content

in all experimental groups when compared to the control. Consequently, that the application of the

magnetic field triggers the antioxidant defence parameters reduced the negative effects of drought and

salinity stresses on the growth parameters in both species.

Keywords

• Antioxidant defence parameters,biochemical parameters,growth parameters,static magnetic field,salinity,drought,wheat

References

1. Aebi H. (1984) Catalase in vitro. Methods Enzymology, 105: 121-6.
2. Bailly C. (2004) Active oxygen species and antioxidants in seed biology. Seed Science Research,14: 93-107.
3. Benzie I.F.F. and Strain J.J. (1996) Ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP. Analytical Biochemistry, 239: 70–6.
4. Bhardwaj J., Anand A. and Nagarajan S. (2012) Biochemical and biophysical changes associated with magnetopriming in germinating cucumber seeds. Plant Physiology and Biochemistry, 57: 67-73.